New Probiotic Culture of Lactococcus lactis ssp. lactis: Effective Opportunities and Prospects

Abstract. Bacteriocin-producing strains of Lactococcus lactis subsp. lactis of different origin were used in our experiments. UV-Irradiation of cells of bacteriocin-synthesizing lactoococci of different origin in a dose of 7.6-7600 erg/mm 2 was followed by their selective growth in an MRS medium, and their selection after UV-ray treatment. Some strains of lactococci had double UV treatment. The novel most active bacteriocin-producing strains were restored after lyophilization and storage. An exposure of 10 minutes should be considered an effective dose of treatment for wide strain 229. As a result, No.12 with an antibiotic activity of 3850 IU / mL was selected, which is 54 % higher than the initial one. The study of the physiological and biochemical properties of the variants, selected after UV-ray treatment, showed that they somewhat changed the rate of their growth and the accumulation of bacteriocin. The relative activity in the production of bacteriocin was calculated as the ratio of activity values and the number of produced cells. The inactivated homologous strain biomass and amino acids were applied as the factors of resuscitation. The control culture liquid was without supplements. VBNC cells of opportunistic strains formed within the first days of incubation. After 1 year of incubation, the VBNC values of Klebsiella pneumoniae 1954, Alcaligenes faecalis 415, Enterobacter aerogenes 418, Proteus vulgaris HX19222, Salmonella typhimurium were statistically equal (97.1-99.9%). After 1 day of stress, the strains of L. lactis did not form colonies up to 60-80%, after 5 days up to 82.1-99.6%, and after 1 year -99.9%. Unwashed from the culture fluid, the inoculated cells proliferated and passed to the VBNC faster. With that inoculation, the nisin productive activity of cells was lower 10-78 times, depending on the strain of L. lactis. The study of resuscitation factors has shown that the supplement of homologous inactivated biomass of L. lactis (0.1%, 0.5%, 1%) was effective with 1% (a magnification of 2.65 at p<0.05) and 0.5% (magnification of 3.75 at p<0.05) only for the MSU strain. Strain F-116 was marked by a 4-fold increase in the ability for cultivation after the addition of 7 aminoacids: (glutamine, methionine, leucine, isoleucine, histidine, arginine, valine). After 1 year, the quantitative level of VBNC cells, which formed in the first days of incubation, was the same for opportunistic and probiotic cultures (97.1%-99.9%). All studied resuscitation factors were individually effective for bacterial strains.

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“…It has been revealed, that the strain F-116 produced an antibiotic complex which differed from nisin [19]. UVL "+" variants No.…”

Section: Resultsmentioning

confidence: 99%

Influence of Various Factors on the Lactococcus Lactis Cells

Stoyanova¹,

Блинкова²,

Dbar³

2017

RAD Conference Proceedings

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“…Lactococcus lactis yang mampu menghasilkan asam laktat lebih cepat dibandingkan dengan bateri asam laktat lainnya yang bertugas menekan pertubuhan bakteri patogen seperti Escherichia coli (Nuryhev et al, 2016). Lactobacillus acidophilus merupakan salah satu bahan campuran untuk pembuatan produk fermentasi yang baik untuk kesehatan saluran pencernaan, dan juga rongga mulut.…”

Section: Probiotik Yang Digunakan Adalahunclassified

PENGGUNAAN PROBIOTIK KOMBINASI lactococcus lactis DAN L actobacillus acidophilus SEBAGAI PENGGANTI ANTIBIOTIKA PADA AYAM PETELUR YANG DIINFEKSI E scherichia coli TERHADAP ANALISIS USAHA

Wardhani

Lokapirnasari

Soepranianondo

2019

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Escherichia coli is a normal flora in the digestive system of laying hens that are non-pathogenic, which can change into pathogens and cause the egg production to decrease. So the combination of Lactococcus lactis and Lactobacillus acidophilus probiotics is expected to be able to overcome E. coli and become a substitute for the antibiotics (Virginiamycin) in animal feed. This study aims to determine business analysis including Break Event Points (BEP), Revenue Cost Ratio (R / C Ratio), Payback Period (PP) and Return On Investment (ROI). The best results obtained for the calculation of Break Event Point (BEP) on a0b2 treatment with a BEP of Rp. 17,587,24 with BEP production on a1b2 of 14,36 kg, Revenue Cost Ratio (R / C Ratio) generates a value of 1,543 for treatment a0b2, Payback Period (PP) generates a value of 1 year 3 months 9 days and Return On Investment (ROI) generates a value of 3. It was concluded that the a0b2 treatment had good results to be developed.

