An association of C/T polymorphism in exon 2 of the bovine insulin-like growth factor 2 gene with meat production traits in Polish Holstein-Friesian cattle

Abstract

ABSTRACT:The effect of the insulin-like growth factor 2 (IGF2) gene polymorphism -a g.292C>T transition in exon 2 on feed uptake and conversion, growth rates, and meat production traits in Polish HolsteinFriesian cattle was investigated. A total of 291 young bulls were genotyped using restriction fragment length polymorphism (RFLP-BsrI). Animals were slaughtered at the age of 11 or 15 months and carcass traits were examined. At the age of 11 months the association was shown of the CC genotype with higher cold carcass weight, daily gain and meat content (%) in valuable cuts. At 15 months of age the CC genotype bulls showed higher live body weight, whereas those with the CT genotype had more fat in valuable cuts. The TT genotype bulls appeared to consume more feed (dry matter and protein) and used more feed for maintenance and meat production as compared to the CC genotype. The imprinting status of the IGF2 gene was analysed using cDNA sequencing and RFLP-BsrI. In 15-months-old animals and in foetuses older than two months both IGF2 alleles, maternal and paternal, were equally expressed in liver. In a 2-months-old male foetus the IGF2 mRNA was primarily expressed from the paternal allele.

Genetic polymorphisms of the IGF-II gene intron 8 coding region and its association with growth and carcass traits in yak

“…Compared with previous cattle research (Goodall and Schmutz, 2003;Zwierzchowski et al, 2010), the IGF-II gene appears to be highly conserved in yaks, and results indicated a very low mutation rate. It was previously reported that the g.292C>T transition in the IGF-II gene (RFLPBsrI) influenced meat production traits in Polish Holstein-Friesian bulls (Zwierzchowski et al, 2010). In a Canadian beef cattle herd, animals with the CT genotype had a significantly lower mean REA relative to those with the CC genotype, but a significantly higher REA than those with the TT genotype (Goodall and Schmutz, 2007).…”

“…For most traits, the AA and BB genotypes appeared to be favorable, which is consistent with the activity of the IGF-II protein as a growth factor (Heude et al, 2007). However, the observed effects were relatively small, thus suggesting that the mutation is not the primary or causative agent for variation in the traits evaluated (Zwierzchowski et al, 2010). Compared with previous cattle research (Goodall and Schmutz, 2003;Zwierzchowski et al, 2010), the IGF-II gene appears to be highly conserved in yaks, and results indicated a very low mutation rate.…”

“…However, the observed effects were relatively small, thus suggesting that the mutation is not the primary or causative agent for variation in the traits evaluated (Zwierzchowski et al, 2010). Compared with previous cattle research (Goodall and Schmutz, 2003;Zwierzchowski et al, 2010), the IGF-II gene appears to be highly conserved in yaks, and results indicated a very low mutation rate. It was previously reported that the g.292C>T transition in the IGF-II gene (RFLPBsrI) influenced meat production traits in Polish Holstein-Friesian bulls (Zwierzchowski et al, 2010).…”

“…One member of this family, IGF-II, plays a crucial role in muscle growth and differentiation. Therefore, the IGF-II gene is considered to be a candidate molecular marker for selection of favorable meat production traits in farm animals (Zwierzchowski et al, 2010), and has been found to increase in response to degeneration and regeneration, thereby influencing satellite cell activation (Levinovitz et al, 1992;Florini, et al, 1996;Marsh et al, 1997;Bamman et al, 2001;Adams, 2002). Research in pigs has revealed quantitative trait loci (QTL) affecting growth, muscle mass, or fat deposition that map to IGF-I and IGF-II loci (Casas-Carrillo et al, 1997;Nezer et al, 1999;Van Laere et al, 2003).…”

“…Only a few single nucleotide polymorphisms (SNPs) have been found in the bovine IGF-II gene (Zwierzchowski et al, 2010). Curchoe et al (2005) identified an SNP in exon 3 and an SNP after the stop codon of exon 10, and an indel in IGF-II of Bos taurus was reported by Flisikowski et al (2006).…”