New polymorphism in the 5′ flanking region of IGF-1 gene and its association with wool traits in Egyptian Barki sheep

Darwish, Hassan R.; El-Shorbagy, Haidan M.; Abou-Eisha, Ahmed; El-Din, Adel E.; Farag, Ibrahim M.

doi:10.1016/j.jgeb.2017.08.001

Cited by 15 publications

(10 citation statements)

References 23 publications

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“…AF492765.1), three genotypes and two alleles were identified ( Table 2). Similar band patterns were found in the Makooei and Zel sheep (Kazemi et al, 2011), and similar allelic frequencies were reported in the Barki breed (Darwish et al, 2017), with slight changes in genotype frequencies. On the other hand, in Mehraban sheep, the reported frequencies differ significantly (Bahrami et al, 2013) to our results.…”

Section: Pcr-sscp Including A/a C/c A/b A/c A/b/c A/b/e A/b/fsupporting

confidence: 82%

Polymorphisms of the CAPN, CAST, LEP, GH, GHR, IGF-1 and MSTN loci of Colombian Creole hair x Pelibuey sheep sheep crossbreeds

2020

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The objective of this study was to characterize single nucleotide polymorphisms at the Calpain (CAPN), Calpastatin (CAST), Leptin (LEP), Growth Hormone (GH), Growth Hormone Receptor (GHR), Insulin-Like Growth Factor 1 (IGF-1) and myostatin (MSTN) loci in Columbian Creole hair sheep crossed with Pelibuey sheep. In 192 individuals, the CAST and MSTN loci were genotyped by PCR-RFLP, and the CAPN, GH, GHR, IGF-1 and LEP loci by PCR-SSCP and sequences. The following parameters were calculated: allelic and genotypic frequencies, observed (Ho) and expected (He) heterozygosity, fixation index and deviations from Hardy-Weinberg equilibrium (HWE). In the CAPN locus, higher frequencies of the T allele (70%) were found with respect to C (30%) and the frequencies of the TT (46%) and TC (48%) genotypes exceeded the CC (6%) genotype. For the CAST locus, the MM genotype (83%) was the most frequent, followed by the other genotypes (MN 16% and NN 0.5%) and thus the allelic frequencies were M: 91% and N: 9%. The allele A (66%) of the LEP locus was more frequent than the G allele (34%) and the AA, AG and GG genotypes had frequencies of 43, 47 and 10%, respectively. Only two genotypes (AA 64% and AG 36%) were found in the GH locus with allelic frequencies of 82 and 18% for A and G respectively. In the locus IGF-1, the genotypes AA, GG, and AG presented frequencies of 36, 49 and 15%, respectively, with a greater presence of the G allele (56%) than A (44%). In the analysed population, the GHR and MSTN loci were monomorphic, and only the AA and MM genotypes were found in each. The CAST, GH, and IGF-1 loci showed deviations from HWE. He deficit was found only in the IGF-1 locus. The CAPN, CAST, LEP, GH, and IGF-1 loci were polymorphic with high He and excess heterozygotes, in contrast, the GHR and MST loci were monomorphic. The data presented here could be used in animal breeding programmes.

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Section: Pcr-sscp Including A/a C/c A/b A/c A/b/c A/b/e A/b/fsupporting

confidence: 82%

Polymorphisms of the CAPN, CAST, LEP, GH, GHR, IGF-1 and MSTN loci of Colombian Creole hair x Pelibuey sheep sheep crossbreeds

2020

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“…Wu-Jan et al, (2010) and Sharma et al, (2013) also reported significant association of IGF genotype with body weight in cashmere goats and Sirohi goats, respectively. Darwish et al (2017) also revealed G to C transversion at nucleotide position 85 and transition from G to A at position 87 in IGF-1 gene of Egyptian Barki sheep. Similarly, Naicy et al, (2017) reported one SNP at A224G in the 5' non-coding region of the IGF-1 gene that was significantly associated with growth traits in Attappady Black goats.…”

