New cytotoxic steroids from the leaves of Clerodendrum trichotomum

Bioactive Diterpenoids from Clerodendrum kiangsiense

“…Phytochemical investigations on Clerodendrum species revealed various diterpenoids in the plants, which showed antibacterial and cytotoxic activities [8,9]. However, the phytochemical composition of the stems and roots of C. kiangsiense have not been full characterized.…”

“…Preparations of the leaves, branches and stems of Clerodendrum have been used in folk medicine to treat different diseases such as cancer, catarrhal affections of the lungs, fever, inflammation, skin diseases and asthma [6,7]. Phytochemical investigations on Clerodendrum species revealed various diterpenoids in the plants, which showed antibacterial and cytotoxic activities [8,9]. However, the phytochemical composition of the stems and roots of C. kiangsiense have not been full characterized.…”

Traditional uses and pharmacological properties of Clerodendrum phytochemicals

“… Decreased MDA level, increase CAT activity and GSH level 113 Methanolic extracts from C. siphonanthus In vitro Thiocyanate method, DPPH-radicals 0–120 mg/ml Scavenging lipid peroxide (IC 50 = 8 mg/ml) and DPPH radicals (IC 50 = 7 mg/ml) 114 Anti-cancer activity Methanolic extract from C. serratum In vivo DMBA-induced skin tumorigenesis in male mice 300, 600 and 900 mg/kg, i.g. Curtailed tumor development 115 , 116 Methanolic extract from C. serratum In vivo DLA cell model 100 and 200 mg/kg Reduced skin papilloma incidence and multiplicity 117 Cryptojaponol, fortunin E, 12-methoxy-6,11,14,16-tetrahydroxy-17(15→16)-abeo-5,8,11,13-abietatetraen-3,7-dione In vitro HL-60, SMMC-7721, lA-549, MCF-7 cell lines 1.8–5.0 μM Exhibited cytotoxicity 89 Compounds 45, 70, 76, 78, 81, and 82 In vitro BGC-823, Huh-7, KB, KE-97, and Jurkat 0.83–50.99 μM Exhibited cytotoxicity 41 Total flavonoids from C. Bungei In vitro HepG 2 0.025–250 μg/ml Inhibited HepG 2 cells proliferation 119 Trichotomone In vitro A549, Jurkat, BGC-823 and 293T WT 7.51–19.38 μM Exhibited cytotoxicity 43 Compounds 240 and 243 In vitro Hela cell 28.92–35.67 μg/ml Exhibited moderate cytotoxicity 82 Anti-bacterial activity Methanolic extract from C. siphonanthus In vitro Klebsiella pneumoniae , Proteus mirabilis , Salmonella typhi , ...…”

Anticancer Activity Of Plant Genus Clerodendrum (Lamiaceae): A Review

“…Lima senyawa yang berhasil diidentifikasi dalam tanaman genus ini yaitu apigenin 7-glucoside, hispidulin, scutellarein-7-O-beta-D-glucuronate, acteoside dan verbascoside menunjukan interaksi dengan protein kanker melalui simulasi penambatan molekul dan berpotensi menimbulkan efek terapi (Gogoi et al 2017 Wang, Zhu, et al 2013;Wang, Xiong, et al 2013;Xu et al 2013 memiliki aktivitas antikanker dari tanaman genus Clerodendrum adalah pheophorbide-related compounds (purpurin 7 dimethyl ester, pheophorbide a, methyl (10S)-hydroxypheophorbide a) (Cheng et al 2001); asam lemak (asam oleat, asam oktadekanoat, asam n-heksadekanoat, dan 6-metil-2-heptanon) (Erukainure et al 2016); asam oleanolik dan clerodinin A (Sannigrahi et al 2012); abitane diterpenoid (trichotomone, trichotomone D, trichotomone F, uncinatone, mandarone E, teuvincenone E, dan trichotomone H) (Wang, Xiong, et al 2013;Wang, Zhu, et al 2013) dan dua senyawa steroid (Xu et al 2013). Mekanisme antikanker beberapa tanaman genus Clerodendrum melalui aktivitas fotositoksisitas (Nakamura et al 1996;Choi et al 2014;Cheng et al 2001), apoptosis, antiproliferatif dan anti-migrasi (Sun et al 2013;Chacko et al 2015).…”

“…Dengan menggunakan CellTiter Glo TM luminescent cell viability assay, nilai IC50 tujuh senyawa abitane diterpenoid pada sel kanker tersebut antara 0,83 -50,99 μM (Wang, Zhu, et al 2013;Wang, Xiong, et al 2013). Dua senyawa steroid yang diisolasi daun C. trichotomum menunjukan aktivitas sitotoksik pada sel HeLa dengan nilai IC50 35,67 dan 28,92 μg/ml menggunakan metode MTT (Xu et al 2013) …”