Performance evaluation of low platelet count and platelet clumps detection on Mindray BC-6800 hematology analyzer

Fuster, Óscar; Andino, Belinda; Laíz, Begoña

doi:10.1515/cclm-2015-0409

Cited by 7 publications

(6 citation statements)

References 6 publications

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“…Nevertheless, none of them have always been effective, so that more approaches need to be developed. The Mindray BC-6800 hematology analyzer's optical fluorescence platelet count is obtained from the reticulocyte channel ( 7 ). Accidentally, we found that the optical fluorescence platelet counts were significantly higher than impedance platelet counts when using BC-6800 Hematology Analyzer to count platelets of EDTA-PTCP samples, and the optical fluorescence platelet counts were comparable with the platelet counts of additionally drawn citrate anticoagulated blood sample (without platelet aggregates).…”

Section: Introductionmentioning

confidence: 99%

Correction of spurious low platelet counts by optical fluorescence platelet counting of BC-6800 hematology analyzer in EDTA-dependent pseudo thrombocytopenia patients

Bao¹,

Wang²,

Wang³

et al. 2020

Transl Cancer Res TCR

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Background To evaluate the dissociation effect of optical fluorescence platelet counting of BC-6800 hematology analyzer on ethylene diamine tetraacetic acid-dependent pseudo thrombocytopenia (EDTA-PTCP) samples. Methods Twenty-three finally identified EDTA-PTCP samples were recruited in this study using criteria as follow: (I) impedance platelet counts lower than 100×10 9 /L with instrument “platelet aggregation” flag; (II) existence of platelet clumps in the blood smear; (III) obviously higher platelet counts without “platelet aggregation” flag and no platelet clumps in blood smear after repeating phlebotomy using citrate anticoagulated tubes. The BC-6800 hematology analyzer and the XE-2100 hematology analyzer were used to test 23 EDTA-PTCP samples and 30 controls on both the impedance channel and the reticulocyte channel. The dissociation rate was defined as optical fluorescence platelet counts in the EDTA tubes/impedance platelet counts in citrate tubes ×100%. Results BC-6800 analyzer’s optical fluorescence platelet counts of EDTA-PTCP samples were significantly higher than impedance platelet counts (t=4.33, P=0.00) and comparable with the platelet counts of re-collected samples in tubes containing citrate anticoagulant. On BC-6800 hematology analyzer, 22 of 23 EDTA-PTCP samples showed a dissociation rate greater than 80%, and the average dissociation rate was 93%. On the XE-2100 hematology analyzer, 1 of the 17 EDTA-PTCP samples showed a dissociation rate greater than 80%, and the average dissociation rate was 56%. Conclusions Optical fluorescence platelet counting of BC-6800 Hematology Analyzer is effective for the correction of spurious low platelet counts in EDTA-PTCP patients, and its dissociation effect on EDTA-PTCP samples was independent of fluorescent dye staining.

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Section: Introductionmentioning

confidence: 99%

Correction of spurious low platelet counts by optical fluorescence platelet counting of BC-6800 hematology analyzer in EDTA-dependent pseudo thrombocytopenia patients

Bao¹,

Wang²,

Wang³

et al. 2020

Transl Cancer Res TCR

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“…Briefly, the XN-9000 and the BC-6000 analyzers perform a 5-part DIFF, RET count, NRBC count, with flags appearing in the presence of abnormal results. 7 , 14 , 15 Both analyzers use a combination of flow cytometry and fluorescence with lysing buffers for leukocyte DIFF and identification of abnormal cells. A separate channel for NRBC assessment is also available in the BC-6800.…”

Section: Introductionmentioning

confidence: 99%

Assessment of blood sample stability for complete blood count using the Sysmex XN-9000 and Mindray BC-6800 analyzers