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“…OTU5, OTU6, and OTU7 were closely related to Lactococcus lactis. The L. lactis bacteria known to be safe for humans (Laroute et al 2017;Nuryshev et al 2016). L. lactis isolated from milk in Russia has antimicrobial activity against Gram positive and Gram-negative bacteria and antifungal to the fungi of the genus Aspergillus, Fusarium, and Candida (Nuryshev et al, 2016).…”

Section: The Sequence Homology Of Bacteria Based On 16s Rrna Gene Seqmentioning

confidence: 99%

“…The L. lactis bacteria known to be safe for humans (Laroute et al 2017;Nuryshev et al 2016). L. lactis isolated from milk in Russia has antimicrobial activity against Gram positive and Gram-negative bacteria and antifungal to the fungi of the genus Aspergillus, Fusarium, and Candida (Nuryshev et al, 2016). These bacteria are resistant to acidic environments and bile salts, are sensitive to antibiotics, and exhibit treatment effects in mice suffering from chronic ileum-dermatitis (Nuryshev et al 2016).…”

Section: The Sequence Homology Of Bacteria Based On 16s Rrna Gene Seqmentioning

confidence: 99%

“…L. lactis isolated from milk in Russia has antimicrobial activity against Gram positive and Gram-negative bacteria and antifungal to the fungi of the genus Aspergillus, Fusarium, and Candida (Nuryshev et al, 2016). These bacteria are resistant to acidic environments and bile salts, are sensitive to antibiotics, and exhibit treatment effects in mice suffering from chronic ileum-dermatitis (Nuryshev et al 2016). Based on this information, OTU5, OTU6, and OTU7 were predicted to have potential candidates for probiotics with functional properties as antibacterial and antifungal, which may play an important role in the treatment of chronic ileum dermatitis.…”

Section: The Sequence Homology Of Bacteria Based On 16s Rrna Gene Seqmentioning

confidence: 99%

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Characterization of abundance and diversity of lactic acid bacteria from Apis dorsata hives and flowers in East Nusa Tenggara, Indonesia

et al. 2018

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Karyawati AT, Nuraida L, Lestari Y, Meryandini A. 2018. Characterization of abundance and diversity of lactic acid bacteria from Apis dorsata hives and flowers in East Nusa Tenggara, Indonesia. Biodiversitas 19: 899-905. Previous research on lactic acid bacteria associated with honey bees has been conducted in temperate arid regions and wet tropical regions. We isolated lactic acid bacteria from giant honey bee (Apis dorsata) hives and flowers in East Nusa Tenggara, Indonesia, an area with a tropical savanna climate. Diversity of lactic acid bacteria was studied using Denaturing Gradient Gel Electrophoresis method. The purpose of this study was to obtain information about the diversity of lactic acid bacteria contained in Apis dorsata hives and flowers from tropical savanna climate. We identified seven Operational Taxonomy Units (OTU) within the hives and flowers. Two OTUs were closely related to LactoBacillus kunkeei strain YH-15 (T), while three others were closely related to Lactococcus lactis subsp. tructae strain L105 (T). This information is important to explore the potential utilization of lactic acid bacteria in maintaining human health

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New Probiotic Culture of Lactococcus lactis ssp. lactis: Effective Opportunities and Prospects

Cited by 12 publications

References 7 publications

Influence of Various Factors on the Lactococcus Lactis Cells

Influence of Various Factors on the Lactococcus Lactis Cells

PENGGUNAAN PROBIOTIK KOMBINASI lactococcus lactis DAN L actobacillus acidophilus SEBAGAI PENGGANTI ANTIBIOTIKA PADA AYAM PETELUR YANG DIINFEKSI E scherichia coli TERHADAP ANALISIS USAHA

Characterization of abundance and diversity of lactic acid bacteria from Apis dorsata hives and flowers in East Nusa Tenggara, Indonesia

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