Molecular mechanisms of lytic enzymes involved in the biocontrol activity of Trichoderma harzianum

Haran, Shoshan; Schickler, Hedva; Chet, I.

doi:10.1099/00221287-142-9-2321

Cited by 202 publications

(122 citation statements)

References 25 publications

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“…Formation of clear zones in dual cultures could be explained by the production of extracellular hydrolytic enzymes rather than production of growth inhibiting substances by Trichoderma spp. (Benhamou and Chet 1993;Haran et al, 1996). These isolates have a rapid growth compared to the other antagonists, especially the two isolates of T. hamatum.…”

Section: Discussionmentioning

confidence: 99%

Biocontrol of Wheat Fusarium Crown and Root Rot by Trichoderma spp. and Evaluation of Their Cell Wall Degrading Enzymes Activities

Dendouga¹,

Boureghda²,

Belhamra³

2016

Acta Phytopathologica et Entomologica Hungarica

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Fourteen strains of Trichoderma spp. were isolated from Algerian desert soils and assessed for their antagonistic activity against Fusarium crown and root rot of wheat. Biocontrol efficiency of Trichoderma spp. was studied by in vitro and in vivo based bioassay against three pathogenic species: F. culmorum, F. graminearum and F. verticillioides. In vitro based bioassay (dual culture) results obtained with all Trichoderma spp. isolates showed significant decrease in colony diameter of Fusarium species compared to the control. The highest percentages of reduction in colony diameter were obtained with T. harzianum Thr.4 causing a growth reduction of 70.68%, 67.05 and 70.57% against F. culmorum, F. graminearum and F. verticillioides, respectively. All Trichoderma spp. isolates were able to overgrow and sporulate above F. culmorum colonies but no overgrowth was observed with F. graminearum and F. verticilliodes. The seed treatment by Trichoderma spp. isolates before sowing in a soil already infested by the pathogens led to a significant decrease of disease severity compared to the untreated control. The highest disease index decrease (>70%) was obtained with two isolates of T. harzianum (Thr.4 and Thr.10) and T. viride Tv.6 against the three fungal pathogens. Lytic enzymes production by Trichoderma spp. isolates was tested in liquid cultures containing fungal cell walls of each pathogen as sole carbon source. Higher levels of protease and chitinase activities were induced by hyphal cell walls of F. graminearum than cell walls of F. verticillioides and F. culmorum. T. harzianum Thr.4 exhibited the highest enzyme activities with hyphal cell walls of F. graminearum and F. culmorum. However, in the medium amended with cell wall of F. verticillioides, maximal lytic activities were recorded for T. viride Tv.6.

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Section: Discussionmentioning

confidence: 99%

Biocontrol of Wheat Fusarium Crown and Root Rot by Trichoderma spp. and Evaluation of Their Cell Wall Degrading Enzymes Activities

Dendouga¹,

Boureghda²,

Belhamra³

2016

Acta Phytopathologica et Entomologica Hungarica

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“…Weindling and Emerson (1936) observed that they could excrete extracellular compound which was named gliotoxin. Many antibiotics and extracellular enzymes were isolated and characterized later, and the biocontrol mechanisms became clearer (Haran et al, 1996;Zhihe et al, 1998).…”

Section: Introductionmentioning

confidence: 99%

Antagonistic properties of Trichoderma viride on post harvest cassava root rot pathogens

Ubalua¹,

A²,

Oti³

et al. 2007

Afr. J. Biotechnol.

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The effective in vitro screening tests of Trichoderma viride for antagonism against post harvest pathogens of cassava roots (Manihot esculenta Crantz) rot together with its competitive and mycoparasitic abilities informs its selection as the most promising candidate for the biocontrol of post harvest cassava root rot pathogens. While the percentage germination of the spores were observed to increase with increasing dilution of the culture filtrate (100, 50, 25, and 10% ) at a concentration of 20,000 spores/ml, the percentage rot recorded amongst the untreated cassava roots ranged from 4% (Rhizopus oryzae) to 44% (Aspergillus flavus). Treatment with the antagonist and the subsequent storage resulted in a remarkable reduction in the frequency of occurrence of the normal root surface mycoflora and the pathogens over a 3 weeks storage period. Botryodiplodia theobromae and R. oryzae were isolated only in the first week of storage and at a frequency of 3 and 2% rot, respectively, after treatment whereas A. flavus and Fusarium solani persisted throughout the whole storage period with 2 and 3% rot on the third week, respectively. The observed results suggest that T. viride is root surface competent and highly antagonistic.

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“…Fungal protease also plays a significant role in cell wall lysis that occurs during pathogen-host interactions [44]. The highest protease activity (10 IU/ll) was expressed by the isolate CgloTIN01 and chitinase activity (4.3 IU/ll) by the isolate CgloTIN02.…”

Section: Discussionmentioning

confidence: 99%

In Vitro Evaluation of Antagonism of Endophytic Colletotrichum gloeosporioides Against Potent Fungal Pathogens of Camellia sinensis

et al. 2014

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An endophytic fungus isolated from Camellia sinensis, Assam, Northeastern India was identified as Colletotrichum gloeosporioides on the basis of morphological characteristics and rDNA ITS analysis. This endophytic fungus was evaluated for growth inhibition against tea pathogens Pestalotiopsis theae and Colletotrichum camelliae. One isolate of C. gloeosporioides showed strong antagonistic activity against Pestalotiopsis theae (64 %) and moderate activity against C. camelliae (37 %). Fifty percent cell-free culture filtrate from 5-day-old cultures showed highest antagonistic activity against both the pathogens although the inhibition percent was less as compared to dual culture. In the experiment of volatile compounds none of the isolates of C. gloeosporioides strains showed visible inhibition against P. theae and C. camelliae. The activity of extracellular hydrolytic enzymes chitinase and protease was also high in this culture fluid and measured 10 and 4.3 IU/ll, respectively.

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Molecular mechanisms of lytic enzymes involved in the biocontrol activity of Trichoderma harzianum

Cited by 202 publications

References 25 publications

Biocontrol of Wheat Fusarium Crown and Root Rot by Trichoderma spp. and Evaluation of Their Cell Wall Degrading Enzymes Activities

Biocontrol of Wheat Fusarium Crown and Root Rot by Trichoderma spp. and Evaluation of Their Cell Wall Degrading Enzymes Activities

Antagonistic properties of Trichoderma viride on post harvest cassava root rot pathogens

In Vitro Evaluation of Antagonism of Endophytic Colletotrichum gloeosporioides Against Potent Fungal Pathogens of Camellia sinensis

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