Niabella hibiscisoli sp. nov., isolated from soil of a Rose of Sharon garden

Ngo, Hien T. T.; Trinh, Huan; Yan, Zheng‐Fei; Moya, Gabriela; Kook, MooChang; Yi, Tae–Hoo

doi:10.1099/ijsem.0.001595

Cited by 10 publications

(6 citation statements)

References 27 publications

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“…These are Gram-negative bacteria, aerobic, non-flagellated, and rod-shaped and they produce flexirubin-type pigments ( Dai et al, 2011 ). There are seven species described ( Glaeser et al, 2013 ) isolated from soils ( Dai et al, 2011 ; Ngo et al, 2017 ), water ( Siddiqi and Im, 2016 ) medicinal leeches ( Kikuchi et al, 2009 ), as well as epiphytic communities in the green macroalgae Cladophoraglomerata ( Zulkifly et al, 2012 ). This bacterium was indeed found associated with leeches and macroalgae, both highly humid environments, just like the toad skin.…”

Section: Discussionmentioning

confidence: 99%

Characterization of the Skin Microbiota of the Cane Toad Rhinella cf. marina in Puerto Rico and Costa Rica

et al. 2018

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Rhinella marina is a toad native to South America that has been introduced in the Antilles, likely carrying high loads of microorganisms, potentially impacting local community diversity. The amphibian skin is involved in pathogen defense and its microbiota has been relatively well studied, however, research focusing on the cane toad microbiota is lacking. We hypothesize that the skin microbial communities will differ between toads inhabiting different geographical regions in Central America and the Caribbean. To test our hypothesis, we compared the microbiota of three populations of R. cf. marina toads, two from Costa Rican (native) and one Puerto Rican (exotic) locations. In Costa Rica, we collected 11 toads, 7 in Sarapiquí and 4 from Turrialba while in Puerto Rico, 10 animals were collected in Santa Ana. Separate swab samples were collected from the dorsal and ventral sites resulting in 42 samples. We found significant differences in the structure of the microbial communities between Puerto Rico and Costa Rica. We detected as much as 35 different phyla; however, communities were dominated by Proteobacteria, Bacteroidetes, Firmicutes, and Actinobacteria. Alpha diversity and richness were significantly higher in toads from Puerto Rico and betadiversity revealed significant differences between the microbiota samples from the two countries. At the genus level, we found in Santa Ana, Puerto Rico, a high dominance of Kokuria , Niabella , and Rhodobacteraceae, while in Costa Rica we found Halomonas and Pseudomonas in Sarapiquí, and Acinetobacter and Citrobacter in Turrialba. This is the first report of Niabella associated with the amphibian skin. The core microbiome represented 128 Operational Taxonomic Units (OTUs) mainly from five genera shared among all samples, which may represent the symbiotic Rhinella ’s skin. These results provide insights into the habitat-induced microbial changes facing this amphibian species. The differences in the microbial diversity in Puerto Rican toads compared to those in Costa Rica provide additional evidence of the geographically induced patterns in the amphibian skin microbiome, and highlight the importance of discussing the microbial tradeoffs in the colonization of new ecosystems.

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Section: Discussionmentioning

confidence: 99%

Characterization of the Skin Microbiota of the Cane Toad Rhinella cf. marina in Puerto Rico and Costa Rica

et al. 2018

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“…Strain EG3 is the first MGPB for E. gracilis . Members of the genus Emticicia have been isolated from various environments, including freshwater [34–37], wetlands [38], seawater [39], the sediments of a shallow stream [40], and soil [41, 42]. Although the ecological role of Emticicia and its potential for industrial use are still unclear, our results reveal that one of its ecosystem services may be to function in a symbiotic relationship that enhances the growth of microalgae such as E. gracilis .…”

Section: Discussionmentioning

confidence: 99%

Enhanced production of biomass and lipids by Euglena gracilis via co-culturing with a microalga growth-promoting bacterium, Emticicia sp. EG3

