New coated SPME fibers for extraction and fast HPLC determination of selected drugs in human blood

Olszowy, Pawel; Szultka, Małgorzata; Fuchs, Patricia; Kegler, Ricarda; Mundkowski, Ralf G.; Miekisch, Wolfram; Schubert, Jochen K.; Buszewski, Bogusław

doi:10.1016/j.jpba.2010.07.002

Cited by 51 publications

(22 citation statements)

References 24 publications

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“…[19][20][21] Some organic solvents and supporting electrolytes have been used in the electropolymerization of polythiophene film and its derivatives. 15,16,20 The electrochemical properties of polythiophene films largely depend on the supporting electrolyte, especially on the type of anion. [22][23][24][25] According to Wu and Pawliszyn, 26 the use of different electrolytes enables manipulation of the functionality of the resulting polymers.…”

Section: Electropolymerization Processmentioning

confidence: 99%

“…One promising alternative for the extraction of polar and ionic compounds is the use of conductive polymers as extraction phases. 10,11 The conducting polymer coatings as polythiophene films [12][13][14][15][16] presented multifunctional properties. Thus, their polymerization on metal wires by electrochemical or chemical methods has been described as a promising alternative for the development of new phases for the SPME technique, therefore expanding the potential application of this method.…”

Section: Introductionmentioning

confidence: 99%

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Evaluation of solid-phase microextraction using a polythiophene film and liquid chromatography with spectrophotometric detection for the determination of antidepressants in plasma samples

Caris¹,

Chaves²,

Queiroz³

2012

J. Braz. Chem. Soc.

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A fase extratora de politiofeno (PTh), desenvolvida por eletropolimerização em haste de aço inox, foi avaliada para microextração em fase sólida (SPME) e análise por cromatografia líquida com detecção espectrofotométrica (LC-UV) de antidepressivos inibidores seletivos da recaptação de serotonina (citalopram, paroxetina, fluoxetina e sertralina) em amostras de plasma. A influência das variáveis da eletropolimerização (velocidade de varredura de potencial, potencial e ciclos de varredura) foi avaliada no desempenho SPME. As variáveis SPME (tempo de extração, temperatura, pH da matriz, força iônica e procedimento de dessorção), assim como a influência das proteínas do plasma no mecanismo de sorção, foram também avaliadas. O método SPME-PTh/LC-UV desenvolvido para a determinação de antidepressivos em amostras de plasma apresentou faixa linear de resposta do limite de quantificação (LOQ, 200-250 ng mL -1 ) a 4000 ng mL -1 e precisão interensaio com coeficiente de variação no intervalo de 11 a 15%. O método proposto poderá ser utilizado para a determinação de antidepressivos em amostras de plasma humano para análises toxicológicas de urgência, após a ingestão acidental ou suicida de doses altas de medicamentos.Polythiophene (PTh) phase electropolymerized on the stainless steel wire was evaluated as solidphase microextraction (SPME), and analysis by liquid chromatography with spectrophotometric detection (LC-UV) for determination of new-generation antidepressants, selective serotonin reuptake inhibitors (SSRIs) (citalopram, paroxetine, fluoxetine and sertraline), in plasma samples. The influence of electropolymerization variables (scan rate, potential range and scan cycles) was evaluated on SPME performance. The SPME variables (extraction time, temperature, matrix pH, ionic strength and desorption procedure), as well as the influence of plasma proteins on sorption mechanisms were also evaluated. The SPME/LC-UV method developed for determination of antidepressants in plasma sample presented a linear range between the limit of quantification (LOQ, 200-250 ng mL -1 ) to 4000 ng mL -1 , and interday precision with coefficient of variation (CV) ranged from 11 to 15%. The proposed method can be a useful tool for the determination of antidepressants in human plasma samples in urgent toxicological analysis after the accidental or suicidal intake of higher doses of medications.

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Section: Electropolymerization Processmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Evaluation of solid-phase microextraction using a polythiophene film and liquid chromatography with spectrophotometric detection for the determination of antidepressants in plasma samples

Caris¹,

Chaves²,

Queiroz³

2012

J. Braz. Chem. Soc.

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“…Polypyrrole and polythiophene-coated fibers have been utilized to extract antibiotics used to treat multi-resistant Staphylococcus aureus from blood 83 and adrenolytic drugs from plasma. 84 Immunoaffinity coatings, containing covalently immobilized antibodies on the surface of a fused-silica fiber, are useful for the selective and sensitive extraction of analytes.…”

Section: ·3 In-tube Spme Techniquesmentioning

confidence: 99%

Current Developments and Future Trends in Solid-phase Microextraction Techniques for Pharmaceutical and Biomedical Analyses

Kataoka

2011

ANAL. SCI.

