Menadione-catalyzed luminol chemiluminescence assay was evaluated for rapid detection of coliform bacteria in milk. The assay was evaluated using raw milk (non-pasteurized) samples and pasteurized milk samples spiked with Escherichia coli, Enterobacter aerogenes, Klebsiella oxytoca, Citrobacter freundii. The detection limit in this assay was +* / ῌ+*. CFU/ml using a 30-well plate with BGLB broth. Our study revealed that coliform contamination of +* + ῌ+* * CFU/ml of milk could be detected after +2 h of incubation at-/ in BGLB medium. Moreover, this assay detected gas production in samples of BGLB after only +2 h of a .2-h incubation. As this assay is convenient, quick and inexpensive compared to the conventional method, it should be useful for the rapid detection of coliform contamination in pasteurized milk.
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