Nickel, palladium and rhodium induced IFN-gamma and IL-10 production as assessed by in vitro ELISpot-analysis in contact dermatitis patients

Bordignon, Valentina; Palamara, Francesca; Cordiali‐Fei, Paola; Vento, Antonella; Aiello, Arianna; Picardo, Mauro; Ensoli, Fabrizio; Cristaudo, Antonio

doi:10.1186/1471-2172-9-19

Cited by 45 publications

(38 citation statements)

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“…However, the lymphocyte transformation test can only be used in the diagnosis of nickel allergies on account of the low specificity and sensitivity of the test for other metals [9]. Enzymatic assays have also shown promise as diagnostic tools for ACD [10,11,12]. In particular, an ELISpot assay, which can detect serum levels of IL-4, IL-5, IL-10, IL-13, and IFN-γ, was developed for ACD diagnostics [13].…”

Section: Introductionmentioning

confidence: 99%

Serum Biomarkers of Allergic Contact Dermatitis: A Pilot Study

Zinkevičienė

Kainov²,

Lastauskienė³

et al. 2015

Int Arch Allergy Immunol

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Background: Allergic contact dermatitis (ACD) is an inflammatory skin disease caused by repeated skin exposure to contact allergens. The goal of this pilot study was to identify inflammatory proteins which can serve as biomarkers for ACD. Methods: We measured levels of 102 cytokines, chemokines, and growth factors in the sera of 16 ACD patients during acute and remission phases, and 16 healthy volunteers. Results: Serumlevels of adiponectin, chemokine (C-C motif) ligand 5 (CCL5), C-reactive protein (CRP), chitinase 3-like 1 (CHI3L1), complement factor D (CFD), endoglin, lipocalin-2, osteopontin, retinol-binding protein 4 (RBP4), and platelet factor 4 (PF4) were significantly higher, whereas levels of trefoil factor 3 (TFF3) were significantly lower, in ACD patients than in healthy controls. In ACD patients, serum levels of CCL5 were elevated, whereas levels of TFF3, soluble intercellular adhesion molecule-1 (sICAM-1), and platelet-derived growth factor (PDGF)-AB/BB were found to be lower during the remission phase of the disease. Conclusions: Serum levels of adiponectin, CCL5, CRP, CHI3L1, CFD, endoglin, lipocalin-2, osteopontin, RBP4, PF4, and TFF3 might be exploited as biomarkers for ACD, whereas levels of CCL5, TFF3, sICAM-1, and PDGF-AB/BB might be exploited for evaluation of disease progression and efficacy of ACD treatment.

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Section: Introductionmentioning

confidence: 99%

Serum Biomarkers of Allergic Contact Dermatitis: A Pilot Study

Zinkevičienė

Kainov²,

Lastauskienė³

et al. 2015

Int Arch Allergy Immunol

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“…In this way the ELISpot can provide information on the numbers of antigen-specific cytokine secreting cells and the amount of secretion can be calculated by the area of the spots for each cell. ELISpot assays are a sensitive and specific in vitro assay for detecting cytokine profiles in response to contact allergens (Bordignon et al, 2008). In patients with ACD to nickel, the ELISpot assay was shown to improve detection of specific T-lymphocyte responses (Spiewak et al, 2007).…”

Section: Allergen-driven Activation and Cytokine Secretionmentioning

confidence: 99%

The lymphocyte transformation test in allergic contact dermatitis: New opportunities

Popple

Williams

Maxwell

et al. 2015

Journal of Immunotoxicology

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Allergic contact dermatitis (ACD) is driven by the activation and proliferation of allergen-specific memory T-lymphocytes and is currently diagnosed by patch testing with a selected panel of chemical allergens. The lymphocyte transformation test (LTT) can be used to monitor ex vivo T-lymphocyte responses to antigens, including contact allergens. The LTT is not viewed as being an alternative to patch testing, but it does seek to reflect experimentally skin sensitization to specific chemicals. The LTT is based on stimulation in vitro of antigendriven T-lymphocyte proliferation. That is, exposure in culture of primed memory T-lymphocytes to the relevant antigen delivered in an appropriate configuration will provoke a secondary response that reflects the acquisition of skin sensitization. The technical aspects of this test and the utility of the approach for investigation of immune responses to contact allergens in humans are reviewed here, with particular emphasis on further development and refinement of the protocol. An important potential application is that it may provide a basis for characterizing those aspects of T-lymphocyte responses to contact allergens that have the greatest influence on skin sensitizing potency and this will be considered in some detail.

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“…In vitro tests such as the macrophage migration test, the lymphocyte blastic transformation test and the lymphocyte transformation test have mostly been used in research projects (Rasanen and Tuomi 1992;Bordignon et al 2008). None of these have become routine analyses in the clinical setting, even when 2 or 3 methods have been combined.…”

Section: Other Tests Availablementioning

confidence: 99%