New xylanolytic enzyme from Geobacillus galactosidasius BS61 from a geothermal resource in Turkey

Sari, Bilge; Faiz, Özlem; Genç, Berna; Şişecioğlu, Melda; Adıgüzel, Ahmet; Adıgüzel, Gülşah

doi:10.1016/j.ijbiomac.2018.07.166

Cited by 14 publications

(15 citation statements)

References 56 publications

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“…For DNA extraction of each isolate, a single colony from NA medium was selected and inoculated to NB at 55°C 150 rpm. After the incubation period, genomic DNA isolation was carried out according to Promega WizardR DNA purification kit and purified DNA was stored at +4°C until use 47 . Test isolates were subjected to rep-PCR with the special primers of (GTG) 5 and BOX elements to obtain genomic fingerprinting 4,6,20 .…”

Section: Gene Amplification Cloning and Genomic Fingerprinting Of Ismentioning

confidence: 99%

“…The NaCl requirement for growth was also tested in NB medium containing 1.0, 2.0, 4.0, 6.0, 8.0, 10.0 and 12.0% (w/v) NaCl. Cell and colony morphology (Leica ICC50 HD light microscope), Gram and endospore staining, motility and the presence of catalase and oxidase reactions were also investigated by the method of Prescott 44 and Sari 47 .…”

Section: Conventional Identificationmentioning

confidence: 99%

“…Incubation was carried out at 55°C for two days, and after the incubation, the plates were stained by Congo-Red Dye. The presence of clear zone was accepted as positive 47 . lipase Test isolates were inoculated on tributyrin agar medium which contained 1% (w/v) tributyrin (glycerol tributyrate) and incubated at 55°C.…”

Section: Cellulasementioning

confidence: 99%

“…The plates were incubated at 55°C for four days and after the incubation, the plates were stained by Congo-Red Dye. The presence of clear zone was accepted as positive 47 .…”

Section: Xylanasementioning

confidence: 99%

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Presence of Different Bacterial Species in Thermal Sources and Novelty in Their Industrial Enzyme Productions

Albayrak¹,

Genç²,

Özkan³

et al. 2019

J Pure Appl Microbiol

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In this study, one hundred and thirty isolates were isolated from water and sludge samples taken from hot springs located in different regions of Turkey. Among them, eleven isolates were chosen according to conventional (morphological, physiological and biochemical tests) and molecular methods (rep-PCR and 16S rRNA sequencing). These bacteria were then tested for their capability to produce valuable enzymes. As a result; species belonging to Bacillus, Anoxybacillus, Aeribacillus, Enterococcus, Exiguobacterium and Paenibacillus were identified. Test strains were found to have optimum reproductive potential at pH 5.0-9.0 and 15-65°C, usually at a concentration of 1.0-10.0% (w/v) NaCl. In addition, all thermotolerant bacteria were Gram, endospore (except E. profundum), catalase and oxidase (except E. faecium and E. profundum) positive, and rod-shaped (except E. faecium). It was observed that all isolates had a 99% similarity percentage as a result of 16S rRNA sequence analysis. All of the isolates were capable of producing industrially important enzymes moreover, eight of them could produce at least two of these enzymes. Test strains had high potential of industrial enzyme production, and the enzymes from these thermo-tolerant isolates will be widely used in biotechnological processes.

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Section: Gene Amplification Cloning and Genomic Fingerprinting Of Ismentioning

confidence: 99%

Section: Conventional Identificationmentioning

confidence: 99%

Section: Cellulasementioning

confidence: 99%

“…The plates were incubated at 55°C for four days and after the incubation, the plates were stained by Congo-Red Dye. The presence of clear zone was accepted as positive 47 .…”

Section: Xylanasementioning

confidence: 99%

See 2 more Smart Citations

Presence of Different Bacterial Species in Thermal Sources and Novelty in Their Industrial Enzyme Productions

Albayrak¹,

Genç²,

Özkan³

et al. 2019

J Pure Appl Microbiol

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“…The reasons of enzymes' use are that they have a character of substrate specificity and ordinary reaction conditions, as well as their nontoxic property (Cakmak & Saglam Ertunga, 2016;Shahrestani et al, 2016). Many studies have reported that xylanase is a powerful enzyme for fruit juice extraction and clarification process (Adigüzel & Tunçer, 2016;Bajaj & Manhas, 2012;Cakmak & Saglam Ertunga, 2016;Nagar et al, 2012;Olfa et al, 2007;Sari et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

A novel thermostable xylanase from Geobacillus vulcani GS90: Production, biochemical characterization, and its comparative application in fruit juice enrichment

2021

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Xylanases have great attention to act as a potential role in agro-industrial processes.In this study, production, characterization, and fruit juice application of novel xylanase from thermophilic Geobacillus vulcani GS90 (GvXyl) were performed. GvXyl was purified via acetone precipitation and gel-filtration chromatography. The results showed that GvXyl had 1,671.4 U/mg of specific activity and optimally worked at pH 8 and 55°C. It was also active in a wide pH (3-9) and temperature (30-90ºC) ranges.GvXyl was highly stable at 90ºC and relatively stable at pH 3-9. The kinetic parameters of GvXyl were obtained as K m , V max , and k cat ; 10.2 mg/ml, 4,104 µmol min −1 mg −1 , and 3,542.6 s −1 , respectively. GvXyl had higher action than commercial xylanase in fruit juice enrichment. These results revealed that GvXyl might possess a potential influence in fruit juice processing because of its high specific activity and great thermal stability.

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Purification and Biochemical Characterization of a Novel Thermostable Serine Protease from Geobacillus sp. GS53

Baykara

Sürmeli

Şanlı‐Mohamed

2021

Appl Biochem Biotechnol

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New xylanolytic enzyme from Geobacillus galactosidasius BS61 from a geothermal resource in Turkey

Cited by 14 publications

References 56 publications

Presence of Different Bacterial Species in Thermal Sources and Novelty in Their Industrial Enzyme Productions

Presence of Different Bacterial Species in Thermal Sources and Novelty in Their Industrial Enzyme Productions

A novel thermostable xylanase from Geobacillus vulcani GS90: Production, biochemical characterization, and its comparative application in fruit juice enrichment

Purification and Biochemical Characterization of a Novel Thermostable Serine Protease from Geobacillus sp. GS53

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