Objective: A total of 205 animals from four Egyptian livestock species; cattle (n=18), buffaloes (n=12), sheep (n=150) and goats (n=25) were used in this study to detect polymorphism and perform comparative analysis for IGFBP-3 gene using DNA sequencing and (PCR-RFLP). Results: The amplified fragments were found to be of length 654 bp in sheep, 651bp in cattle and 655 bp in buffalo. For Falahy goats, PCR was performed to amplify a 316 bp fragment from exon 2 of the IGFBP-3 gene. The digestion of 654 bp with HaeIII restriction enzyme yielded a single restriction pattern for goats, while for cattle, 3 genotypes were identified; (AA),(AB), and (BB). Moreover, for buffalo one genotype (AA) only was found with HaeIII and TaqI restriction enzymes, separately. Also, the digestion profile for goats with HaeIII revealed one pattern only. Nucleotide sequencing of the amplified fragments of IGFBP-3 gene in sheep, cattle, buffalo, and goat was submitted to the NCBI GenBank (Accession no. MG738671.1, MG738673.1, MG738674.1, and MG738672.1, respectively). The nucleotide sequencing analysis indicated similarity percentages in IGFBP-3 gene fragments of 88.54, 89.63 and 95.06 % between ''sheep and cattle '', ''sheep and buffalo '', and ''cattle and buffalo'', respectively.

Objective: A total of 205 animals from four Egyptian livestock species; cattle (n=18), buffaloes (n=12), sheep (n=150) and goats (n=25) were used in this study to detect polymorphism and perform comparative analysis for IGFBP-3 gene using DNA sequencing and (PCR-RFLP). Results: The amplified fragments were found to be of length 654 bp in sheep, 651bp in cattle and 655 bp in buffalo. For Falahy goats, PCR was performed to amplify a 316 bp fragment from exon 2 of the IGFBP-3 gene. The digestion of 654 bp with HaeIII restriction enzyme yielded a single restriction pattern for goats, while for cattle, 3 genotypes were identified; (AA),(AB), and (BB). Moreover, for buffalo one genotype (AA) only was found with HaeIII and TaqI restriction enzymes, separately. Also, the digestion profile for goats with HaeIII revealed one pattern only. Nucleotide sequencing of the amplified fragments of IGFBP-3 gene in sheep, cattle, buffalo, and goat was submitted to the NCBI GenBank (Accession no. MG738671.1, MG738673.1, MG738674.1, and MG738672.1, respectively). The nucleotide sequencing analysis indicated similarity percentages in IGFBP-3 gene fragments of 88.54, 89.63 and 95.06 % between ''sheep and cattle '', ''sheep and buffalo '', and ''cattle and buffalo'', respectively.

Objective: A total of 205 animals from four Egyptian livestock species were used in this study to detect polymorphism and perform comparative analysis for IGFBP-3 gene using DNA sequencing and (PCR-RFLP). Results: The digestion of 654 bp with HaeIII restriction enzyme yielded a single restriction pattern of eight fragments in sheep revealing the absence of polymorphism, while in cattle 3 genotypes were identified; (AA), (AB), and (BB). Moreover, one genotype (AA) only was found in buffalo using HeaIII and MspI restriction enzymes, separately. Digestion profile for a goat with HaeIII revealed only one pattern for three DNA fragments, which means the absence of polymorphism within the IGFBP-3 gene in the tested goats. The nucleotide sequencing analysis indicated similarity percentages in IGFBP-3 gene fragments of 77, 30 and 58% between ''sheep and cattle '', ''sheep and buffalo '', and ''cattle and buffalo'', respectively.

Objective: A total of 205 animals from four Egyptian livestock species; cattle (n=18), buffaloes (n=12), sheep (n=150) and goats (n=25) were used in this study to detect polymorphism and perform comparative analysis for IGFBP-3 gene using DNA sequencing and (PCR-RFLP). Results: The amplified fragments were found to be of length 654 bp in sheep, 651bp in cattle and 655 bp in buffalo. For Falahy goats, PCR was performed to amplify a 316 bp fragment from exon 2 of the IGFBP-3 gene. The digestion of 654 bp with HaeIII restriction enzyme yielded a single restriction pattern for goats, while for cattle, 3 genotypes were identified; (AA),(AB), and (BB). Moreover, for buffalo one genotype (AA) only was found with HaeIII and TaqI restriction enzymes, separately. Also, the digestion profile for goats with HaeIII revealed one pattern only. Nucleotide sequencing of the amplified fragments of IGFBP-3 gene in sheep, cattle, buffalo, and goat was submitted to the NCBI GenBank (Accession no. MG738671.1, MG738673.1, MG738674.1, and MG738672.1, respectively). The nucleotide sequencing analysis indicated similarity percentages in IGFBP-3 gene fragments of 88.54, 89.63 and 95.06 % between ''sheep and cattle '', ''sheep and buffalo '', and ''cattle and buffalo'', respectively.