New immunological assays for the diagnosis of Helicobacter pylori infection

Vaira, Dino; Holton, John; Menegatti, Marcello; Ricci, Chiara; Landi, Fabiana; Alı̀, Anna; Gatta, Luigi; Acciardi, Carmela; Farinelli, S.; Crosatti, Marialuisa; Berardi, Sonia; Miglioli, M.

doi:10.1136/gut.45.2008.i23

Cited by 33 publications

(17 citation statements)

References 37 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Several types of immunological test kits for the detection of H. pylori infection are commercially available, and they showed similar results. 34 Our previous report, 7 in which we could not find any relationship between H. pylori infection and gastric cancer in Korean people, was based on the rapid urease test and histology, so our previous result could be attributed to the underdetection of H. pylori infection, because the bacteria do not colonize areas of severe atrophic gastritis, intestinal metaplasia, 35 and cancer. 36 To evaluate the association between H. pylori infection and gastric cancer, it is important to test for both past infection and present ongoing infection.…”

Section: Discussionmentioning

confidence: 61%

Association between Helicobacter pylori infection and the risk of gastric cancer in the Korean population: prospective case-controlled study

Chang

Kim

et al. 2001

Journal of Gastroenterology

View full text Add to dashboard Cite

show abstract

Section: Discussionmentioning

confidence: 61%

Association between Helicobacter pylori infection and the risk of gastric cancer in the Korean population: prospective case-controlled study

Chang

Kim

et al. 2001

Journal of Gastroenterology

View full text Add to dashboard Cite

show abstract

“…A number of inexpensive, widely available, and reliable serological tests [1,2,3,4] may be used in this setting. In addition, rapid serological whole-blood tests for bedside diagnosis of Helicobacter pylori infection have been developed [5,6,7].…”

Section: Introductionmentioning

confidence: 99%

Serologic Diagnosis of Helicobacter pylori Infection in Outpatients Aged 45 Years or Less

Oksanen

Sipponen

Sarna

et al. 2001

Eur J Clin Microbiol Infect Dis

View full text Add to dashboard Cite

The diagnostic accuracy of serological tests for Helicobacter pylori was studied in 145 consecutive outpatients aged 45 years or less referred for gastroscopy. Helicobacter pylori infection can be detected by serological tests, including rapid whole-blood tests. The low prevalence of the disease in young people may have a negative effect on the positive predictive value of a test. In this study, the presence of Helicobacter pylori was assessed by a biopsy urease test and histological examination, and by several serological tests: a rapid whole-blood test on fingerstick blood, a latex agglutination serum test, a commercial enzyme immunoassay (EIA) test, and an in-house EIA for detection of antibodies of both the IgG and IgA classes. Helicobacter pylori infection was diagnosed with invasive tests in 21 (14.5%) patients. The sensitivity, specificity, and positive and negative predictive values of the EIA-based tests, compared to histological examination, were 100%, 96-97%, 81-84%, and 100%, respectively. The positive predictive value of the latex agglutination test was 78%, whereas it was only 47% for the whole-blood rapid test used. Although the results of the whole-blood rapid test were unsatisfactory, the quantitative EIA-based tests could reliably detect Helicobacter pylori among young patients, in whom the prevalence of the infection is low.

show abstract

“…Current serological tests are unable to distinguish active from past infection. 2 All patients over 45 and those with "alarm" symptoms of weight loss or bleeding require an endoscopy to exclude gastric malignancy, and require an invasive test to be performed. 3 Non-invasive tests are useful for primary diagnosis, when a treatment indication already exists, or to monitor treatment success or failure.…”

mentioning

confidence: 99%