Nicotine Does Not Affect Vascularization But Inhibits Growth of Freely Transplanted Ovarian Follicles by Inducing Granulosa Cell Apoptosis

Bordel, R.; Laschke, MW; Menger, M.D.; Vollmar, Brigitte

doi:10.1097/01.ogx.0000234792.09557.b9

Cited by 10 publications

(18 citation statements)

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“…During the 7 days after transplantation, nicotine caused a dose-dependent inhibition of follicular growth. They also performed immunohistochemistry for cleaved caspase-3, which revealed a large extent of granulosa cell apoptosis within transplanted follicles of high-dose nicotine-treated animals, while follicles of low-dose nicotine-treated animals presented with only some cells positive for cleaved caspase-3 [10].…”

Section: Pro-apoptotic In Vivo Effectsmentioning

confidence: 99%

Nicotine and apoptosis

Zeidler¹,

Albermann

Lang

2007

Apoptosis

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Cigarette smoking is associated with a plethora of different diseases. Nicotine is the addictive component of cigarette but also acts onto cells of the non-neuronal system, including immune effector cells. Although nicotine itself is usually not referred to as a carcinogen, there is ongoing debate whether nicotine functions as a 'tumor enhancer.' By binding to nicotinic acetylcholine receptors, nicotine deregulates essential biological processes like angiogenesis, apoptosis, and cell-mediated immunity. Apoptosis plays critical roles in a wide variety of physiologic processes during fetal development and in adult tissue and is also a fundamental aspect of the biology of malignant diseases. This review provides an overlook how nicotine influences apoptotic processes and is thus directly involved in the etiology of pathological conditions like cancer and obstructive diseases.

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Section: Pro-apoptotic In Vivo Effectsmentioning

confidence: 99%

Nicotine and apoptosis

Zeidler¹,

Albermann

Lang

2007

Apoptosis

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“…the vascular sprouting, network formation and vessel maturation, as it is described for other free transplanted tissues (56)(57)(58), neonatal, resected and adult transplants showed the same occurrence order, starting with sprouting of vessels from both, the transplant and the host tissue, subsequent network formation and finally maturation, i.e. perfusion of the newly developed vasculature.…”

Section: Discussionmentioning

confidence: 85%

External Inosculation as a Feature of Revascularization Occurs After Free Transplantation of Murine Liver Grafts

Kuehl

Abshagen

Eipel

et al. 2013

American Journal of Transplantation

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The induction of angiogenesis is essential for successful engraftment of freely transplanted cells or cellular composites. How to augment angiogenesis to ensure an appropriate viability of the grafts is still under investigation. This study evaluated the proangiogenic capability of different syngeneic free liver transplants and elucidated the origin of the newly formed vascular network via use of an eGFP + /eGFP − (enhanced green fluorescent protein) cross-over design. Using intravital fluorescence microscopy, we found that neonatal and resected murine liver transplants implanted into dorsal skinfold chambers display a significantly enhanced vascularization compared to regular adult transplants. Immunohistochemically, less tissue hypoxia, apoptosis and macrophage infiltration was observed in the neonatal and resected transplants, which is in line with improved vascularization of those grafts. Additionally, electron microscopy revealed morphological hallmarks of liver cells. eGFP + liver transplants implanted on eGFP − recipients displayed vascular sprouting from the grafts themselves and connection to the recipients`microvasculature, which also undergoes transient proangiogenic response. This process is described as external inosculation, with microvessels exhibiting a chimeric nature of the endothelial lining. These data collectively show that proliferative stimulation is taking effect on angiogenic properties of free transplants and might provide a novel tool for modulating the revascularization of free grafts. Key words: Angiogenesis, GFP+ transgenic mice, intravital microscopy, liver cell transplantation Abbreviations: AP, alkaline-phosphatase; eGFP, enhanced green fluorescent protein; HBSS, Hanks`balanced salt solution; H&E, hematoxylin and eosin; HIF1alpha, hypoxia-inducible factor-1 alpha; HRP, horseradish peroxidase; FITC, fluorescein isothiocyanate; MARS, molecular adsorbents recirculating system; ROI, region of interest; vRBC, red blood cell velocity; VQ, volumetric blood flow.

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“…Second, granulosa are responsible for transferring signals such as FSH, as well as nutrients into and out of the oocyte and between follicle cells during folliculogenesis (Behrman et al, 1988;Fair, 2003;Kidder & Mhawi, 2002;Zhuo & Kimata, 2001). A previous study revealed that cigarette smoke compromised normal functions of granulosa cells and caused cell death in some cases (Bordel et al, 2006;Sinko et al, 2005;Tiemann, Pohland & Schneider, 1996). Therefore, we could not rule out the possibility that oocyte maturation was perturbed due to interference of granulosa functioning.…”

Section: Discussionmentioning

confidence: 97%

In vitro assessment of reproductive toxicity of cigarette smoke and deleterious consequences of maternal exposure to its constituents

Liu

2012

Biol. Res.

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Cigarette smoke is known to be a serious health risk factor and considered reproductively toxic. In the current study, we investigated whether constituents of cigarette smoke, pyrazine, 2-ethylpyridine, and 3-ethylpyridine, adversely aff ect reproductive functioning such as oocyte maturation and sperm capacitation. Our fi ndings indicated that three smoke components were involved in retardation of oocyte maturation in a dose-dependent manner and the lowest-observed-adverse-eff ect level (LOAEL) was determined to be 10 -10 M. However, individual smoke components administrated at the LOAEL did not attenuate oocyte maturation, demonstrating that all three toxicants were equally required for the observed growth impairment. When exposed to all three components at 10 -10 M during in vitro capacitation, murine sperm lost forward progression and were unable to show adequate hyperactivation, which is indicative of the incompletion of the capacitation process. Only sperm administrated with 3-ethylpyridine alone showed signifi cant reduction in capacitation status, suggesting the chemical is the one responsible for disrupting sperm capacitation. Taken together, this is the fi rst report that documents the eff ect of cigarette smoke components on oocyte maturation and sperm capacitation. The present fi ndings demonstrate the adverse eff ects of smoke constituents of mammalian reproduction and the diff erences in sensitivity to smoke components between male and female gametes. Since both processes take place in the female reproductive system, our data provide new insights into deleterious consequences of maternal exposure to cigarette smoke.

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Nicotine Does Not Affect Vascularization But Inhibits Growth of Freely Transplanted Ovarian Follicles by Inducing Granulosa Cell Apoptosis

Cited by 10 publications

References 0 publications

Nicotine and apoptosis

Nicotine and apoptosis

External Inosculation as a Feature of Revascularization Occurs After Free Transplantation of Murine Liver Grafts

In vitro assessment of reproductive toxicity of cigarette smoke and deleterious consequences of maternal exposure to its constituents

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