MC1R, KIT, IGF2, and NR6A1 as markers for genetic differentiation in Thai native, wild boars, and Duroc and Chinese Meishan pigs

Klomtong, Pitchayanipa; Chaweewan, Kamon; Phasuk, Yupin; Duangjinda, Monchai

doi:10.4238/2015.october.19.16

Cited by 12 publications

(14 citation statements)

References 27 publications

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“…In previous studies, the C allele of NR6A1 has appeared in wild boars and in several indigenous pigs, especially Asian pigs (20,22,29). The T allele frequency in Amami-Shimabuta pig was higher compared to other indigenous Asian pigs (23,29). This indicates that the domestication of Amami-Shimabuta is considered to be more advanced than that of the other indigenous Asian pigs examined on the body elongation to increase meat production.…”

Section: Discussionmentioning

confidence: 88%

NR6A1Allelic Frequencies as an Index for both Miniaturizing and Increasing Pig Body Size

Ijiri

Lai

Kawaguchi

et al. 2021

In Vivo

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Section: Discussionmentioning

confidence: 88%

NR6A1Allelic Frequencies as an Index for both Miniaturizing and Increasing Pig Body Size

Ijiri

Lai

Kawaguchi

et al. 2021

In Vivo

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“…Mislabeling of species in meat products is most commonly detected by DNA analysis 1 , 2 . For the identification of pork, a number of methods based on polymerase chain reaction (PCR)—restriction fragment length polymorphism (RFLP) has been published 3 – 5 . Since PCR–RFLP is laborious and time consuming and not applicable to complex food products, it has mainly been replaced by singleplex 6 – 9 and multiplex real-time PCR assays 10 – 14 .…”

Section: Introductionmentioning

confidence: 99%

Applicability of a duplex and four singleplex real-time PCR assays for the qualitative and quantitative determination of wild boar and domestic pig meat in processed food products

Kaltenbrunner

Mayer

Kerkhoff³

et al. 2020

Sci Rep

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Appropriate analytical methods are needed for the detection of food authentication. We investigated the applicability of a duplex real-time PCR assay targeting chromosome 1 and two singleplex real-time PCR assays targeting chromosome 9, both published recently, for the qualitative and quantitative determination of wild boar and domestic pig in processed food products. In addition, two singleplex real-time PCR assays targeting chromosome 7 were tested for their suitability to differentiate the two subspecies. Even by targeting the three genome loci, the probability of misclassification was not completely eliminated. Application of the real-time PCR assays to a total of 35 commercial meat products, including 22 goulash products, revealed that domestic pig DNA was frequently present, even in 14 out of 15 products declared to consist of 100% wild boar. Quantitative results obtained with the real-time PCR assays for wild boar (p < 0.001) and those for domestic pig (p < 0.001) were significantly different. However, the results obtained with the real-time PCR assays for wild boar (r = 0.673; p < 0.001) and those for domestic pig (r = 0.505; p = 0.002) were found to be significantly correlated. If the rules given in the paper are followed, the real-time PCR assays are applicable for routine analysis.

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“…Another research piece revealed a mutation in the NR6A1 gene of European pigs, resulting in a change in the binding capacity of the NR6A1 protein and inhibitory proteins that interact with the NR6A1 protein [9]. Furthermore, polymorphisms in the NR6A1 gene in Duroc pigs may affect body length [15]. It was confirmed that NR6A1 is prominently associated with the number of vertebrae in Tongcheng (TC) pigs [16].…”

Section: Introductionmentioning

confidence: 99%

“…In addition, the high homology of NR6A1 among different species indicates its potential functional conservation [18]. Based on previous research [8,9,10,11,12,13,14,15,16,17,18], it is rational to hypothesize that the NR6A1 gene may be associated with body size traits of other species. Presently, there is no research regarding the NR6A1 gene in donkeys.…”

Section: Introductionmentioning

confidence: 99%

A Novel 13 bp Deletion within the NR6A1 Gene Is Significantly Associated with Growth Traits in Donkeys

Fang

Lai

Liu

et al. 2019

Animals

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Simple SummaryThe detection of genes potentially associated with economic traits and identification of effective variants can provide a basis for molecular marker-assisted selection of livestock. NR6A1 is a member of the nuclear receptor family and is an important candidate gene related to body size traits. Previous studies showed that NR6A1 gene was associated with body size traits in pigs and other livestock, however, it has not yet been observed in donkeys. In the current study, a 13 bp deletion in NR6A1 gene was firstly identified in donkeys. Analysis showed that this deletion had significant associations with body size traits.AbstractNuclear receptor subfamily 6, group A, member 1 (NR6A1), as an important member of the nuclear receptor family, plays an important role in regulating growth, metabolism, and differentiation of embryonic stem cells. For this reason, the NR6A1 gene is considered to be a promising candidate for economic traits and was found to be associated with body size traits in many livestock. However, no studies have been conducted on NR6A1 in donkeys so far. Thus, in this research, we focused on donkeys and identified a 13 bp deletion in intron-1 of the NR6A1 gene among 408 individuals from Guanzhong and Dezhou donkeys using polyacrylamide gel electrophoresis. Three genotypes were identified, namely II, ID, and DD. The association analysis indicated that the body lengths and body heights5f genotype II individuals were significantly different to those of genotype ID in Dezhou donkeys. Conclusively, the 13 bp deletion was associated with growth traits in both Guanzhong donkeys and Dezhou donkeys, indicating that the NR6A1 gene could be a possible candidate gene in marker-assisted selection for donkey breeding programs.

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MC1R, KIT, IGF2, and NR6A1 as markers for genetic differentiation in Thai native, wild boars, and Duroc and Chinese Meishan pigs

Cited by 12 publications

References 27 publications

NR6A1Allelic Frequencies as an Index for both Miniaturizing and Increasing Pig Body Size

NR6A1Allelic Frequencies as an Index for both Miniaturizing and Increasing Pig Body Size

Applicability of a duplex and four singleplex real-time PCR assays for the qualitative and quantitative determination of wild boar and domestic pig meat in processed food products

A Novel 13 bp Deletion within the NR6A1 Gene Is Significantly Associated with Growth Traits in Donkeys

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