New Techniques for Rapid Cryopreservation of Dormant Vegetative Buds

Kovalchuk, Irina; Turdiev, Timur; Mukhitdinova, Zinat; Frolov, S.; Reed, Barbara M.; Kairova, Gulshariya

doi:10.17660/actahortic.2014.1039.17

Cited by 9 publications

(3 citation statements)

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“…Differences in cooling rates, at 5°C/day compared to 1°C/h, and transfer to -35°C before cryopreservation showed higher viability for some accessions (Towill and Bonnart 2005). Kovalchuk et al (2014) investigated the influence of different combinations of pretreatments and cryoprotectants on the success of recovery of dormant apple buds. The application of IPBB (50 % glycerol and 50 % glucose) gave 100 % viability for four tested apple genotypes by staining with 2, 3, 5-Triphenyltetrazolium chloride.…”

Section: Discussionmentioning

confidence: 99%

Cryopreservation of winter-dormant apple buds: establishment of a duplicate collection of Malus germplasm

Höfer

2015

Plant Cell Tiss Organ Cult

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The clonal wild-species Malus collection at the Julius Kühn-Institute, Institute for Breeding Research on Fruit Crops, Germany, comprises 507 accessions of 46 species and represents one of the largest collections in Europe. Cryopreservation was applied to 77 accessions of 31 species and hybrids in the Malus collection using a winter vegetative-bud method. No significant differences were observed in the recovery of the two controls, untreated buds and desiccated buds, however, cryopreservation significantly decreased bud recovery, independent of the rootstock used for chip budding. Data from this investigation showed an effect on recovery of year and genotype between and within species. Of the 77 accessions, 62 had living buds, and recovery was [40 % for 37 and these averaged 68.5 % recovery. The initial moisture content, dehydration time, and morphology of the buds had no significant influence on recovery. The higher percentage of recovery in one experimental year was possibly the result of secondary buds that grew despite lethal injury to the primary meristems. Adaptations to the general protocol and the handling details were successful, and the cryopreservation of dormant buds will now be part of the conservation strategy for Malus genetic resources in Germany.Cryopreservation will be a part of the conservation strategy of Malus genetic resources in Germany. Effect of moisture content, dehydration time, morphology of buds, rootstock and genotype are discussed.

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Section: Discussionmentioning

confidence: 99%

Cryopreservation of winter-dormant apple buds: establishment of a duplicate collection of Malus germplasm

Höfer

2015

Plant Cell Tiss Organ Cult

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“…for Morus (Yakuwa and Oka 1988,) Pyrus (Oka et al 1991), Betula (Ryynänen 1996), Diospyros (Matsumoto et al 2004), Populus (Jokipii et al 2004,) and Ulmus (Harvengt et al2004). The effects of different pre-treatment combinations and cryoprotectants for dormant bud cryopreservation have also been investigated (Kovalchuk et al 2014;Zhumagulova et al 2014).…”

Section: Introductionmentioning

confidence: 99%

Cryopreservation of Ribes nigrum (L.) dormant buds: recovery via in vitro culture to the field

Rantala

Kaseva

Karhu

et al. 2019

Plant Cell Tiss Organ Cult

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Cryopreservation of dormant buds can be a feasible method for long term preservation of clonally propagated woody plants.In the present study, dormant buds of blackcurrant (Ribes nigrum L) cultivar Mortti were cryopreserved. Twig segments from greenhouse and field grown plants were cooled at their natural moisture content at the rate of 0.17 °C min −1 from zero to −38 °C, immersed in liquid nitrogen and stored in liquid nitrogen vapor. The post-cryopreservation regrowth of buds was evaluated in vitro and viability of recovered plants was tested in vivo. The estimated recovery rate for buds thawed after 1-6 days in cryostorage was 86% and 66% for outdoor and greenhouse grown buds, respectively. Microplants adequate in size and quality grew successfully in vivo and plants produced berries in field. After 4 years in cryostorage, the estimated recovery rate for outdoor buds was 58%. According to results from 1 to 6 days cryostorage, the tested dormant bud protocol turned to be applicable for cryopreservation of the blackcurrant cultivar Mortti. However, concerning the long-term preservation the decline of recovery is important to consider in the future experiments. Key messageThe cryopreservation of dormant buds combined with in vitro recovery provides a feasible tool for conservation of plant genetic resources of blackcurrant.

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“…In Europe, cryopreservation of apple dormant buds was done from the North, e.g., Finland – Salix ( Ryynanen, 1996 ) to the South, e.g., Italy ( Lambardi, 2012 ) from the West, e.g., Denmark ( Toldam-Andersen et al, 2007 ; Vogiatzi et al, 2011 ) to the East, e.g., Germany ( Hofer, 2007 , 2015 ), and The Czech Republic ( Zamecnik et al, 2007 ). Dormant bud cryopreservation started to develop also in Kazachstan ( Kovalchuk et al, 2014 ) and other Asian regions. In all these cryopreservation centers on different continents with different winter climates, the donor trees of buds for cryopreservation are supposed to be in a dormant state (they do not distinguish different parts of dormancy) even though their cryopreservation results vary with season.…”

Section: Introductionmentioning

confidence: 99%

Cryotolerance of apple tree bud is independent of endodormancy

2015

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Increasing interest in cryopreservation of dormant buds reveals the need for better understanding of the role of dormancy in cryotolerance. Dormancy stage and low-temperature survival of vegetative apple buds (Malus domestica Borkh.), cultivars ‘Sampion’ and ‘Spartan’, collected from orchard were evaluated during three seasons contrasting in temperature and precipitation throughout the arrested plant growth period. During each season, the cultivars differed either in the onset of the endodormancy or in the length of the endodormant period. A simple relation between endodormancy of the buds and their water content was not detected. The cryosurvival of vegetative apple buds of both cultivars correlated with their cold hardening without direct regard to their particular phase of dormancy. The period of the highest bud cryotolerance after low-temperature exposure overlapped with the endodormant period in some evaluated seasons. Both cultivars had the highest cryosurvival in December and January. The presented data were compared with our previous results from a dormancy study of in vitro apple culture. Endodormancy coincided with the period of successful cryosurvival of apple buds after liquid nitrogen exposure, but as such, it was not decisive for their survival and did not limit their successful cryopreservation.

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New Techniques for Rapid Cryopreservation of Dormant Vegetative Buds

Cited by 9 publications

References 0 publications

Cryopreservation of winter-dormant apple buds: establishment of a duplicate collection of Malus germplasm

Cryopreservation of winter-dormant apple buds: establishment of a duplicate collection of Malus germplasm

Cryopreservation of Ribes nigrum (L.) dormant buds: recovery via in vitro culture to the field

Cryotolerance of apple tree bud is independent of endodormancy

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