New insights in molecular mechanisms involved in chronic kidney disease using high-resolution plasma proteome analysis

Glorieux, Griet; Mullen, William; Duranton, Flore; Filip, Szymon K.; Gayrard, Nathalie; Husi, Holger; Schepers, Eva; Neirynck, Nathalie; Schanstra, Joost P.; Jankowski, Joachim; Mischak, Harald; Argilés, Àngel; Vanholder, Raymond; Vlahou, Antonia; Klein, Julie

doi:10.1093/ndt/gfv254

Cited by 65 publications

(58 citation statements)

References 44 publications

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“…Proteomics represents a promising analytical tool to identify blood‐based biomarkers associated with the full spectrum of GFR. However, the studies using proteomic technologies to date have been limited in their clinical application because they were tested in small, overly homogenous cohorts, and lacked validation from iohexol clearance as an independent standard . Furthermore, most proteomics methodologies rely upon MS, which has shown limited reproducibility and specificity for biomarker development in CKD …”

Section: Introductionmentioning

confidence: 99%

The Impact of the Glomerular Filtration Rate on the Human Plasma Proteome

Christensson

Ash

DeLisle

et al. 2018

Proteomics Clinical Apps

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Section: Introductionmentioning

confidence: 99%

The Impact of the Glomerular Filtration Rate on the Human Plasma Proteome

Christensson

Ash

DeLisle

et al. 2018

Proteomics Clinical Apps

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“…5 In addition to oxidative stress and inflammatory process, recent study showed that next to the changes in the expression of coagulation factors and plasma kallikrein, protein related to blood coagulation and platelet activation were also played important roles in the progression of CKD. 6 Inhibition of progression of CKD is important. Several animal studies used active compounds that acted as antioxidants, anti-inflammatory, or antifibrosis to inhibit the progression of CKD.…”

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confidence: 99%

The effects of quercetin on oxidative stress and fibrosis markers in chronic kidney disease rat model

et al. 2017

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ABSTRAK ABSTRACTBackground: Oxidative stress may play a role in the pathogenesis of (CKD), Nuclear factor erythroid 2-related factor 2 (Nrf2) is a transcription factor involved in cell defense mechanism against oxidative stress. In this study, we examined the effect of quercetin, a polyphenplic antioxidant anti fibrosis compund in fruits and vegetables, on the 5/6 nephrectomy-induced CKD progression model rats through modulation of Nrf2 expression.

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“…S1, Supporting Information). Importantly, 15 proteins that were removed to a higher extent by the MCO‐CI dialyzer were previously reported as over‐represented in plasma samples from CKD5 patients undergoing hemodialysis vs. CKD2/3 . The most promising findings are listed in Table .…”

Section: Resultsmentioning

confidence: 97%

Removal of Cell‐Activating Substances Using Dialyzers With Various Permeability Profiles

et al. 2017

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Despite multiple efforts to target an improvement in clinical outcomes of patients with end-stage renal disease, several challenges must still be addressed. Dialysis patients are at a high risk for complications, as reflected by increasing mortality rates. The objective of this study is to assess the impact of the application of dialyzers with varying permeability profiles on the removal of cell-activating substances from the blood of hemodialysis (HD) patients. Dialysate samples were collected using Revaclear 400 (RC) and MCO-Ci400 (MCO-CI). Total protein and solute marker concentrations were determined for the concentrated sample. The response of tubular epithelial cells (TECs) to the dialysate samples was assessed via measurement of interleukin 6, cell viability, and morphology. Proteomic analysis of the dialysate samples was performed using liquid chromatography coupled to tandem mass spectrometry. Treatment of TECs with the MCO-CI dialysate resulted in significantly decreased cell viability compared with the RC dialysate. TECs incubated with samples from MCO-CI lost their typical brick-like shape and cell-cell connections. Proteomic analysis of dialysate samples indicated multiple pro-apoptotic and pro-inflammatory proteins, supporting the observed phenotype. Additionally, application of the MCO-CI dialyzer allowed for more efficient removal of proteins associated with advanced chronic kidney disease stages. Collectively, the use of dialyzer with a higher permeability profile enabled more efficient removal of cell-activating and toxic substances from the blood of HD patients. However, a further large-scale study is needed to address benefits and associated risks for patients.

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New insights in molecular mechanisms involved in chronic kidney disease using high-resolution plasma proteome analysis

Cited by 65 publications

References 44 publications

The Impact of the Glomerular Filtration Rate on the Human Plasma Proteome

The Impact of the Glomerular Filtration Rate on the Human Plasma Proteome

The effects of quercetin on oxidative stress and fibrosis markers in chronic kidney disease rat model

Removal of Cell‐Activating Substances Using Dialyzers With Various Permeability Profiles

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