Bioanalytics: detailed insight into bioprocesses

Abstract

The principles of bioanalytical systems for an on-line bioprocess monitoring are described within this paper. These sensor systems can be interfaced to the bioprocess in different ways according to the needs of the single bioprocess. Modular systems are necessary, which can fit exactly to the needs of the single process. Invasive as well as non-invasive bioanalytical tools are described and discussed in detail. Immunosensors give the possibility to monitor high molecular weight components within short time intervals. Non-invasive optical sensors allow the direct monitoring of various analytes such as oxygen pH for the complex fluorescence behavior of the bioprocess medium. These so-called fluorescence sensors offer the possibility to monitor intra-as well as extracellular components without interfering with the bioprocess. An industrial example for the application of bioanalytical tools for a process optimization are presented in this application. Here a biosensor system is used to optimize the downstreaming of molasses on a technical scale. The economic as well ecological advantages are discussed.

Offline glucose biomonitoring in yeast culture by polyamidoamine/ cysteamine‐modified gold electrodes

“…Furthermore, this may cause production of undesirable byproducts, and these factors affect fermentation yield and product quality directly. Hence, optimizing product quality 6 and minimizing process cost 7 are the main goals in beverage and food industries. Consequently, according to the importance of glucose in the bioprocesses, effective, reliable, fast, and real-time monitoring systems should be required for controlling the system.…”

Evaluation of wavelength selection methods for 2D fluorescence spectra applied to bioprocesses characterization

“…This non-invasive technique can be considered an evolution of the fluorescence sensor that could monitor only the fluorophore NAD(P)H and then would obtain information on cellular metabolism. Once the 2D fluorescence spectroscopy is capable of monitoring all fluorophores, it can yield information not only about cellular metabolism, but also about the cultivation medium (Scheper et al 1999, Ganzlin et al 2007.…”

“…Due to the high dependence of biological systems (microorganisms and produced metabolites) on the physical and chemical characteristics of the cultivation medium, bioprocesses can become quite complex (Scheper et al, 1996, Scheper et al, 1999. Therefore, the maintenance of product quality and reduction in production costs of these processes are highly associated with monitoring and controlling the operating conditions.…”

Two-dimensional fluorescence spectroscopy: A novel approach for controlling fed-batch cultivations

“…The in situ fluorescence measurements are carried out using the BioView ® sensor (Delta Light and Optics, Denmark), a robust instrument designed for industrial applications (Marose et al, 1998;Scheper et al, 1999). The system is based on multichannel fluorescence detection.…”