New components of the chitinolytic system of Trichoderma harzianum

Haran, Shoshan; Schickler, Hedva; Oppenheim, Amos B.; Chet, I.

doi:10.1016/s0953-7562(09)80642-4

Cited by 110 publications

(81 citation statements)

References 17 publications

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“…The protein size revealed by SDS-PAGE for T. harzianum BIO10671 was 42 kDA which was larger than the purified chitinase collected by Deane et al [6] from T. harzianum T198, but similar to the range of endochitinases reported previously by Ulhoa and Peberdy [21], Haran et al [8] and Matsumiya et al [14]. The molecular weight of purified chitinase may differ between species and also within species [19] and it is not known whether they are differently processed gene products from the same gene or from separate genes.…”

Section: Resultssupporting

confidence: 63%

THE EVIDENCE OF NON N-GLYCAN LINKED MANNOSE IN EXOCHITINASE (42 kDa) FROM Trichoderma harzianum BIO10671 GLYCOSYLATION

Mustafa

Zaman

Ramli

et al. 2017

AJSTD

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Chitinase 42 kDa produced by Trichoderma harzianum has been proven as a prime compound to be excreted onto the hyphae of the pathogen causing localised cell wall lysis at the point of interaction. This finally initiate the process of the host cell becomes empty of cytoplasm, disintegrates and shows a rapid collapse. This study investigates the existence of N-glycan linked mannose in chitinase 42 kDa produced by the Malaysian T. harzianum strain BIO10671. The chitinase 42 kDa from T. harzianum BIO10671 was initially purified using anion exchange chromatography prior to a series of experiments such as immunoblotting against the chitinase 42 kDa antibody, lectin staining for detecting any terminal linked mannose, andgalactofuranose detection to determine the presence of galatofuranose components in glycoproteins. The enzyme purification harvested about 12-fold of chitinase 42 kDa from T. harzianum BIO10671 with strong indication of the presence chitinase 42 kDa presence on SDS-Page. This was confirmed by immunoblotting with a strong response around 42 kDa after overnight incubation in chitinase 42 kDa antibody suggesting that the gene for chitinase 42 kDa was greatly expressed in this strain. There are no intervation of galatofuranose on any of the terminal mannose in chitinase 42 kDa as shown by negative results on samples treated with or without endoglycosidase-H and lectin staining. Therefore, it can be concludeed that glycosylation occurred in the chitinase 42 kDa from T. harzianum 42 kDa was not in the form of N-glycan linked mannose as expected.

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Section: Resultssupporting

confidence: 63%

THE EVIDENCE OF NON N-GLYCAN LINKED MANNOSE IN EXOCHITINASE (42 kDa) FROM Trichoderma harzianum BIO10671 GLYCOSYLATION

Mustafa

Zaman

Ramli

et al. 2017

AJSTD

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“…Haran et al (1995) reported an N-acetylglucosaminidase of 102 kDa (CHIT 102) which was expressed by T. haqianwm strain TM when grown on chitin as the sole carbon source. They assumed it to be essentially the same enzyme described by Ulhoa & Peberdy (1991) and the different estimates of its molecular mass were suggested to be the result of the different procedures used.…”

Section: Overviewmentioning

confidence: 99%

Molecular mechanisms of lytic enzymes involved in the biocontrol activity of Trichoderma harzianum

1996

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“…Mycoparasitism by T. harzianum is a complex process, involving recognition of the host, attachment to the mycelium, coiling around the hyphae, partial degradation of the cell wall and penetration of the host mycelium (Elad et al, 1983a, b;Benhamou and Chet, 1993). Cell wall degradation is achieved by six chitin-induced chitinolytic enzymes (comprising two B-1, 4-N-acetylglucoseaminidases and four endochitinases), all of which are required for effective parasitism (Haran et al, 1995). Once, the host mycelium has been penetrated, additional extracellular enzymes such as lipases and proteases are produced to induce degradation of the cell contents .…”

Section: Discussionmentioning

confidence: 99%

“…Antagonism by T. harzianum depends on mycoparasitism rather than antibiosis, as evident from the relatively slight inhibition of mycelial growth of R. solani, particularly AG-3; in dual culture (Shternshis, 2002). Besides, being an aggressive mycoparasite (Elad et al, 1983b;Benhamou and Chet, 1993;Haran et al, 1995;Elad et al, 1982;Chet and Baker, 1981), T. harzianum is also known to enhance plant growth in the absence of any pathogens, probably by producing plant growth promoting metabolites in the rhizosphere (Chang et al, 1986;Kleifeld and Chet, 1992).…”

Section: Discussionmentioning

confidence: 99%

Management of Black Scurf of Potato with Effective Microbes (EM), Biological Potassium Fertilizer (BPF) and Trichoderma harzianum

Rauf¹,

Naz²,

Ahmad³

et al. 2015

IJAB

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The efficacy of soil application with microbial preparations viz. Trichoderma harzianum, effective microbe (EM) culture and biological potassium fertilizer (BPF) was evaluated for the management of soil-borne inoculum of Rhizoctonia solani the cause of black scurf of potato cv. Desiree. Soil application with three dosages of culture suspension of T. harzianum, effective microbe (EM) culture and biological potassium fertilizer (BPF) were applied in the soil to know the efficacy of these treatments in reducing the disease. Soil application with T. harzianum at the time of sowing followed by two and three dosages at 20 days intervals gave significant protection to eyes with EGI of 30.55%, SK 24.07%, SCI 36.10%, StCI 30.60%, BSDI 26.43% and YR of 35.09% against the fungus which ultimately contributed to better crop stand and increased yield as compared to inoculated control and rest of the treatments.

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New components of the chitinolytic system of Trichoderma harzianum

Cited by 110 publications

References 17 publications

THE EVIDENCE OF NON N-GLYCAN LINKED MANNOSE IN EXOCHITINASE (42 kDa) FROM Trichoderma harzianum BIO10671 GLYCOSYLATION

THE EVIDENCE OF NON N-GLYCAN LINKED MANNOSE IN EXOCHITINASE (42 kDa) FROM Trichoderma harzianum BIO10671 GLYCOSYLATION

Molecular mechanisms of lytic enzymes involved in the biocontrol activity of Trichoderma harzianum

Management of Black Scurf of Potato with Effective Microbes (EM), Biological Potassium Fertilizer (BPF) and Trichoderma harzianum

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