New set of PCR primers for SYBR green-based qPCR detection of IMNV in India

Kokkattunivarthil, Shyam; Krishnan, Rahul; Jeena, K.; Prasad, K. Pani; Naik, T Vismai

doi:10.1016/j.aquaculture.2018.06.061

Cited by 5 publications

(3 citation statements)

References 35 publications

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“…IMNV was first reported in 2003 in P. vannamei cultured in northeastern Brazil, then in Indonesia (2006), and most recently in India (2016), Malaysia (2018) and Indonesia (2018) [ 78 , 79 ] ( Figure 12 ). Until the IMNV virus was reported in India in 2016, it had only occurred in Brazil and Indonesia [ 80 ]. IMNV occurs in P. vannamei, its infectious host, and causes infective myonecrosis.…”

Section: Rna Viral Diseasesmentioning

confidence: 99%

Viral Shrimp Diseases Listed by the OIE: A Review

Lee

Choi

et al. 2022

Viruses

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Shrimp is one of the most valuable aquaculture species globally, and the most internationally traded seafood product. Consequently, shrimp aquaculture practices have received increasing attention due to their high value and levels of demand, and this has contributed to economic growth in many developing countries. The global production of shrimp reached approximately 6.5 million t in 2019 and the shrimp aquaculture industry has consequently become a large-scale operation. However, the expansion of shrimp aquaculture has also been accompanied by various disease outbreaks, leading to large losses in shrimp production. Among the diseases, there are various viral diseases which can cause serious damage when compared to bacterial and fungi-based illness. In addition, new viral diseases occur rapidly, and existing diseases can evolve into new types. To address this, the review presented here will provide information on the DNA and RNA of shrimp viral diseases that have been designated by the World Organization for Animal Health and identify the latest shrimp disease trends.

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Section: Rna Viral Diseasesmentioning

confidence: 99%

Viral Shrimp Diseases Listed by the OIE: A Review

Lee

Choi

et al. 2022

Viruses

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“…[208][209][210][211] To date, multiple PCR-based diagnostic methods have been developed for TSV detection as Table 8. Nunan et al 212 (2018) 200 1 region, respectively. Tang and Lightner 208 found that CP-1 of TSV showed the lowest genetic distance (1.4% nucleotide variances) among 40 TSV isolates derived from Ecuador, Colombia, Honduras, the USA, Mexico, Belize, Thailand, China and Indonesia, and therefore is more conserved than the other regions.…”

Section: Taura Syndrome Virusmentioning

confidence: 99%

“…The TaqMan-based realtime qPCR assay targets an ORF-1 region of IMNV and detects as few as 10 IMNV RNA copies. 201 Although SYBR-Green-based real-time PCR assays have been developed, 199,200 the WOAH-recommended TaqMan-based method 201 is currently most used and considered as a gold standard for IMNV detection together with the nested RT-PCR assay 190 (Table 7).…”

Section: Infectious Myonecrosis Virusmentioning

confidence: 99%

Nucleic acid amplification‐based methods for diagnosis of shrimp viral diseases

Lee,

Vijayan,

Roh

et al. 2023

Reviews in Aquaculture

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Viral diseases are one of the constraints affecting the sustainability of the shrimp farming industry worldwide. The common causes of viral infections include white spot syndrome virus, infectious hypodermal and haematopoietic necrosis virus, hepatopancreatic parvovirus, Penaeus monodon nudivirus, decapod iridescent virus 1, Taura syndrome virus, yellow head virus, infectious myonecrosis virus and Macrobrachium rosenbergii nodavirus. Accurate diagnostic methods are necessary for preventing the spread of these transboundary diseases. Many molecular diagnostic methods for shrimp viral diseases have been developed and are currently in use, including conventional polymerase chain reaction (PCR), nested PCR, loop‐mediated isothermal amplification PCR, recombinase polymerase assay and real‐time PCR. Although the World Organization for Animal Health (founded as OIE) recommends several PCR methods for shrimp disease diagnosis, there has been a lack of efforts to comparatively evaluate the performance of the available methods, resulting in a significant knowledge gap. Therefore, in this review, we compare various PCR assays and commercial PCR‐based diagnostic kits that have been developed and/or published previously, and discuss pros and cons of each of these methods. Our objective of this review is to offer enhanced clarity and a comprehensive overview of molecular diagnostic methods for shrimp viral diseases. This endeavour would serve as an important resource for researchers engaged in relevant studies and professionals within the shrimp‐farming industry, providing valuable guidance and insights.

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