NIR-SERS studies of DNA and DNA bases attached on polyvinyl alcohol (PVA) protected silver grass-like nanostructures

Analysis of plant genomic DNAs and the genetic relationship among plants by using surface-enhanced Raman spectroscopy

“…The results of our study are in agreement with those of the study by Renming et al [26]. In their study, three strong peaks for DNA adsorbed on grass-like silver nanoparticles and protected with polyvinyl alcohol (excitation wavelength, 785 nm) were observed; they were found at 720, 1010, and 630 cm −1 .…”

“…Other peaks were of weak intensity, and the wavenumber of weak peaks was similar to that found in Ref. [26]. The differences in the findings of this study and their study are that, in this study, the peaks shifted from 720 cm −1 to 717, 715, 714, 713, and 712 cm −1 (mean value: 714.8 cm −1 , shifting by 5.4 cm −1 ); from 1010 cm −1 to 1013, 1011, 1010, and 1009 cm −1 (mean value: 1010.9 cm −1 ; shifting by 0.9 cm −1 ); from 630 cm −1 to 629, 627, 625, 624, and 622 cm −1 (mean value, 625 cm −1 , shifting by 5 cm −1 ).…”

“…Those for guanine are noted at 658 cm −1 (marker peak), 517, 1544, and 1347 cm −1 . The peaks for cytosine appear at 790 cm −1 (marker peak), 1304 cm −1 (marker peak), 1603, 1516, 450, and 649 cm −1 [26]. In this study, the peak at 715 cm −1 was very intense and was the strongest of all peaks.…”

“…The peak at 625 cm −1 was not only assigned to C3 endo/anti deoxyribose but also to the vibration of cytosine and guanine [26]. The peak at 1206 cm −1 was due to the stretching vibration of cytosine and thymine.…”

Análisis de ADN mediante espectroscopía Raman utilizando el método SERS

“…En el área biológica, la espectroscopía Raman se ha utilizado en el análisis de células, tejido y microorganismos [1]. Específicamente, en el análisis de células bacterianas es posible obtener información estructural que permite identificar las células, e inclusive, el diferenciar células normales de células que presenten alguna patología, como el cáncer [2].…”