Toyama

Hanaoka

Yamada³

et al. 2019

Biotechnol Biofuels

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BackgroundEuglena gracilis, a unicellular flagellated microalga, is regarded as one of the most promising species as microalgal feedstock for biofuels. Its lipids (mainly wax esters) are suitable for biodiesel and jet fuel. Culture of E. gracilis using wastewater effluent will improve the economics of E. gracilis biofuel production. Enhancement of the productivity of E. gracilis biomass is critical to creating a highly efficient biofuels production system. Certain bacteria have been found to promote microalgal growth by creating a favorable microenvironment. These bacteria have been characterized as microalgae growth-promoting bacteria (MGPB). Co-culture of microalgae with MGPB might offer an effective strategy to enhance microalgal biomass production in wastewater effluent culture systems. However, no MGPB has been identified to enhance the growth of E. gracilis. The objectives of this study were, therefore, to isolate and characterize the MGPB effective for E. gracilis and to demonstrate that the isolated MGPB indeed enhances the production of biomass and lipids by E. gracilis in wastewater effluent culture system.ResultsA bacterium, Emticicia sp. EG3, which is capable of promoting the growth of microalga E. gracilis, was isolated from an E. gracilis-municipal wastewater effluent culture. Biomass production rate of E. gracilis was enhanced 3.5-fold and 3.1-fold by EG3 in the co-culture system using a medium of heat-sterilized and non-sterilized wastewater effluent, respectively, compared to growth in the same effluent culture but without EG3. Two-step culture system was examined as follows: E. gracilis was cultured with or without EG3 in wastewater effluent in the first step and was further grown in wastewater effluent in the second step. Production yields of biomass and lipids by E. gracilis were enhanced 3.2-fold and 2.9-fold, respectively, in the second step of the system in which E. gracilis was co-cultured with EG3 in the first step.ConclusionEmticicia sp. EG3 is the first MGPB for E. gracilis. Growth-promoting bacteria such as EG3 will be promising agents for enhancing E. gracilis biomass/biofuel productivities.

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“…Catalase activity was determined by bubble production in 3 % (v/v) H 2 O 2 and oxidase activity was determined using 1 % (w/v) N , N , N ′, N ′-tetramethyl-1, 4-phenylenediamine reagent. Tests for degradation of starch, casein, DNA, Tween 20, Tween 80, l -tyrosine, CMC and chitin from crab shells were evaluated after 5 days of incubation at 28 °C as described by [24]. Carbon-source utilization and enzyme activities were tested by using API 20NE, API ID 32 GN and API ZYM test kits according to the instructions of the manufacturer (bioMérieux).…”

Section: Full-textmentioning

confidence: 99%

Aquitalea aquatilis sp. nov., isolated from Jungwon waterfall

Ngo

Kim

Trinh

et al. 2020

International Journal of Systematic and Evolutionary Microbiology

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A Gram-stain-negative, facultative anaerobic, motile, short rods and yellow-pigmented bacterium, designated strain THG-DN7.12T, was isolated from water collected at Jungwon waterfall on Yongmun mountain, Republic of Korea. According to 16S rRNA gene sequence comparisons, strain THG-DN7.12T was found to be most closely related to Aquitalea denitrificans 5YN1-3T (98.9 % sequence similarity), Aquitalea magnusonii TRO-001DR8T (98.7 %) and Aquitalea pelogenes P1297T (98.0 %). The DNA–DNA relatedness between strain THG-DN7.12T and its phylogenetically closest neighbours was below 70.0 %. The strain's DNA G+C content was 59.7 mol%. The major polar lipid was found to be phosphatidylethanolamine. Summed feature 3 (C16 : 1 ω7c and/or C16 : 1 ω6c) and C16 : 0 were identified as the major fatty acids. Ubiquinone Q-8 was detected as the only respiratory quinone. These data supported the affiliation of strain THG-DN7.12T to the genus Aquitalea . Strain THG-DN7.12T was distinguished from related Aquitalea species by physiological and biochemical tests. Therefore, the novel isolate represents a novel species, for which the name Aquitalea aquatilis sp. nov. is proposed, with THG-DN7.12T as the type strain (=KACC 18847T=CCTCC AB 2016185T).

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Niabella hibiscisoli sp. nov., isolated from soil of a Rose of Sharon garden

Cited by 10 publications

References 27 publications

Characterization of the Skin Microbiota of the Cane Toad Rhinella cf. marina in Puerto Rico and Costa Rica

Characterization of the Skin Microbiota of the Cane Toad Rhinella cf. marina in Puerto Rico and Costa Rica

Enhanced production of biomass and lipids by Euglena gracilis via co-culturing with a microalga growth-promoting bacterium, Emticicia sp. EG3

Aquitalea aquatilis sp. nov., isolated from Jungwon waterfall

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