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confidence: 79%

“…Similarly, Naicy et al, (2017) reported one SNP at A224G in the 5' non-coding region of the IGF-1 gene that was significantly associated with growth traits in Attappady Black goats. The association of IGF-I gene polymorphism with economic traits in the 5'-flanking region with body weight, has also been described by Moody et al (1996), Li et al (2004), Moradian et al (2013) and Darwish et al (2017). No association of IGF-1 polymorphism with production traits was reported by Curi et al (2005) in cattle, Wu-Jan et al (2010) in cashmere goats, Rasouli et al, (2017) in Markhoz goats and Sankhyan et al, (2019) in Gaddi goat.…”

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confidence: 86%

Study on Genetic Polymorphism of IGF-1 Gene in Barbari and Jamunapari Goat

Dige¹,

Kaushik²,

Kushwah³

et al. 2020

IJAR

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Insulin like growth factor (IGF 1) play an important role in the production and reproduction. One hundred and ninety two adult goats of Barbari and Jamunapari breed were taken for study. Data on growth traits were recorded to identify SNPs in the IGF-1gene by using High Resolution Melting (HRM) technique. Five fragments of IGF-1 were amplified length of amplicon were 105, 124, 150, 122 and 122 bp covering 5’UTR, 3’UTR, Exon 3, Exon 2 and 5’UTR regions, respectively and subsequently sequenced. The HRM analysis revealed no sequence variation among the samples of both breeds. Hence, the all five amplified fragments of IGF-1 gene showed monomorphic pattern and, no significant association could be detected with growth traits in Barbari and Jamunapari goats.

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“…Insulin-like Growth Factor Binding Proteins (IGFBPs) belongs to a family of at least six homologous proteins that bind IGFs and modulate many of their important biological actions [4]. It is recommended as a marker for different body functions such as growth, body weight, reproduction, immunity, metabolism, and energy balance [5][6][7][8]. IGFBP-3 gene is responsible for the multiple and necessary effects of (IGF) [3][4][5].…”

Section: Introductionmentioning

confidence: 99%

Comparative analysis of IGFBP-3 gene sequence in Egyptian sheep, cattle, and buffalo

Saleh

Rashad

Hassanine

et al. 2019

Preprint

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Objective: A total of 205 animals from four Egyptian livestock species; cattle (n=18), buffaloes (n=12), sheep (n=150) and goats (n=25) were used in this study to detect polymorphism and perform comparative analysis for IGFBP-3 gene using DNA sequencing and (PCR-RFLP). Results: The amplified fragments were found to be of length 654 bp in sheep, 651bp in cattle and 655 bp in buffalo. For Falahy goats, PCR was performed to amplify a 316 bp fragment from exon 2 of the IGFBP-3 gene. The digestion of 654 bp with HaeIII restriction enzyme yielded a single restriction pattern for goats, while for cattle, 3 genotypes were identified; (AA),(AB), and (BB). Moreover, for buffalo one genotype (AA) only was found with HaeIII and TaqI restriction enzymes, separately. Also, the digestion profile for goats with HaeIII revealed one pattern only. Nucleotide sequencing of the amplified fragments of IGFBP-3 gene in sheep, cattle, buffalo, and goat was submitted to the NCBI GenBank (Accession no. MG738671.1, MG738673.1, MG738674.1, and MG738672.1, respectively). The nucleotide sequencing analysis indicated similarity percentages in IGFBP-3 gene fragments of 88.54, 89.63 and 95.06 % between ''sheep and cattle '', ''sheep and buffalo '', and ''cattle and buffalo'', respectively.

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New polymorphism in the 5′ flanking region of IGF-1 gene and its association with wool traits in Egyptian Barki sheep

Cited by 15 publications

References 23 publications

Polymorphisms of the CAPN, CAST, LEP, GH, GHR, IGF-1 and MSTN loci of Colombian Creole hair x Pelibuey sheep sheep crossbreeds

Polymorphisms of the CAPN, CAST, LEP, GH, GHR, IGF-1 and MSTN loci of Colombian Creole hair x Pelibuey sheep sheep crossbreeds

Study on Genetic Polymorphism of IGF-1 Gene in Barbari and Jamunapari Goat

Comparative analysis of IGFBP-3 gene sequence in Egyptian sheep, cattle, and buffalo

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