Analysis of plant genomic DNAs and the genetic relationship among plants by using surface-enhanced Raman spectroscopy

Qiu, Lu; Liu, Peng; Zhao, Li; Wen, Mei-Qiong; Yang, Haiyan; Fan, Shu-Guo; Zhou, Linzong

doi:10.1016/j.vibspec.2014.03.006

Cited by 21 publications

(24 citation statements)

References 39 publications

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“…Besides, we suppose that the high intensity SERS mode near 1364 cm À1 , characterized by a very stable wavenumber for all the analyzed DNA spectra is due to ring stretching modes in dT, dA and dG residues [27,[29][30][31]54,55]. This band was found previously by us to appear around 1356 cm À1 in the SERS spectra of DNAs extracted from Epilobium and Rosa leaves, respectively [4].…”

Section: Resultssupporting

confidence: 57%

“…1 and Table 2) [6]. Also, the intense SERS spectral feature, appearing between 1650 and 1652 cm À1 in all spectra of genomic DNAs from tomato leaves, respectively, is mostly due to dT(C@O) and d(H 2 O) vibrations ( [6,[26][27][28]30] and references therein).…”

Section: Resultsmentioning

confidence: 91%

“…1 indicate wavenumber values for the most prominent bands in each spectrum (in cm À1 units). Table 2 presents the wavenumbers of the surface-enhanced Raman scattering bands, which correspond to each spectrum of the investigated genomic DNA from tomato cultivars [23,[26][27][28][29][30][31]. Proposed SERS band assignments found in the literature for similar compounds are also included.…”

Section: Resultsmentioning

confidence: 99%

“…This spectral contribution is due to localized symmetric stretching vibration of the phosphodioxy (PO 2 À ) moiety and is independent of the base composition and sequence [6,23,[26][27][28][29][30][43][44][45][46][47][48][49][50][51]. The absence of this band from the Darsirius cultivar DNA spectrum can be a consequence of the adsorption of DNA to the metallic surface of silver nanoparticles [6].…”

Section: Resultsmentioning

confidence: 99%

“…cultivars, respectively, in non-cryopreserved controls (C) and after plant cryopreservation (LN) [ The marker band found between 1573 and 1576 cm À1 (Fig. 1) is assigned to ring stretching vibrational modes in dG and dA residues [23,[26][27][28][29][30].…”

Section: Resultsmentioning

confidence: 99%

See 4 more Smart Citations

Surface-enhanced Raman spectroscopy of genomic DNA from in vitro grown tomato (Lycopersicon esculentum Mill.) cultivars before and after plant cryopreservation

Muntean

Leopold

Tripon

et al. 2015

Spectrochimica Acta Part A: Molecular and Biomolecular Spectros

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Section: Resultssupporting

confidence: 57%

Section: Resultsmentioning

confidence: 91%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

See 3 more Smart Citations

Surface-enhanced Raman spectroscopy of genomic DNA from in vitro grown tomato (Lycopersicon esculentum Mill.) cultivars before and after plant cryopreservation

Muntean

Leopold

Tripon

et al. 2015

Spectrochimica Acta Part A: Molecular and Biomolecular Spectros

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Identification of aqueous pollen extracts using surface enhanced Raman scattering (SERS) and pattern recognition methods

Seifert

Merk

Kneipp

2015

Journal of Biophotonics

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Aqueous pollen extracts of varying taxonomic relations were analyzed with surface enhanced Raman scattering (SERS) by using gold nanoparticles in aqueous suspensions as SERS substrate. This enables a selective vibrational characterization of the pollen water soluble fraction (mostly cellular components) devoid of the spectral contributions from the insoluble sporopollenin outer layer. The spectra of the pollen extracts are species-specific, and the chemical fingerprints can be exploited to achieve a classification that can distinguish between different species of the same genus. In the simple experimental procedure, several thousands of spectra per species are generated. Using an artificial neural network (ANN), it is demonstrated that analysis of the intrinsic biochemical information of the pollen cells in the SERS data enables the identification of pollen from different plant species at high accuracy. The ANN extracts the taxonomically-relevant information from the data in spite of high intra-species spectral variation caused by signal fluctuations and preparation specifics. The results show that SERS can be used for the reliable characterization and identification of pollen samples. They have implications for improved investigation of pollen physiology and for allergy warning.

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Raman spectroscopic probing of plant material using SERS

Palanco

Mogensen

Kneipp

2015

J Raman Spectroscopy

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We report surface-enhanced Raman studies on intact plant material using onion layers as a biological target, and silver nanoaggregates and silver island films as enhancing plasmonic structures. Surface-enhanced Raman scattering (SERS) enhancement allows the detection of strong Raman signatures of chemical constituents of the surface of the onion layer such as cellulose, proteins, and flavonols. Because of long-time incubation, SERS sensors can access the extracellular space in the inner of the layer. The location of silver nanoparticles inside the onion layer has been monitored by the SERS images collected from chemicals present in the onion and/or reporter molecules attached to the nanoparticles. Our studies show a competitive adsorption of intrinsic bio molecules of the onion layer and reporter molecules. Different spectra from different places of the layer indicate the complex heterogeneous chemical structure of the plant material. The pH-sensitive reporter molecule para mercapto benzoic acid attached to the nanoparticles allows us to infer pH values inside the extracellular matrix of the onion layer.

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Analysis of plant genomic DNAs and the genetic relationship among plants by using surface-enhanced Raman spectroscopy

Cited by 21 publications

References 39 publications

Surface-enhanced Raman spectroscopy of genomic DNA from in vitro grown tomato (Lycopersicon esculentum Mill.) cultivars before and after plant cryopreservation

Surface-enhanced Raman spectroscopy of genomic DNA from in vitro grown tomato (Lycopersicon esculentum Mill.) cultivars before and after plant cryopreservation

Identification of aqueous pollen extracts using surface enhanced Raman scattering (SERS) and pattern recognition methods

Raman spectroscopic probing of plant material using SERS

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