Buoro

Mecca

Seghezzi

et al. 2016

Revista Brasileira de Hematologia e Hemoterapia

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BackgroundDifferent hematological analyzers have different analytical performances that are often reflected in the criteria for sample stability of the complete blood count. This study aimed to assess the stability of several hematological parameters using the XN-9000 Sysmex and BC-6800 Mindray analyzers.MethodsThe impact of storage at room temperature and 4 °C was evaluated after 2, 4, 6, 8, 24, 36 and 48 h using ten normal and 40 abnormal blood samples. The variation from the baseline measurement was evaluated by the Steel–Dwass–Critchlow–Fligner test and by Bland–Altman plots, using quality specifications and critical difference as the total allowable variation.ResultsRed blood cells and reticulocyte parameters (i.e. hematocrit, mean corpuscular volume, mean corpuscular hemoglobin concentration, red blood cell distribution width, immature reticulocyte fractions, low-fluorescence reticulocytes, middle-fluorescence reticulocytes, high fluorescence mononuclear cells) showed less stability compared to leukocyte and platelet parameters (except for monocyte count and mean platelet volume). The bias for hematocrit, mean corpuscular volume, mean corpuscular hemoglobin concentration and red blood cell distribution width coefficient of variation was higher than the critical difference after 8 h using both analyzers.ConclusionBlood samples measured with both analyzers do not show analytically significant changes in up to 2 h of storage at room temperature and 4 °C. However, the maximum time for analysis can be extended for up to 8 h when the bias is compared to the critical difference.

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“…In a comparative analysis of the usefulness of platelet-clump flagging among the Sysmex, Beckman Coulter, and ADVIA (Siemens Healthcare Diagnostics, Eschborn, Germany) CBC analyzers, the Sysmex analyzer demonstrated the highest sensitivity of 67% [18]. BC-6800, the predecessor of the BC-6800Plus, exhibited a sensitivity of 94% and specificity of 87% for platelet-clump flagging [19]. Platelet clumping occurs very rarely, and its incidence rate varies with population characteristics, resulting in diverse results on flagging efficiency.…”

Section: Discussionmentioning

confidence: 99%

Integration of an MC-80 Digital Image Analyzer With an Automated BC-6800Plus Hematology Analyzer Enables Accurate Platelet Counting in Samples With EDTA-Induced Pseudothrombocytopenia

So,

Huh,

Kim

et al. 2024

Ann Lab Med

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Background: EDTA-induced pseudothrombocytopenia (PTCP) during whole blood collection requires significant laboratory resources to obtain accurate results. We evaluated platelet-deaggregation function in EDTA-induced PTCP and platelet-clump flagging by the BC-6800Plus hematology analyzer using integrated digital image analysis.Methods: We prospectively collected 132 whole blood samples suspected of platelet clumping (102 in EDTA and 30 in sodium citrate) from 88 individuals. We compared platelet counts determined using the platelet count by impedance (PLT-I) function of the DxH 900 hematology analyzer and the PLT-I or optical platelet count (PLT-O) function of the BC-6800Plus. Platelet clumping was verified through manual inspection and the MC-80 digital image analyzer.Results: Among the 132 whole blood samples, 43 EDTA samples showed platelet clumping. The DxH 900 PLT-I and BC-6800Plus PLT-I results demonstrated a strong correlation (r = 0.711) for the EDTA samples but only a moderate correlation with the BC-6800Plus PLT-O results (r = 0.506 and 0.545, respectively). The BC-6800Plus PLT-O results were consistent with the sodium citrate platelet counts, with a median dissociation rate of 102.5% (range, 74.9%-123.1%). The DxH 900 and BC-6800Plus analyzers had sensitivity values of 0.79 and 0.72, respectively, for platelet-clump flagging. When integrating the MC-80 digital image analysis results, the sensitivity of BC-6800Plus improved to 0.89 (standard mode) or 1.0 (PLT-Pro mode).Conclusions: BC-6800Plus PLT-O measurement results are close to the actual values obtained by platelet deaggregation with PTCP samples. Integrating the BC-6800Plus with a digital imaging analyzer effectively improved the diagnosis of PTCP and reduced the requirement for additional laboratory procedures.

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Performance evaluation of low platelet count and platelet clumps detection on Mindray BC-6800 hematology analyzer

Cited by 7 publications

References 6 publications

Correction of spurious low platelet counts by optical fluorescence platelet counting of BC-6800 hematology analyzer in EDTA-dependent pseudo thrombocytopenia patients

Correction of spurious low platelet counts by optical fluorescence platelet counting of BC-6800 hematology analyzer in EDTA-dependent pseudo thrombocytopenia patients

Assessment of blood sample stability for complete blood count using the Sysmex XN-9000 and Mindray BC-6800 analyzers

Integration of an MC-80 Digital Image Analyzer With an Automated BC-6800Plus Hematology Analyzer Enables Accurate Platelet Counting in Samples With EDTA-Induced Pseudothrombocytopenia

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