New concepts for quantitative bioprocess research and development

Sonnleitner, Bernhard

doi:10.1007/bfb0102335

Cited by 14 publications

(7 citation statements)

References 67 publications

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“…Cultivations were performed in a high performance compact loop reactor (research bioreactor, in-house construct, described in Sonnleitner, 1996) with a typical working volume between 2.5 and 3.0 L. The bioreactor was equipped with sensors for pH, pO 2 , T, p, turbidity (AF46, Aquasant AG, CH) and two in situ fluorescencesensors (Aquasant AG, CH, described in Broger et al, 2009) for the on-line determination of the eGFP fluorescence as well as a mass flow meter and an exhaust gas analyser (Siemens AG, CH) to monitor CO 2 and O 2 . The following cultivation conditions were kept constant in the bioreactor: temperature at 30.0 ± 0.1 • C; pH at 5.0 ± 0.05 (by titration with 25% NH 3 solution); stirrer speed at 2000 rpm; foam breaker speed at 1500 rpm; gas flow rate at 2 NL min −1 .…”

Section: Bioprocessmentioning

confidence: 99%

Real-time on-line flow cytometry for bioprocess monitoring

Broger

Odermatt

Huber

et al. 2011

Journal of Biotechnology

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Section: Bioprocessmentioning

confidence: 99%

Real-time on-line flow cytometry for bioprocess monitoring

Broger

Odermatt

Huber

et al. 2011

Journal of Biotechnology

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“…Especially biosensors are extremely interesting for a sensitive and selective monitoring of single components even in complex bioprocess media. Excellent reviews are available which describe the function in general and different samples of application of these sensor classes [1,6,[11][12][13][14][15][16].…”

Section: Bioprocess Monitoringmentioning

confidence: 99%

“…Several applications for use in bioprocess monitoring are described and reviewed in the literature [1][2][3][4][5][6]. However, additionally new analytical systems or procedures must be developed to meet the special demands of modern and future bioprocessing or additional efforts are necessary to adapt the already existing systems for a efficient bioprocess monitoring.…”

Section: Introductionmentioning

confidence: 99%

Bioanalytics: detailed insight into bioprocesses

Scheper

Hitzmann

Stärk

et al. 1999

Analytica Chimica Acta

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The principles of bioanalytical systems for an on-line bioprocess monitoring are described within this paper. These sensor systems can be interfaced to the bioprocess in different ways according to the needs of the single bioprocess. Modular systems are necessary, which can fit exactly to the needs of the single process. Invasive as well as non-invasive bioanalytical tools are described and discussed in detail. Immunosensors give the possibility to monitor high molecular weight components within short time intervals. Non-invasive optical sensors allow the direct monitoring of various analytes such as oxygen pH for the complex fluorescence behavior of the bioprocess medium. These so-called fluorescence sensors offer the possibility to monitor intra-as well as extracellular components without interfering with the bioprocess. An industrial example for the application of bioanalytical tools for a process optimization are presented in this application. Here a biosensor system is used to optimize the downstreaming of molasses on a technical scale. The economic as well ecological advantages are discussed.

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“…However, when the oxygen transfer capacity of a reactor under constant operating conditions is roughly known [23], one can estimate the fraction of O 2 in the exhaust gas at limiting oxygen transfer. In the experimental setup used, this value was expected around 10% (see also Fig.…”

Section: Proportional-integral (Pi) Control Of O 2 In Exhaust Gasmentioning

confidence: 99%

Robust oxygen supply by controlled addition of hydrogen peroxide to microbial cultures

Sonnleitner

Hahnemann

1997

Bioprocess Engineering

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The supply of oxygen can be improved by the direct addition of hydrogen peroxide to cultures of aerobic microbes expressing suf®cient amounts of catalase. This is of special interest if normal aeration has to be kept low, for instance, in order to minimize evaporation of volatile compounds (either substrates or products) or to minimize foaming. Also, if the mechanical power input to the bioreactor is or has to be limited, addition of hydrogen peroxide may be useful.The appropriate dosage of hydrogen peroxide can be simply determined by a controller of the oxygen partial pressure or of the oxygen content in the exhaust gas using various control algorithms. The added hydrogen peroxide can be either a stabilized concentrate, e.g. 30%, or any dilute form of this. In high density cultures, Pseudomonas cells tolerated even harsh controller disturbances. This approach proved to be very robust and reliable.

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New concepts for quantitative bioprocess research and development

Cited by 14 publications

References 67 publications

Real-time on-line flow cytometry for bioprocess monitoring

Real-time on-line flow cytometry for bioprocess monitoring

Bioanalytics: detailed insight into bioprocesses

Robust oxygen supply by controlled addition of hydrogen peroxide to microbial cultures

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