Two-dimensional fluorescence spectroscopy: A novel approach for controlling fed-batch cultivations

Hantelmann, K.; Kollecker, M.; Hüll, D.; Hitzmann, Bernd; Scheper, Thomas

doi:10.1016/j.jbiotec.2005.07.016

Cited by 74 publications

(60 citation statements)

References 19 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…This will have interesting biotechnological implications for the fermentation industries that use this microorganism; for example, the results open the possibility of designing a monitoring system (26,65) based on the analysis of hyphal differentiation. Besides this, the developmental features described will contribute to a better understanding and/or interpretation of previously reported data on submerged cultures.…”

Section: Discussionmentioning

confidence: 99%

Mycelium Differentiation and Antibiotic Production in Submerged Cultures ofStreptomyces coelicolor

Manteca

Álvarez

Salazar

et al. 2008

Appl Environ Microbiol

136

157

View full text Add to dashboard Cite

Despite the fact that most industrial processes for secondary metabolite production are performed with submerged cultures, a reliable developmental model for Streptomyces under these culture conditions is lacking. With the exception of a few species which sporulate under these conditions, it is assumed that no morphological differentiation processes take place. In this work, we describe new developmental features of Streptomyces coelicolor A3(2) grown in liquid cultures and integrate them into a developmental model analogous to the one previously described for surface cultures. Spores germinate as a compartmentalized mycelium (first mycelium). These young compartmentalized hyphae start to form pellets which grow in a radial pattern. Death processes take place in the center of the pellets, followed by growth arrest. A new multinucleated mycelium with sporadic septa (second mycelium) develops inside the pellets and along the periphery, giving rise to a second growth phase. Undecylprodigiosin and actinorhodin antibiotics are produced by this second mycelium but not by the first one. Cell density dictates how the culture will behave in terms of differentiation processes and antibiotic production. When diluted inocula are used, the growth arrest phase, emergence of a second mycelium, and antibiotic production are delayed. Moreover, pellets are less abundant and have larger diameters than in dense cultures. This work is the first to report on the relationship between differentiation processes and secondary metabolite production in submerged Streptomyces cultures.Streptomyces is a soil bacterium that produces numerous clinically useful antibiotics (1, 54, 66), as well as many molecules that affect eukaryotic systems, such as inducers of eukaryotic cellular differentiation, inducers and inhibitors of apoptosis (59), and protein C kinase inhibitors with antitumor activity (such as staurosporine and others) (48, 57). Moreover, its remarkably complex developmental cycle makes this microorganism an interesting subject for study. The traditional developmental cycle of this bacterium describes two differentiated mycelial structures, a substrate (vegetative) mycelium and an aerial (reproductive) mycelium (10,25,33). In the substrate mycelium, septa are thought to be widely spaced and to define compartments containing several nucleoids (10, 63). After a short growth arrest phase characterized by reduced macromolecular synthesis (25), aerial hyphae develop from simple branching from substrate mycelium (29). Finally, the tips of the aerial mycelium differentiate into hydrophobic spore chains (8). Recently, we have been able to extend what is known about the developmental cycle in surface confluent cultures a great deal. Our main contribution has been to reveal the existence of a very young compartmentalized mycelium that dies following an orderly pattern, leaving alternating live and dead segments in the same hypha (37). Subsequently, the remaining live mycelium grows in successive waves that vary according to the density of the ...

show abstract

Section: Discussionmentioning

confidence: 99%

Mycelium Differentiation and Antibiotic Production in Submerged Cultures ofStreptomyces coelicolor

Manteca

Álvarez

Salazar

et al. 2008

Appl Environ Microbiol

136

157

View full text Add to dashboard Cite

show abstract

“…The initial and final steps in the implementation of EEM/SFS-based analytical methods generally requires the use of chemometrics, first to assess the reproducibility and robustness of the measurement itself, and second to produce an output in terms of a qualitative result or quantitative value. The second aspect is outside the scope of this paper, but there are many reviews available describing the various applications in biotechnology, food science [71], and environmental analysis [72][73][74][75][76][77], for example. The application of chemometrics to the fluorescence data analysis used for calibration/standardization can generally be split into four distinct elements or steps: 1.…”

Section: Chemometric Data Analysis For Calibration and Validationmentioning

confidence: 99%

Calibration, standardization, and quantitative analysis of multidimensional fluorescence (MDF) measurements on complex mixtures (IUPAC Technical Report)

Ryder

Stedmon

Harrit

et al. 2017

Pure and Applied Chemistry

View full text Add to dashboard Cite

This IUPAC Technical Report describes and compares the currently applied methods for the calibration and standardization of multi-dimensional fluorescence (MDF) spectroscopy data as well as recommendations on the correct use of chemometric methods for MDF data analysis. The paper starts with a brief description of the measurement principles for the most important MDF techniques and a short introduction to the most important applications. Recommendations are provided for instrument calibration, sample preparation and handling, and data collection, as well as the proper use of chemometric data analysis methods.

show abstract

“…spectroscopy have been highlighted in many recent studies (Boehl et al, 2003;Hantelmann et al, 2006;Ganzlin et al, 2007;Rhee and Kang, 2007;Ödman et al, 2009;Jain et al, 2011;Bogomolov et al, 2011;Rossi et al, 2012). In this technique, the fluorescence emission of metabolites present in the cultivation medium is monitored when they are excited with UV-Visible radiation in a specific wavelength range.…”

Section: Brazilian Journal Of Chemical Engineeringmentioning

confidence: 99%

Evaluation of wavelength selection methods for 2D fluorescence spectra applied to bioprocesses characterization

Masiero

Trierweiler

Farenzena

et al. 2013

Braz. J. Chem. Eng.

View full text Add to dashboard Cite

-In biotechnological processes, the productivity and costs depend strongly on the control of the operating conditions. For this reason, sensors that allow the monitoring of variables of interest become quite important. 2D fluorescence spectroscopy is one promising option among those that are being applied for this purpose. In the present work, three methods were evaluated to select the best excitation/emission wavelength pairs of 2D fluorescence spectra to infer product, substrate and cellular concentrations throughout a fermentation using a multiple linear chemometric model: Exhaustive Search (ES), Stepwise Regression and Genetic Algorithm (GA). The Stepwise Regression presented unsatisfying results, while GA always led to good R 2 values in short computational times. However, for the proposed problem, the ES showed the best performance, finding the global optimum in a few minutes.

show abstract

Two-dimensional fluorescence spectroscopy: A novel approach for controlling fed-batch cultivations

Cited by 74 publications

References 19 publications

Mycelium Differentiation and Antibiotic Production in Submerged Cultures ofStreptomyces coelicolor

Mycelium Differentiation and Antibiotic Production in Submerged Cultures ofStreptomyces coelicolor

Calibration, standardization, and quantitative analysis of multidimensional fluorescence (MDF) measurements on complex mixtures (IUPAC Technical Report)

Evaluation of wavelength selection methods for 2D fluorescence spectra applied to bioprocesses characterization

Contact Info

Product

Resources

About