New Fluorescent Method (PLT-F) on Sysmex XN2000 Hematology Analyzer Achieved Higher Accuracy in Low Platelet Counting

Abstract

The new PLT-F method demonstrated excellent results for reproducibility in samples with platelet counts less than 50 × 10(9)/L. PLT-F could be helpful in making better decisions for platelet transfusions.

The Sysmex XN-2000 Hematology Autoanalyzer Provides a Highly Accurate Platelet Count than the Former Sysmex XE-2100 System Based on Comparison with the CD41/CD61 Immunoplatelet Reference Method of Flow Cytometry

“…These results indicate that a more accurate estimation of platelet counts can be achieved using the XN-2000 PLT-F method, with a reduction of bias (0.7×10 9 /L) than using the XE-2100 PLT-O method, as measured by comparison with the CD41/CD61 IRM of flow cytometry. These results support the conclusion of a previous study which reported that the XN-2000 PLT-F method could be more useful than the XN-2000 PLT-O method for accurate platelet counting in thrombocytopenic samples (N=37, platelet counts <50.0×10 9 /L) [1]. In the present study, platelet counts measured using the XN-2000 PLT-F method were compared with those measured using the XE-2100 PLT-O method in 48 samples with platelet counts less than 20.0×10 9 /L.…”

“…The recently launched hematology autoanalyzer Sysmex XN-2000 (Sysmex, Kobe, Japan) provides a new method (PLT-F method) for platelet counting based on a fluorescent RNA staining dye named oxazine [1, 2]. The main advantage of the XN-2000's PLT-F method over the former instrument XE-2100's optical method (PLT-O method) is the ability to identify apoptotic white cells and not count them as platelets [2].…”

“…The main advantage of the XN-2000's PLT-F method over the former instrument XE-2100's optical method (PLT-O method) is the ability to identify apoptotic white cells and not count them as platelets [2]. The XN-2000's PLT-F method was reported to estimate platelets in thrombocytopenic samples more accurately than does the PLT-O method [1]. However, the performance of XN-2000's PLT-F method still needs to be evaluated, including samples with broad range of platelet counts.…”

Reticulated platelets: analytical aspects and clinical utility

“…It is now part of the fluorescent platelet assay, which uses a proprietary oxazine-based dye for staining RNA. Still, IPF is derived from the forward scatter (cell size) versus sideward fluorescence (RNA content) scatterplot [56,57], as shown in Figure 2B. The moderate correlation in IPF between XE-2100 and XN is explained, at least partially, by reduced interference in the XN system [56].…”

Performance evaluation of low platelet count and platelet clumps detection on Mindray BC-6800 hematology analyzer

“…To the Editor, Platelet count is an important parameter to evaluate hemostatic status for clinical decision making [1,2] that is routinely offered by automated blood cell counters [3]. Many factors can influence its accuracy, e.g.…”