New Estimation Methods of Bacterial Concentration by Measuring ATP Changes during Incubation.

Fujikawa, Hiroshi; Morozumi, Satoshi

doi:10.3358/shokueishi.43.155

Cited by 6 publications

(23 citation statements)

References 15 publications

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“…They were cultured and washed with phosphate bu#er, pH 7.0, with 0.005ῐ (vol/vol) Tween 80 in the same manner as done by us previously 12) . Cells were finally suspended in 5 mL of saline (0.85ῐ wt/vol NaCl solution) at the final concentration of 10 9 CFU/mL.…”

Section: Bacterial Cell Preparationmentioning

confidence: 99%

“…Bacterial strains of Escherichia coli 1952 and Staphylococcus aureus 2246 were those studied in our previous study 12) . They were cultured and washed with phosphate bu#er, pH 7.0, with 0.005ῐ (vol/vol) Tween 80 in the same manner as done by us previously 12) .…”

Section: Bacterial Cell Preparationmentioning

confidence: 99%

“…Sample suspensions in glass tubes were cultured with shaking at 120 rpm at 35῏ 12) . At 1-hour intervals during incubation, portions (about 3 mL) of the suspensions were taken and cooled in ice water.…”

Section: Incubationmentioning

confidence: 99%

“…Thus, the methods can predict the concentration of living bacteria only, because dead cells cannot proliferate in culture media. The methods were successfully applied for estimation of bacterial cells cultured in a medium 12) . Next, the methods should be evaluated with commercial foods, to assess their potential as rapid methods in the food industry.…”

Section: Introductionmentioning

confidence: 99%

“…Under these circumstances, we developed new, kinetic ATP measurement methods for prediction of bacterial concentrations in food samples with a conventional firefly luminometer 12) . The methods are an end point method and a delay method.…”

Section: Introductionmentioning

confidence: 99%

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Estimation of Bacterial Concentrations in Commercial Foods by Measuring ATP Changes during Incubation.

Fujikawa¹,

Morozumi²

2003

J. Food Hyg. Soc. Jpn

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Recently we have developed new kinetic methods of bacterial cell estimation in food products by measurement of the increase in bacterial adenosine-triphosphate (ATP) content during incubation using a conventional firefly luminometer [Fujikawa and Morozumi: Jpn. J. Food Hyg. (2002)]. The methods were the end point method and the delay method. We, in this study, evaluated these methods for bacterial concentration estimation in commercial foods. The methods were successfully applied to food products inoculated with Escherichia coli or Staphylococcus aureus. The methods were then used to estimate bacterial concentrations in commercial foods. For estimation, the kinetic parameter values were determined first from the data of bacterial strains studied previously. The bacterial concentrations estimated with these parameter values were slightly smaller than the measured concentrations for most of the food samples. The parameter values obtained with the least-squares method gave better estimations in both methods. The delay method predicted the bacterial concentrations in food products better than the end point method. This study showed that with appropriate parameter values, these methods could be useful tools for bacterial concentration estimation of commercial foods.

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Section: Bacterial Cell Preparationmentioning

confidence: 99%

Section: Bacterial Cell Preparationmentioning

confidence: 99%

Section: Incubationmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Estimation of Bacterial Concentrations in Commercial Foods by Measuring ATP Changes during Incubation.

Fujikawa¹,

Morozumi²

2003

J. Food Hyg. Soc. Jpn

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Variation in detection limits between bacterial growth phases and precision of an ATP bioluminescence system

Vogel

Tank

Goodyear

2013

Lett Appl Microbiol

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Significance and Impact of the Study Surface hygiene is a critical component of food safety and infection control; increasingly, ATP detection by bioluminescence is used to evaluate surface hygiene and effective cleaning. This is the first study to show that the number of living and potentially infectious bacteria remaining when the device reads zero varies between the different bacterial life cycle phases: lag, log, stationary and death. ATP device users need to be aware of this information to use the devices appropriately. AbstractTo determine the detection limits of the SystemSure Plus, Escherichia coli and Staphylococcus aureus growth curve samples were taken in lag (1 h), log (6 h), stationary (12 h) and death phases (E. coli 144 h, Staph. aureus 72 h). At each time point, the log 10 CFU ml À1 was determined for the dilution where the SystemSure read 0 relative light units (RLU). Average detection limits were E. coli: lag 6Á27, log 5Á88, stationary 7Á45 and death 6Á88; Staph. aureus: lag 4Á37, log 5Á15, stationary 7Á88 and death 7Á57. Between-run precision was determined with positive control; within-run precision with positive control, lag and log growth for each bacteria. Within-run precision mean RLU (CV): positive control 274 (12%), E. coli lag 1 (63%), log 2173 RLU (19%), Staph. aureus lag 2 (58%) and log 5535 (18%). Between-run precision was 232 (16%). The precision is adequate with most values within the 95% confidence interval. The detection limit varied by 3Á51 log 10 for Staph. aureus and 1Á47 log 10 for E. coli. The lowest detection limits were during E. coli log and Staph. aureus lag phases; the highest was during stationary phase. These results suggest that organism identification and growth phase both impact ATP RLU readings.

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Rapid and Simple Detection of Coliform Bacteria in Milk Using Menadione-Catalyzed Luminol Chemiluminescence Assay

Kawasaki¹,

Yamashoji²,

Asakawa³

et al. 2007

J. Food Sci. Technol. Res.

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Menadione-catalyzed luminol chemiluminescence assay was evaluated for rapid detection of coliform bacteria in milk. The assay was evaluated using raw milk (non-pasteurized) samples and pasteurized milk samples spiked with Escherichia coli, Enterobacter aerogenes, Klebsiella oxytoca, Citrobacter freundii. The detection limit in this assay was +* / ῌ+*. CFU/ml using a 30-well plate with BGLB broth. Our study revealed that coliform contamination of +* + ῌ+* * CFU/ml of milk could be detected after +2 h of incubation at-/ in BGLB medium. Moreover, this assay detected gas production in samples of BGLB after only +2 h of a .2-h incubation. As this assay is convenient, quick and inexpensive compared to the conventional method, it should be useful for the rapid detection of coliform contamination in pasteurized milk.

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New Estimation Methods of Bacterial Concentration by Measuring ATP Changes during Incubation.

Cited by 6 publications

References 15 publications

Estimation of Bacterial Concentrations in Commercial Foods by Measuring ATP Changes during Incubation.

Estimation of Bacterial Concentrations in Commercial Foods by Measuring ATP Changes during Incubation.

Variation in detection limits between bacterial growth phases and precision of an ATP bioluminescence system

Rapid and Simple Detection of Coliform Bacteria in Milk Using Menadione-Catalyzed Luminol Chemiluminescence Assay

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