New SNPs in the IGF2 gene and association between this gene and backfat thickness and lean meat content in Large White pigs

Abstract

IGF2-in3-G3072A is a causative mutation for paternally expressed quantitative trait loci on the p arm of porcine chromosome 2 with substantial effect on muscle growth and backfat thickness. The linkage disequilibrium between IGF2-in3-G3072A and IGF2-in7-G162C (IGF2-NciI) in four breeds and associations between these polymorphisms and growth and meat performance in pigs of the Large White breed were analysed. A significant effect of these polymorphisms on backfat thickness and lean meat content was found. In addition, we identified two new single nucleotide polymorphisms (SNPs) in intron 7 of the gene. The existence of complete linkage disequilibrium between IGF2-in3-G3072A locus in the population under study where the locus segregated and SNPs in intron 7 of the IGF2 gene detectable with simple and reliable polymerase chain reaction-restriction fragment length polymorphism techniques (G162C, C179G and G186T) offer possibilities to use these SNPs for genotyping of quantitative trait nucleotide in Large White and Landrace breeds.

Association analysis of seven candidate genes with performance traits in Czech Large White pigs

“…Our results could be influenced by the absence of GG genotype, and by the low frequency of G allele (0.18) in the studied population of Czech Large White. The low frequency of G allele was observed in other studies in Large White (Vykoukalová et al, 2006;Yang et al, 2006;Oczkowicz et al, 2009). Previously reported associations of the IGF2 gene and lean meat content and backfat thickness in the Black Pied Přeštice pig breed (Civáňová and Knoll, 2007) and in Large White (Vykoukalová et al, 2006) were not confirmed by our study, however the IGF2 gene was associated (P ≤ 0.01) with total EBV as the only one of the analysed genes.…”

“…Paternally expressed insulin-like growth factor 2 (IGF2) is one of the polypeptide growth factors which play an important role in foetal growth and development, tumour cell proliferation, and muscle growth; its causative mutation is located in intron 3 of the IGF2 gene (IGF2-int3-3072G>A) and affects muscle development and backfat thickness; A allele is accompanied by higher muscle development and lower fat deposition (Jungerius et al, 2004;Vykoukalová et al, 2006). NAMPT (visfatin) is an essential enzyme in the NAD biosynthetic pathway and current research suggests a role of visfatin in glucose metabolism and the pathogen- esis of type 2 diabetes, it can be associated with subcutaneous or visceral fat, and insulin resistance contributes to abnormal lipid metabolism.…”

An association of C/T polymorphism in exon 2 of the bovine insulin-like growth factor 2 gene with meat production traits in Polish Holstein-Friesian cattle

“…It abrogates the in vitro interaction with a nuclear factor, probably a repressor, and pigs inheriting this mutation from a sire have a threefold increase in the IGF2 messenger RNA expression in postnatal muscle. A significant effect of the porcine IGF2 gene g. 3072A>G polymorphism in intron 3 and g.162C>G (RFLP-NciI) in intron 7 on backfat thickness and lean meat content was found in Large White and Landrace breeds (Vykoukalová et al, 2006). A paternally expressed QTL for muscle growth and backfat thickness has been identified near the IGF2 locus on the distal tip of pig chromosome 2 (SSC2p), and then a mutation in a regulatory element of the IGF2 gene was identified as the quantitative trait nucleotide (QTN) underlying this QTL.…”

MC1R, KIT, IGF2, and NR6A1 as markers for genetic differentiation in Thai native, wild boars, and Duroc and Chinese Meishan pigs

“…Insulin like growth factor-2, which is mapped on pig chromosome 2, has a primary effect on muscle growth and fat deposition. A SNP variation in IGF2, at intron 2 g.3072 A, was highly frequent in Duroc pigs but absent in the Iberian pig (Vykoukalová et al, 2006;Alves et al, 2012). The gene NR6A1, mapped on chromosome 1, determines the number of vertebrae in pigs.…”