New nitro-benzo[c]phenanthridine and indolopyridoquinazoline alkaloids from Zanthoxylum atchoum

Yao-Kouassi, Philomène Akoua; Caron, Catherine; Ramiarantsoa, Harisolo; Prost, Élise; Harakat, Dominique; Magrex-Debar, Elisabeth Le; Gangloff, Sophie C.; Coffy, Antoine Ahibo; Zèches-Hanrot, Monique

doi:10.1016/j.crci.2015.01.005

Cited by 10 publications

(4 citation statements)

References 23 publications

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“…Andaliman has been used as spices at North Sumatera especially at North Tapanuli [5], [6], [7]. The plants from Zanthoxylum genus contain many compounds such as phenol hydroquinones, flavonoids, steroids/ triterpenoids, tannins, glycosides, volatile oils, alkaloids, coumarines, lignans, amides and terpenes [8], [9], [10], [11], [12], [13], [14], [15]. Ethyl acetate extract of andaliman fruits (EEA) was showed to have cytotoxicity effect against MCF-7 and T47D cell lines.…”

Section: Introductionmentioning

confidence: 99%

Cell Cycle Inhibition of Ethylacetate Fraction of Zanthoxylum Acanthopodium DC. Fruit against T47D Cells

Satria¹,

Silalahi²,

Haro³

et al. 2019

Open Access Maced J Med Sci

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BACKGROUND: The use of medicinal plants is increasing in several decades for relief many diseases. Indonesia consists of thousands of islands with various plants and the manners of the community using plants as a treatment for every disease traditionally. AIM: Cytotoxic activity of ethyl acetate fraction (EAF) of Zanthoxylum acanthopodium fruit was tested towards T47D breast cancer cells. METHODS: The in vitro cytotoxic activity was performed by MTT assay, and the result was expressed as the IC50 (Inhibitory Concentration), and cell cycle inhibition was investigated by flow cytometry to assess the inhibition in every phase of cell cycle, and the role of expression cyclin D1 and p53 in cell cycle inhibition were performed by immunocytochemistry. RESULTS: EAF was showed to have high activity with a value of IC50 48.94 ± 0.32 µg/mL. EAF of 25 µg/mL caused cell accumulation at G0/G1 (60.48%) and in a control cell (51.69%) and decreased expression of cyclin D1 and increased expression of p53. CONCLUSION: The results obtained in this study provided scientific support for further investigation on compounds in Z. acanthopodium fruit which in the future could be used for medication.

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Section: Introductionmentioning

confidence: 99%

Cell Cycle Inhibition of Ethylacetate Fraction of Zanthoxylum Acanthopodium DC. Fruit against T47D Cells

Satria¹,

Silalahi²,

Haro³

et al. 2019

Open Access Maced J Med Sci

View full text Add to dashboard Cite

show abstract

“…Andaliman has been used as spices at North Sumatera especially at North Tapanuli [5], [6], [7]. The plants from Zanthoxylum genus contain many compounds such as phenol hydroquinones, flavonoids, steroids/triterpenoids, tannins, glycosides, volatile oils, alkaloids, coumarines, lignans, amides and terpenes [8], [9], [10], [11], [12], [13], [14], [15]. Ethylacetate extract of andaliman fruits (EEA) was showed to have cytotoxicity effect against MCF-7 and T47D cell lines.…”

Section: Introductionmentioning

confidence: 99%

Cytotoxicity Activity of Ethanol Extract of Andaliman Fruits (Zanthoxylum acanthopodium DC.) towards 4T1 Breast Cancer Cells

Rosidah

Hasibuan

Haro

et al. 2019

idjpcr

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Breast cancer is one of the world's leading cause of death in women. Due to the resistance of chemotherapeutic agents, there is a continuous need to search of natural products with anticancer activity. The use of natural products is expected to increase the effectiveness and decrease side effect. The purpose of this study was to investigate the anticancer activity of ethanol extract of andaliman fruits (EEAF) towards 4T1 cells. Extracts were prepared by maceration using solvent ethanol 96%. 4T1 cells were grown in culture medium DMEM then given by EEAF and doxorubicin. Cytotoxic test in vitro was done by MTT method [3-(4,5-dimetiltiazol-2-il) -2.5 difeniltetrazolium bromide] which is then analyzed using SPSS 21. The results from this study showed that the cytotoxic results (IC50) after treatment with EEAF and doxorubicin were 54.48 ± 0.22 µg/mL dan 0.80 ± 0.02 µg/mL.Based on the result above, we conclude that EEAF has cytotoxic activity towards 4T1 cancer cells. Key words: andaliman fruits, Zanthoxylum acanthopodium DC., ethanol extract, breast cancer, 4T1 cell line.

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“…Andaliman has been used as spices at North Sumatera, especially at North Tapanuli [6], [7], [8]. The plants from Zanthoxylum genus contain many compounds such as phenol hydroquinones, flavonoids, steroids / triterpenoids, tannins, glycosides, volatile oils, alkaloids, coumarins, lignans, amides and terpenes [9], [10], [11], [12], [13], [14], [15], [16]. Ethylacetate extract of Zanthoxylum acanthopodium DC.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of Cytotoxic Activity Alkaloid Fractions of Zanthoxylum acanthopodium DC. Fruits

Syari¹,

Rosidah²,

Hasibuan³

et al. 2019

Open Access Maced J Med Sci

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AIM: This study was carried out to investigate cytotoxic activity towards T47D, 4T1, MCF-7, HeLa, and Raji cells of alkaloid fractions of Zanthoxylum acanthopodium DC. fruits. Zanthoxylum acanthopodium DC. METHODS: The fruit was extracted by maceration. The ethanol extract was fractionated with liquid-liquid extraction using n-hexane, chloroform at pH 3,7, and 9 to obtained alkaloid fractions. Cytotoxic activity for fraction chloroform at pH 7 and 9 was determined with MTT assay. RESULTS: The IC50 of fraction chloroform at pH 7 and 9 was (92.67 ± 1.37; 71.87 ± 1.04; 159.87 ± 0.63; 123.39 ± 0.81; and 103.09 ± 0.58 µg/mLfor pH 7) and (451.29 ± 25.48; 247.18 ± 2.82; 318.46 ± 5.40; 303.96 ± 8.75; and 181.45 ± 1.35 µg/mL for pH 9) respectively. CONCLUSION: The results reveal that alkaloid fractions at pH 7 and 9 of Zanthoxylum acanthopodium DC. Fruits have cytotoxic activity. Our further study is to isolate and assesses anticancer activity from alkaloid compounds.

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New nitro-benzo[c]phenanthridine and indolopyridoquinazoline alkaloids from Zanthoxylum atchoum

Cited by 10 publications

References 23 publications

Cell Cycle Inhibition of Ethylacetate Fraction of Zanthoxylum Acanthopodium DC. Fruit against T47D Cells

Cell Cycle Inhibition of Ethylacetate Fraction of Zanthoxylum Acanthopodium DC. Fruit against T47D Cells

Cytotoxicity Activity of Ethanol Extract of Andaliman Fruits (Zanthoxylum acanthopodium DC.) towards 4T1 Breast Cancer Cells

Evaluation of Cytotoxic Activity Alkaloid Fractions of Zanthoxylum acanthopodium DC. Fruits

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