New spectrophotometric microdetermination of carbapenem antibiotics derivatives in pharmaceutical formulations

Abstract

A new sensitive spectrophotometric method was developed to determine three carbapenem antibiotics: imipenem, meropenem and ertapenem. The proposed method was based on the formation of the coloured tris(o-phenanthroline)-iron(II) complex (ferroin) [Phen] or Fe (II)-2,2 -bipyridyl complex [Bip] in the reaction of the tested drugs with the corresponding iron (III)-complexes in an acetate pH 4 buffer. The formed coloured complexes showed maximum absorbance at 510 and 520 nm for [Phen] and [Bip], respectively. The reaction conditions, including the pH, reagent concentration, reaction time, temperature and stability of the formed coloured species, were optimized to achieve the highest sensitivity. Linear calibration curves were obtained in the concentration ranges from 0.2 to 10, 0.5 to 10 and 0.5 to 10 g mL −1 for the aforementioned drugs in the same order. The developed method was successfully applied to determine the investigated carbapenems in their pharmaceutical formulations with average recoveries of 100.8, 99.8 and 99.4% for the Phen method and 98.9, 101.7 and 100.6% for the Bip method for imipenem, meropenem and ertapenem, respectively. A statistical comparison of the results with the reference method showed good concurrence and indicated no significant difference in accuracy or precision.

Challenge Approach of an Inexpensive Electrochemical Sensor for Rapid Selective Determination of two Non‐classical β‐Lactams in Presence of Different Degradants and Interference Substances

“…The surface areas of the compared GPE and GCE were obtained by applying cyclic voltammetry using 1.0 mM K 4 Fe (CN) 6 as a probe at different scan rates. For a reversible process, the following Randles-Sevcik formula was used [28]:…”

“…Where I pa refers to the anodic peak current, n is the number of electrons transferred, A 0 is the surface area of the electrode, D 0 is the diffusion coefficient, u is the scan rate (V.s À1 ) and C 0 is the concentration of K 4 Fe (CN) 6 . For 1.0 mM K 4 Fe (CN) 6 , n= 1, D o = 7.6 3 10 À6 cm 2 s À1 , then from the slope of the plot of I pa vs. u 1/2 , the electro-active area was calculated [29,30]. In our experiment, electroactive areas of GPE and GCE were found to be 0.2207 and 0.118 cm 2 .…”

“…The literature survey comprised several analytical methods for quantitative determination of (MP) and (EP). These methods include spectrophotometry , capillary zone electrophoresis (CZE)[7], high performance liquid chromatography (HPLC) , micellar electrokinetic capillary chromatography and thin layer chromatography (TLC) . Square wave and adsorptive anodic stripping voltammetric methods were previously investigated , applying polyaniline/graphene composite modified GCE and boron‐doped diamond electrodes .…”

Zero and second‐derivative synchronous fluorescence spectroscopy for the quantification of two non‐classical β‐lactams in pharmaceutical vials: Application to stability studies

“…The literature survey comprised several analytical methods for quantitative determination of (MP) and (EP). These methods include spectrophotometry, capillary zone electrophoresis (CZE), high performance liquid chromatography (HPLC), micellar electrokinetic capillary chromatography and thin layer chromatography (TLC); square wave and adsorptive anodic stripping voltammetric methods were previously investigated…”

“…[3,4] The literature survey comprised several analytical methods for quantitative determination of (MP) and (EP). These methods include spectrophotometry [5,6] , capillary zone electrophoresis (CZE) [7] , high performance liquid chromatography (HPLC) [8][9][10][11][12][13][14][15][16][17][18][19][20][21][22] , micellar…”