Nicking enzyme-assisted biosensor for Salmonella enteritidis detection based on fluorescence resonance energy transfer

Song, Yang; Li, Wenkai; Duan, Yingfen; Li, Zhongjie; Deng, Le

doi:10.1016/j.bios.2013.12.053

Cited by 46 publications

(17 citation statements)

References 25 publications

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“…Nanoparticles enhanced Quartz crystal microbalance (QCM)-based immunosensor for Cj was also reported achieving a LOD of 150 cfu/ml [29]. Song et al [30] have achieved a LOD of 100 cfu/ml for Se detection using a FRETbased biosensor which is comparable with our visual LOD. Another fluorescence-based aptasensor on graphene oxide for Se has realised a LOD of 40 cfu/ml [31].…”

Section: Sandwich Colorimetric Lactoferrin Immunoassaysupporting

confidence: 76%

Rapid colorimetric lactoferrin-based sandwich immunoassay on cotton swabs for the detection of foodborne pathogenic bacteria

Alamer

Eissa

Chinnappan

et al. 2018

Talanta

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Cotton swab is the conventional swabbing tool that is usually applied for collecting pathogens from contaminated surfaces, followed by cells lysis and DNA extraction before subjecting to genetic analysis. However, such an approach is time consuming as it involves several steps and requires highly trained personnel to perform the experiment. In this study, we developed a new cotton swab-based detection system that involved integrating bacterial collection, preconcentration and detection on Q-tips. The platform is based on a sandwich assay that can detect different pathogens visually by color changes. Lactoferrin-immobilized cotton is used as a general capturing tool to collect various pathogens from surfaces. The presence of particular bacteria is then detected by immersing the cotton in antibodies attached to different colored nanobeads. The target cell is captured between the lactoferrin and specific antibody-conjugated beads which results in certain color development. The effectiveness of this simply fabricated sensor was demonstrated using Salmonella typhimurium, Salmonella enteritidis, Staphylococcus aureus and Campylobacter jejuni. The intensity of the color on the cotton surfaces increased with increasing the concentration of the pathogenic bacteria. The detection limit was as low as 10 cfu/ml for Salmonella typhimurium and Campylobacter jejuni, 100 cfu/ml for Salmonella enteritidis and 100 cfu/ml for Staphylococcus aureus on chicken meat surface. Moreover, this method showed high selectivity and was further confirmed by loop-mediated isothermal amplification (LAMP). The simplicity and the low cost of this colorimetric sensor renders it applicable to a wide range of other pathogens on different surfaces.

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Section: Sandwich Colorimetric Lactoferrin Immunoassaysupporting

confidence: 76%

Rapid colorimetric lactoferrin-based sandwich immunoassay on cotton swabs for the detection of foodborne pathogenic bacteria

Alamer

Eissa

Chinnappan

et al. 2018

Talanta

View full text Add to dashboard Cite

show abstract

“…In order to improve the detection sensitivity, nucleases are commonly incorporated into the detection process. Song, Li, Duan, Li, and Deng (2014) used DNA nicking endonuclease to recycle nucleic acid hybridization and enzymatic cleavage, leading to the cleavage of numerous molecular beacons. The fluorescence of carbon nanoparticles modified on molecular beacons that was initially quenched by black hole quencher 1 could be restored and greatly amplified.…”

Section: Biosensors With Different Transduces For Detection Of Salmonmentioning

confidence: 99%

Biosensors for rapid detection of Salmonella in food: A review

Shen

2020

Comp Rev Food Sci Food Safe

141

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Salmonella is one of the main causes of foodborne infectious diseases, posing a serious threat to public health. It can enter the food supply chain at various stages of production, processing, distribution, and marketing. High prevalence of Salmonella necessitates efficient and effective approaches for its identification, detection, and monitoring at an early stage. Because conventional methods based on plate counting and real‐time polymerase chain reaction are time‐consuming and laborious, novel rapid detection methods are urgently needed for in‐field and on‐line applications. Biosensors provide many advantages over conventional laboratory assays in terms of sensitivity, specificity, and accuracy, and show superiority in rapid response and potential portability. They are now recognized as promising alternative tools and one of the most on‐site applicable and end user–accessible methods for rapid detection. In recent years, we have witnessed a flourishing of studies in the development of robust and elaborate biosensors for detection of Salmonella in food. This review aims to provide a comprehensive overview on Salmonella biosensors by highlighting different signal‐transducing mechanisms (optical, electrochemical, piezoelectric, etc.) and critically analyzing its recent trends, particularly in combination with nanomaterials, microfluidics, portable instruments, and smartphones. Furthermore, current challenges are emphasized and future perspectives are discussed.

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“…Song et al also developed a biosensor for the detection of S . Enteritidis by FRET using a nicking enzyme and carbon nanoparticles (CNPs) [142]. The surface of the CNPs was modified by the grafting of a particular ssDNA containing two consecutive sequences.…”

Section: Biosensors For Salmonella Detection and Quantificationmentioning

confidence: 99%

Detection of Salmonella in Food Matrices, from Conventional Methods to Recent Aptamer-Sensing Technologies

Paniel

Noguer

2019

Foods

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Rapid detection of the foodborne pathogen Salmonella in food processing is of crucial importance to prevent food outbreaks and to ensure consumer safety. Detection and quantification of Salmonella species in food samples is routinely performed using conventional culture-based techniques, which are labor intensive, involve well-trained personnel, and are unsuitable for on-site and high-throughput analysis. To overcome these drawbacks, many research teams have developed alternative methods like biosensors, and more particularly aptasensors, were a nucleic acid is used as biorecognition element. The increasing interest in these devices is related to their high specificity, convenience, and relative rapid response. This review aims to present the advances made in these last years in the development of biosensors for the detection and the quantification of Salmonella, highlighting applications on meat from the chicken food chain.

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Nicking enzyme-assisted biosensor for Salmonella enteritidis detection based on fluorescence resonance energy transfer

Cited by 46 publications

References 25 publications

Rapid colorimetric lactoferrin-based sandwich immunoassay on cotton swabs for the detection of foodborne pathogenic bacteria

Rapid colorimetric lactoferrin-based sandwich immunoassay on cotton swabs for the detection of foodborne pathogenic bacteria

Biosensors for rapid detection of Salmonella in food: A review

Detection of Salmonella in Food Matrices, from Conventional Methods to Recent Aptamer-Sensing Technologies

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