New isocoumarins, naphthoquinones, and a cleistanthane-type diterpene from Nectria pseudotrichia 120-1NP

Metabolites from Clonostachys Fungi and Their Biological Activities

“…The polyketides, their isolated Clonostachys fungi and biological activities are shown in Table 2. Antibacterial and phytotoxic activities [50] Nectria pseudotrichia Cytotoxic activity [38] O-Methylherbarin (123) Nectria pseudotrichia 120-1NP Cytotoxic activity [50] Dehydroherbarin (124) Nectria pseudotrichia Cytotoxi activity [38] 2-Acetoxyl-5,7-dimethoxy-3-methyl -1,4-naphthoquinone (125) Nectria pseudotrichia Cytotoxic activity [38] Pseudonectrin A (126) Nectria pseudotrichia Cytotoxic activity [38] Pseudonectrin B (127) Nectria pseudotrichia Cytotoxic activity [38] Pseudonectrin C (128) Nectria pseudotrichia Cytotoxic activity [38] Pseudonectrin D (129) Nectria pseudotrichia - [38] Nectriafurone (130) Nectria haematococca - [52] Nectriaquinone A (131) Nectria pseudotrichia 120-1NP Cytotoxic activity [50] Nectriaquinone…”

“…Herbarin (122) and nectriaquinone B (132) isolated from the brown rice culture of Nectria pseudotrichia 120-1NP exhibited antibacterial activities against Staphylococcus aureus and Pseudomonas aeruginosa [50]. Herbarin (122), O-methylherbarin (123), nectriaquinone A (131), and nectriaquinone B (132) displayed cytotoxic activity against human promyelocytic leukemia HL60 cells with IC50 values of 11.9, 1.33, 1.93, and 11.6 μM, respectively.…”

“…Herbarin (122), O-methylherbarin (123), nectriaquinone A (131), and nectriaquinone B (132) displayed cytotoxic activity against human promyelocytic leukemia HL60 cells with IC50 values of 11.9, 1.33, 1.93, and 11.6 μM, respectively. The structure-function relationship elucidated that the higher cytotoxicity of herbarin (122) and nectriaquinone B (132), compared to that of the related compounds O-methylherbarin (123) and nectriaquinone A (131) was attributed to their increased cell membrane permeability due to the presence of the hydroxyl group [38,50]. In addition, herbarin (122) showed a significant inhibition on lettuce seedling growth [50].…”

Phytotoxic Diterpenoids from Plants and Microorganisms

“…Zythiostromic acid C (160; Figure 3) was isolated from the brown rice cultures of Nectria pseudotrichia 120 -1NP. [40] The phytotoxicity of 160 was assayed by measuring the length of roots and shoots of L. sativa seedlings. 2,4-D was used as the positive control with an IC 50 value of 2.67 μM.…”

Pseudonectrins A–D, heptaketides from an endophytic fungus Nectria pseudotrichia

“…10,[13][14][15][16][17][18] While Nectria pseudotrichia, usually considered as a plant pathogen, has been reported to produce naphthoquinones, isocoumarins, and terpenoids. [19][20][21] During our continuous search for new cytotoxic metabolites from the plant endophytic fungi, [22][23][24][25][26][27][28][29] a strain of Nectria pseudotrichia, isolated from the twigs of an identied tree on Tiantangzhai Mountain, Anhui, People's Republic of China, was grown in a solid-substrate fermentation culture. An ethyl acetate (EtOAc) extract of the culture showed cytotoxic effects towards a small panel of four human tumor cell lines.…”