Cell Cycle Inhibition of Ethylacetate Fraction of Zanthoxylum Acanthopodium DC. Fruit against T47D Cells

Satria, Denny; Silalahi, Jansen; Haro, Ginda; Ilyas, Syafruddin; Hasibuan, Poppy Anjelisa Zaitun

doi:10.3889/oamjms.2019.178

Cited by 17 publications

(12 citation statements)

References 18 publications

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“…A concentration of 25 mg/mL is likely a safe concentration of Z. acanthopodium extract as an oral anti-inflammatory agent. The previous study reported that the IC50 of the Zanthoxylum acanthopodium extract was 48.94±0.32 µg/mL to T47D breast cancer cell lines 24 . Different solvents might affect the cytotoxicity effect of the extract; Satria et al's study used ethyl acetate, while our study used ethanol as solvent 24 .…”

Section: Discussionmentioning

confidence: 94%

Indonesian andaliman fruit (Zanthoxylum acanthopodium DC.) extract suppresses the expression of inflammatory mediators on fibroblasts cells in vitro

Lelyana¹,

Widyarman²,

Amtha³

2021

Pharm Sci Asia

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The recurrent aphthous stomatitis (RAS) is a common disease of the oral mucosa, manifested by recurrent mucosal ulcers 1,2 . The etiology of RAS is still unclear, but the predisposing factors include trauma, microbial infection, indigestion, blood disorders, emotional disorders (e.g., stress and worry), immunological disorders, nutritional deficiency, genetic disorders, allergy, and hormones (e.g., menstruation cycle) 3,4 . The etiopathogenetic basis is an inflammation due to Streptococcus sanguinis; this organism is one of the normal flora in the oral cavity but can cause an antigenic reaction and recurrent ulcers on the oral mucosa. Immunological changes are an underlying etiology of the oral mucosa inflammation, and the tumor necrosis factor α (TNF-α) and interleukin-6 (IL-6) cytokines play a key role in ulcer progression of oral cavity 4,5 .The primary strategy for RAS treatment is to control the inflammation, to suppress the inflammationassociated cells' activity, reducing the pain of the lesion, and accelerating the healing process 3 . The most commonly used anti-inflammatory agents to treat oral cavity inflammations are topical corticosteroids 6,7 . However, the Indonesian government is currently promoting the use of herbal medicines as alternative treatments

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Section: Discussionmentioning

confidence: 94%

Indonesian andaliman fruit (Zanthoxylum acanthopodium DC.) extract suppresses the expression of inflammatory mediators on fibroblasts cells in vitro

Lelyana¹,

Widyarman²,

Amtha³

2021

Pharm Sci Asia

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“…Previous in vitro studies indicate that some of the compounds isolated from the genus Zanthoxylum exert their anticancer activity through induction of apoptosis and cell cycle arrest. 33,34 Based on the results of our in vitro antiproliferative activities, it was inferred that some of the isolated compounds reported herein inhibit cancer cell proliferation by causing cell cycle arrest and apoptosis. In that regard, we attempted to elucidate the probable anticancer mechanism of action of the isolated compounds against protein targets in the p53 pathway.…”

Section: In Silico Docking Studiesmentioning

confidence: 87%

Antiproliferative Activity of Secondary Metabolites from Zanthoxylum zanthoxyloides Lam: In vitro and in silico Studies

Andima¹,

Coghi²,

Yang³

et al. 2019

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Background: Plant derived compounds have provided proming leads in search for safer anticancer chemotherapies. Zanthoxylum zanthoxyloides is a common medicinal plant in Uganda whose bioactive composition has not been fully elucidated. The aim of this study was to evaluate the in vitro antiproliferative potential of compounds isolated from Zanthoxylum zanthoxyloides and their probable in silico anticancer mechanisms of action. Methods: Column chromatography was used to isolate compounds from MeOH:CH 2 Cl 2 (1:1) extract of the stem bark extract of Zanthoxylum zanthoxyloides. The structures of the isolated compounds were elucidated by NMR and MS analyses. MTT assay was used to measure cell viability. Using in silico docking, the interaction of the compounds with key target proteins in the p53 pathway was determined. Results: From the root bark of this plant five compounds were isolated, namely; dihydrochelerythrine (1), skimmianine (2), tridecan-2-one (3), sesamin (4) and hesperidin (5). Dihydrochelerythrine (1) inhibited proliferation of liver cancer (HCC) cells (IC 50 21.2), breast cancer (BT549) cells, (IC 50 21.2 μM). Similarly, sesamin (4) exhibited moderate inhibitory activity against BT549 cancer cells (IC 50 47.6 μM). Hesperidin (5) showed low inhibitory activity against A549 and HEp 2 (Larynx) cells but was significantly toxic to normal liver and lung cells.In silico docking studies showed that all the compounds strongly bind to cyclin-dependent kinases (CDK2 and CDK6) and weakly bind to caspases 3 and 8 suggesting that they inhibit cancer cells by inducing cell cycle arrest and apoptosis. Conclusion: This study indicates that the compounds isolated from Z. zanthoxyloids hold promise as potential leads against cancer. Due to high toxicity of compound 5 against normal lung and liver cells, it deserves further toxicity investigations to access its safety before in vivo trials.

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“…Nanoherbal of ZA has high antioxidant and had extremely small-sized at 0.440 μm and based on particle size analysis, it has an average diameter of distribution of 783.9 ± 173.4 nm ( Situmorang et al, 2019a ). The cytotoxicity activities in this plant can through cell cycle arrest, reducing cyclin D1, and increasing the expression of p53 ( Satria et al, 2019 ). However, an n-hexane extract from ZA fruit given during the postimplantation period of 6–14 days had a negative effect on mice liver changed the colour and texture of the liver surface and increased damage to hepatocytes ( Sabri et al, 2018 ).…”

Section: Discussionmentioning

confidence: 99%

Histological changes in placental rat apoptosis via FasL and cytochrome c by the nano-herbal Zanthoxylum acanthopodium

Situmorang

Hutahaean

Rosidah

2021

Saudi Journal of Biological Sciences

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Highlights The administration of nanoherbal andaliman reduced apoptosis via cytochrome c and FasL. EVOO reduces apoptosis via cytochrome c and FasL better than andaliman. Combination Nano herbal andaliman and EVOO reduce reduced apoptosis via cytochrome c and FasL on placental histology of hypertension rats. Combination Nano herbal andaliman and EVOO reduce MDA levels in hypertension rats. Nano herbal andaliman and combined with EVOO increase HSP-70 expression in hypertension rats.

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Cell Cycle Inhibition of Ethylacetate Fraction of Zanthoxylum Acanthopodium DC. Fruit against T47D Cells

Cited by 17 publications

References 18 publications

Indonesian andaliman fruit (Zanthoxylum acanthopodium DC.) extract suppresses the expression of inflammatory mediators on fibroblasts cells in vitro

Indonesian andaliman fruit (Zanthoxylum acanthopodium DC.) extract suppresses the expression of inflammatory mediators on fibroblasts cells in vitro

Antiproliferative Activity of Secondary Metabolites from Zanthoxylum zanthoxyloides Lam: In vitro and in silico Studies

Histological changes in placental rat apoptosis via FasL and cytochrome c by the nano-herbal Zanthoxylum acanthopodium

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