New cyclohexylethanoids from the leaves of Clerodendrum trichotomum

Xu, Ruilan; Wang, Rui; Ha, Wei; Shi, Yan‐Ping

doi:10.1016/j.phytol.2013.10.010

Cited by 14 publications

(8 citation statements)

References 13 publications

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“…Clerodendrum, a genus of flowering plants in the family Lamiaceae (Verbenaceae) , is 29 widely distributed across China, Korea, Taiwan, Japan and India (Yamazaki., 1993). Clerodendrum 30 trichotomum has been used for medicinal substances to act against human tumor cells (Wang et al, 2013a) 31 and many new medicinal substances have been found in its tissues (Wang et al, 2013b;Xu et al, 2014). 32 Moreover, C. trichotomum shows strong abiotic stress resistance and is easy to cultivate and manage 33 (Mahmuc et al, 2008).…”

Section: Introduction 28mentioning

confidence: 99%

Selection of appropriate reference genes for quantitative real-time PCR in Clerodendrum trichotomum

Yang

Hua

Yue

et al. 2019

Preprint

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Abstrsct 12 Clerodendrum trichotomum, an important medicinal plant, has excellent salt tolerance and beautiful 13 ornamental character. However, reliable reference genes for quantitative real-time PCR data (qRT-PCR) in 14 C. trichotomum have not been investigated. Using our previous transcriptome data, 17 reference genes were 15 selected in different tissues (leaves, flowers, fruits, stems, and roots) and under various abiotic stresses (salt, 16 drought, flood, and heat) for C. trichotomum, using four different reference gene analysis software types: 17 GeNorm, NormFinder, BestKeeper and ReFinder. The results identified RPL, ACT and HSP70 as the three 18 most suitable reference genes for tissues. Genes ACT and AP-2 were most stably expressed under drought 19 stress; MDH and UBCE2 were stable under flooding stress; RPL and UBCE2 were most stable under salt 20 stress; and MDH and EF-1A were most appropriate under heat stress. For abiotic treatments, RPL, MDH 2 21and AP-2 were the most stable reference genes; and AP-2, RPL and ACT were stably expressed in all 22 examined samples. The expression profile of the genes for Na + /H + Exchanger1 (ClNHX1) and laccase 23 (ClLAC) were selected to validate the stability of the determined reference genes. Our study provided 24 reliable normalization for gene expression analysis and ensured more accurate data for further molecular 25 mechanism research in C. trichotomum. 26

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Section: Introduction 28mentioning

confidence: 99%

Selection of appropriate reference genes for quantitative real-time PCR in Clerodendrum trichotomum

Yang

Hua

Yue

et al. 2019

Preprint

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“…Clerodendrum trichotomum , known as ‘Chou-Wu-Tong’ in China, is an ornamental plant found in wild areas with a temperate climate in China, Japan, Korea, and the Philippines [1]. As a widespread broad-leaved understory shrub, C .…”

Section: Introductionmentioning

confidence: 99%

SSR marker development in Clerodendrum trichotomum using transcriptome sequencing

et al. 2019

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Clerodendrum trichotomum, a member of the Lamiaceae (Verbenaceae) family, is an ornamental plant widely distributed in South Asia. Previous studies have focused primarily on its growth characteristics, stress resistance, and pharmacological applications; however, molecular investigations remain limited. Considering germplasm conservation and the extensive applications of this plant, it is necessary to explore transcriptome resources and SSR makers for C. trichotomum. In the present study, RNA sequencing was used to determine the transcriptome of C. trichotomum. Subsequently, unigene annotations and classifications were obtained, and SSRs were mined with MIcroSAtellite. Finally, primer pairs designed with Oligo 6.0 were selected for polymorphism validation. In total, 127,325,666 high-quality reads were obtained, and 58,345 non-redundant unigenes were generated, of which 36,900 (63.24%) were annotated. Among the annotated unigenes, 35,980 (97.51%) had significant similarity to 607 species in Nr databases. In addition, a total of 6,444 SSRs were identified in 5,530 unigenes, and 200 random primer pairs were designed for polymorphism validation. Furthermore, after primary polymorphism identification, 30 polymorphic primer pairs were selected for the further polymorphism screening, and 200 alleles were identified, 197 of which showed polymorphism. In this work, a large number of unigenes were generated, and numerous SSRs were detected. These findings should be beneficial for further investigations into germplasm conservation and various applications of C. trichotomum. These results should also provide a solid foundation for future molecular biology studies in C. trichotomum.

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“…A variety of constituents have been isolated and characterized from this genus, including: monoterpene and its derivatives, 10 sesquiterpene, 11 diterpenoids, 12 , 13 triterpenoids, 14 , 15 flavonoid and flavonoid glycosides, 16 phenylethanoid glycosides, 17 , 18 steroids and steroid glycosides, 19 cyclohexylethanoids, 20 anthraquinones, 21 cyanogenic glycosides, 22 and others. Some of these constituents have been evaluated with a number of biological properties, mainly including anti-inflammatory and anti-nociceptive, anti-oxidant, anti-hypertensive, anticancer, antimicrobial, anti-diarrheal, hepatoprotective, hypoglycemic and hypolipidemic, memory enhancing and neuroprotective, and other activities.…”

Section: Introductionmentioning

confidence: 99%

Traditional uses and pharmacological properties of Clerodendrum phytochemicals

Wang

Luan²,

et al. 2018

Journal of Traditional and Complementary Medicine

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Clerodendrum is a genus of ca. 500 species in the family Lamiaceae and widely distributed throughout the whole world. Up to now, many species of this genus have been described in various indigenous systems of medicine and are used in preparation of folklore medicines for the treatment of various life-threatening diseases, and more than eleven species of the Clerodendrum genus have been very well studied for their chemical constituents and biological activities, and 283 compounds, including monoterpene and its derivatives, sesquiterpene, diterpenoids, triterpenoids, flavonoid and flavonoid glycosides, phenylethanoid glycosides, steroids and steroid glycosides, cyclohexylethanoids, anthraquinones, cyanogenic glycosides, and others have been isolated and identified. Pharmacological studies have shown that these compounds and extracts from the Clerodendrum genus have extensive activities, such as anti-inflammatory and anti-nociceptive, anti-oxidant, anti-hypertensive, anticancer, antimicrobial, anti-diarrheal, hepatoprotective, hypoglycemic and hypolipidemic, memory enhancing and neuroprotective, and other activities. In this review, we attempt to highlight over phytochemical progress and list the phytoconstituents isolated from the genus Clerodendrum reported so far. The biological activities of this genus are also covered.

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New cyclohexylethanoids from the leaves of Clerodendrum trichotomum

Cited by 14 publications

References 13 publications

Selection of appropriate reference genes for quantitative real-time PCR in Clerodendrum trichotomum

Selection of appropriate reference genes for quantitative real-time PCR in Clerodendrum trichotomum

SSR marker development in Clerodendrum trichotomum using transcriptome sequencing

Traditional uses and pharmacological properties of Clerodendrum phytochemicals

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