Detection of Salmonella in Food Matrices, from Conventional Methods to Recent Aptamer-Sensing Technologies

Paniel, Nathalie; Noguer, Thierry

doi:10.3390/foods8090371

Cited by 75 publications

(40 citation statements)

References 194 publications

(283 reference statements)

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“…Culture-based methods are a subtle but time-intensive process [ 43 ]. Some bacterial species require an enrichment broth or buffer before their isolation on differential media and serological confirmation [ 44 ]. Numerous chicken-borne pathogenic bacteria species confirmed via a culture-based method have been enlisted in Table 3 .…”

Section: Conventional Methods Of Microbial Detectionmentioning

confidence: 99%

Detection of Bacterial Pathogens and Antibiotic Residues in Chicken Meat: A Review

Kumar

Bhardwaj

Kaur

et al. 2020

Foods

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Detection of pathogenic microbes as well as antibiotic residues in food animals, especially in chicken, has become a matter of food security worldwide. The association of various pathogenic bacteria in different diseases and selective pressure induced by accumulated antibiotic residue to develop antibiotic resistance is also emerging as the threat to human health. These challenges have made the containment of pathogenic bacteria and early detection of antibiotic residue highly crucial for robust and precise detection. However, the traditional culture-based approaches are well-comprehended for identifying microbes. Nevertheless, because they are inadequate, time-consuming and laborious, these conventional methods are not predominantly used. Therefore, it has become essential to explore alternatives for the easy and robust detection of pathogenic microbes and antibiotic residue in the food source. Presently, different monitoring, as well as detection techniques like PCR-based, assay (nucleic acid)-based, enzyme-linked immunosorbent assays (ELISA)-based, aptamer-based, biosensor-based, matrix-assisted laser desorption/ionization-time of flight mass spectrometry-based and electronic nose-based methods, have been developed for detecting the presence of bacterial contaminants and antibiotic residues. The current review intends to summarize the different techniques and underline the potential of every method used for the detection of bacterial pathogens and antibiotic residue in chicken meat.

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Section: Conventional Methods Of Microbial Detectionmentioning

confidence: 99%

Detection of Bacterial Pathogens and Antibiotic Residues in Chicken Meat: A Review

Kumar

Bhardwaj

Kaur

et al. 2020

Foods

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“…The fundamental parts of a biosensor are shown in Figure 4 . Biological recognition elements used in microbial sensing include antibodies, toxins, nucleic acids, whole cells, and biomimetic materials [ 70 , 71 , 72 ].…”

Section: Emerging Biosensorsmentioning

confidence: 99%

Recent Advances in the Detection of Antibiotic and Multi-Drug Resistant Salmonella: An Update

Hulme

2021

IJMS

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Antibiotic and multi-drug resistant (MDR) Salmonella poses a significant threat to public health due to its ability to colonize animals (cold and warm-blooded) and contaminate freshwater supplies. Monitoring antibiotic resistant Salmonella is traditionally costly, involving the application of phenotypic and genotypic tests over several days. However, with the introduction of cheaper semi-automated devices in the last decade, strain detection and identification times have significantly fallen. This, in turn, has led to efficiently regulated food production systems and further reductions in food safety hazards. This review highlights current and emerging technologies used in the detection of antibiotic resistant and MDR Salmonella.

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“…Salmonella is the second most common bacterium in human gastrointestinal outbreaks in the European Union, especially Salmonella Enteritidis and Salmonella typhimurium . In China, from 1994 to 2005, Salmonella was the main cause of bacterial foodborne diseases outbreaks (Gong et al., 2017; Paniel & Noguer, 2019).…”

Section: Introductionmentioning

confidence: 99%

Sensitive detection of foodborne pathogens based on CRISPR‐Cas13a

Gao

et al. 2021

Journal of Food Science

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Salmonella, being one of the most widespread foodborne pathogens, is a compulsory test item required by national food safety standard of China and many other countries. More sensitive and specific Salmonella detection method is still needed since traditional methods are time consuming and highly dependent on enormous manpower and material resources. In this research, a bacteria detection method based on CRISPR‐Cas13a system (where CRISPR is Clustered Regularly Interspaced Short Palindromic Repeats) was proposed. The target DNA was amplified by PCR and transcribed into RNA by T7 transcriptase, which can activate the RNase activity of the Cas13a protein. The self‐folding quenched fluorescent probe can be cleaved by the activated Cas13a protein to generate fluorescent signal. We named this method as PCF detection (PCR‐CRISPR‐Fluorescence based nucleic acid detection). In this study, PCF detection showed excellent sensitivity, which can detect Salmonella genomic DNA with a minimum of 101 aM or 10° CFU/ml Salmonella bacteria in 2 hr. It also showed good specificity with no cross‐reaction with other common foodborne bacteria. Practical Application The PCF detection method proposed in this article can detect Salmonella sensitively and specifically, providing a novel strategy for the detection of foodborne pathogens in food and has great application potential in other microbial detection fields.

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Detection of Salmonella in Food Matrices, from Conventional Methods to Recent Aptamer-Sensing Technologies

Cited by 75 publications

References 194 publications

Detection of Bacterial Pathogens and Antibiotic Residues in Chicken Meat: A Review

Detection of Bacterial Pathogens and Antibiotic Residues in Chicken Meat: A Review

Recent Advances in the Detection of Antibiotic and Multi-Drug Resistant Salmonella: An Update

Sensitive detection of foodborne pathogens based on CRISPR‐Cas13a

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