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“…Most of these studies are directed towards biological fluid analysis by high performance liquid chromatography (HPLC), (Tobin et al, 2008;Martens-Lobenhoffer et al, 2008;Baietto et al, 2010;Gika et al, 2010;Olszowy et al, 2010;Polillo et al, 2010;Verdier et al, 2011;Szultka et al, 2013) and ultra-performance liquid chromatography (UHPLC) (Gikas et al, 2010a; Gikas et al, 2010b; Bazoti et al, 2011;Baietto et al, 2014;Naito et al, 2014). In addition, there are no monographs of this drug in any official compendium.…”

Section: Introductionmentioning

confidence: 99%

A green approach for the quantification of daptomycin in pharmaceutical formulation by UV spectrophotometry

Tótoli

Salgado

2015

Braz. J. Pharm. Sci.

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Daptomycin is the first approved drug from a new class of antimicrobials, the cyclic lipopeptides, and is a very important antimicrobial agent in current clinical practice. Currently, there are no "green" analytical methods described in the literature to analyze the typical pharmaceutical dosage form of daptomycin. Thus, the aim of this work was to validate an environment-friendly spectrophotometric method in the UV region, for the analysis of daptomycin as a lyophilized powder. Water was used as diluent and the analyses were carried out on a spectrophotometer at 221 nm. The method met all validation requirements of the ICH guidelines, over a concentration range of 6-21 µg mL -1 . A Student's t-test demonstrated that the proposed method was comparable to an HPLC method previously validated. Thus, the validated spectrophotometric method could quantify daptomycin in a powder form for injectable solutions, while being an economical, rapid, and "green" alternative for routine analysis in quality control.Uniterms: Antimicrobials/quality control. Daptomycin/determination. Spectrophotometry/UV region/ quantitative analysis. Spectrophotometry/green analytical method.A daptomicina é o primeiro membro aprovado de uma nova classe de antimicrobianos, os lipopeptídeos cíclicos, e é muito importante para a prática clínica atualmente. Não existem métodos analíticos "verdes" descritos na literatura para a análise da daptomicina na forma farmacêutica. Desta forma, o objetivo deste trabalho foi a validação de método espectrofotométrico na região do UV ambientalmente favorável para análise da daptomicina em pó liofilizado. A água foi escolhida como diluente e as análises foram realizadas em 221 nm. O método atendeu a todas as exigências de validação dos guias do ICH, na faixa de 6-21 µg mL -1 . Teste t de Student mostrou que o método proposto é intercambiável com método de HPLC previamente validado. Assim, o método espectrofotométrico validado é capaz de quantificar a daptomicina em pó para solução injetável e é uma opção econômica, rápida e "verde" para análises de rotina do controle de qualidade deste fármaco.Unitermos: Antimicrobianos/controle de qualidade. Daptomicina/determinação. Espectrofotometria/ região do UV/análise quantitativa. Espectrofotometria/método analítico verde. INTRODUCTIONIt is widely known that microbial resistance is a major problem worldwide. Many bacteria have developed resistance to a large number of antimicrobial agents available on the market; these bacteria are considered challenges for medicine. Methicillin-resistant Staphylococcus aureus (MRSA) and vancomycin resistant enterococci (VRE) are two examples of antimicrobialresistant microorganisms (Silveira et al., 2006;Wright, 2011). Compounding the problem of antimicrobial resistance, there are few new antimicrobial agents emerging in the market (Wright, 2011).One recent development in antimicrobial treatment is the introduction of daptomycin into the marketplace (Figure 1). This is the first antimicrobial agent from the cyclic lipopeptide class appro...

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New coated SPME fibers for extraction and fast HPLC determination of selected drugs in human blood

Cited by 51 publications

References 24 publications

Evaluation of solid-phase microextraction using a polythiophene film and liquid chromatography with spectrophotometric detection for the determination of antidepressants in plasma samples

Evaluation of solid-phase microextraction using a polythiophene film and liquid chromatography with spectrophotometric detection for the determination of antidepressants in plasma samples

Current Developments and Future Trends in Solid-phase Microextraction Techniques for Pharmaceutical and Biomedical Analyses

A green approach for the quantification of daptomycin in pharmaceutical formulation by UV spectrophotometry

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