New cathepsin V inhibitor from stems of Bowdichia virgilioides

Silva, Taynara L.; Fernandes, João B.; Silva, M. Fátima das G.F. da; Consolaro, Hélder; Sousa, Lorena Ramos Freitas de; Vieira, Paulo C.

doi:10.1016/j.bjp.2019.04.004

Cited by 6 publications

(8 citation statements)

References 19 publications

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“…An additional 9 extracts also partially inhibited the growth (determined by growth inhibition equal to or higher than 50 %) of any of the 8 bacteria, all from fungal cocultivation as well. Nine extracts (19,20,30,31,33,34,37,45, and 51) either totally or partially inhibited bacterial growth. In short, 60 positive results were observed (38 instances of total inhibition of bacterial growth and 22 of partial inhibition), all from cocultivation extracts.…”

Section: Resultsmentioning

confidence: 99%

“…In the study by Sarria et al [33], the activities of 2 flavonoids (luteolin and 5,2′-dihydroxy-3-methoxy-6,7-(2′,2′-dimethylchromene)-8-(3′′,3′′-dimethylallylflavanone), with IC 50 values of 2.5 and 13.5 µM, respectively were discussed. Recently, Silva et al [34] determined the cathepsin V inhibition activity of 8-methoycoumestrol (IC 50 of 17.4 ± 1.0 µM) and a trans-p-coumaric acid ester derivative (94 % inhibition at 50 µg/ mL). Clearly, the results of the antibacterial and antiprotozoal activity studies reveal that fungal cocultivation increases the chance of obtaining compounds with antimicrobial activity, since all active extracts are obtained from cocultivation.…”

Section: Resultsmentioning

confidence: 99%

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Liquid Fungal Cocultivation as a Strategy to Access Bioactive Metabolites

et al. 2020

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AbstractFungi are a rich source of bioactive compounds. Fungal cocultivation is a method of potentiating chemical interactions and, consequently, increasing bioactive molecule production. In this study, we evaluated the bactericidal, antiprotozoal, and cathepsin V inhibition activities of extracts from axenic cultures of 6 fungi (Fusarium guttiforme, Pestalotiopsis diospyri, Phoma caricae-papayae, Colletotrichum horii, Phytophthora palmivora, and C. gloeosporioides) that infest tropical fruits and 57 extracts obtained by their cocultivation. Our results reveal that fungal cocultivation enhances the biological activity of the samples, since all extracts that were active on Gram-positive bacteria, Gram-negative bacteria, Trypanosoma cruzi, and Leishmania infantum were obtained from cocultivation. Bacterial growth is either totally or partially inhibited by 46% of the extracts. Two extracts containing mainly fusaric and 9,10-dehydrofusaric acids were particularly active. The presence of the fungus F. guttiforme in co-cultures that give rise to extracts with the highest activities against L. infantum. An axenic culture gave rise to the most active extract for the inhibition of cathepsin V; however, other coculture extracts also exhibited activity toward this biological target. Therefore, the results of the biological activities indicate that fungal cocultivation increased the biological potential of samples, likely due to the hostile and competitive environment that pushes microorganisms to produce substances important for defense and allows access to metabolic routes then silenced in milder cultivation conditions.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Liquid Fungal Cocultivation as a Strategy to Access Bioactive Metabolites

et al. 2020

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“…In humans, PLCPs are known as cathepsins, and the upregulation of these enzymes in the organism is related to pathologic disorders (osteoporosis, arthritis, cancer, metastasis, etc.) [4,5]. Plants' PLCPs belong to protease family C1A of clan CA, which catalytic triad contains the amino acids Cys, His, and Asn [3].…”

Section: Introductionmentioning

confidence: 99%

Mycotoxins from Fusarium proliferatum: new inhibitors of papain-like cysteine proteases

et al. 2020

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Papain-like cysteine proteases (PLCPs) in plants are essential to prevent phytopathogen invasion. In order to search for cysteine protease inhibitors and to investigate compounds that could be associated to pineapple Fusarium disease, a chemistry investigation was performed on Fusarium proliferatum isolated from Ananas comosus (pineapple) and cultivated in Czapek medium. From F. proliferatum extracts, nine secondary metabolites were isolated and characterized by nuclear magnetic resonance spectroscopy and mass spectrometry experiments: beauvericin (1), fusaric acid (2), N-ethyl-3-phenylacetamide (3), Nacetyltryptamine (4), cyclo(L-Val-L-Pro) cyclodipeptide (5), cyclo(L-Leu-L-Pro) cyclodipeptide (6), cyclo(L-Leu-L-Pro) diketopiperazine (7), 2,4-dihydroxypyrimidine (8), and 1H-indole-3-carbaldehyde (9). Compounds 1, 3, and 6 showed significant inhibition of papain, with IC 50 values of 25.3 ± 1.9, 39.4 ± 2.5, and 7.4 ± 0.5 μM, respectively. Compound 1 also showed significant inhibition against human cathepsins V and B with IC 50 of 46.0 ± 3.0 and 6.8 ± 0.7 μM, respectively. The inhibition of papain by mycotoxins (fusaric acid and beauvericin) may indicate a mechanism of Fusarium in the roles of infection process.

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“…Another alternative strategy that has been studied in PNI is the use of natural products for their beneficial effects, such as improved motor performance, sensory function, and possible OS attenuation [ 25 ]. The bark and seeds of Bowdichia virgilioides , popularly known as sucupira-preta in Brazil, are widely used in folk medicine to treat various pathologies [ 26 ]. Our group has demonstrated the strong antioxidant effect of the hydroethanolic extract (EHE) of the stem bark of this tree both in vitro and in vivo [ 27 ].…”

Section: Introductionmentioning

confidence: 99%

Effects of Resistance Training and Bowdichia virgilioides Hydroethanolic Extract on Oxidative Stress Markers in Rats Submitted to Peripheral Nerve Injury

et al. 2020

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The objective of this study was to analyze the effects of the combination of resistance training (RT) and the hydroethanolic extract (EHE) of Bowdichia virgilioides as markers of oxidative stress (OS) in rats with peripheral nerve injury (PNI). Rats were allocated into six groups (n = 10): animals without interventions (C), animals with an exposed nerve but without injury, injured animals, trained and injured animals, injured animals that received EHE, and animals that received a combination of RT and EHE. RT comprised the climbing of stairs. EHE was orally administered (200 mg/kg) for 21 days after PNI induction. RT reduced the amount of lipoperoxidation in plasma (14.11%). EHE reduced lipoperoxidation in the plasma (20.72%) and the brain (41.36). RT associated with the extract simultaneously reduced lipoperoxidation in the plasma (34.23%), muscle (25.13%), and brain (43.98%). There was an increase in total sulhydrilyl levels (a) in the brain (33.33%) via RT; (b) in the brain (44.44%) and muscle (44.51%) using EHE; and (c) in the plasma (54.02%), brain (54.25%), and muscle using the combination of RT + EHE. These results suggest that RT associated with oral EHE results in a decrease in OS.

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New cathepsin V inhibitor from stems of Bowdichia virgilioides

Cited by 6 publications

References 19 publications

Liquid Fungal Cocultivation as a Strategy to Access Bioactive Metabolites

Liquid Fungal Cocultivation as a Strategy to Access Bioactive Metabolites

Mycotoxins from Fusarium proliferatum: new inhibitors of papain-like cysteine proteases

Effects of Resistance Training and Bowdichia virgilioides Hydroethanolic Extract on Oxidative Stress Markers in Rats Submitted to Peripheral Nerve Injury

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