New Aspects Concerning Chelating Resins

Abstract

Object of the present paper is to provide an up‐to‐date overview the application of chelating resins, a functional polymer of a special kind. Attention has been in particular limited to the field of ligand exchange chromatography, sample handling and trace enrichment, metal‐ion buffers and polymer catalysts with enzyme‐like activities. The fundamental principles in the design and syntheses of chelating resins for usage in these respects are also described.

Potentiometric Investigations into the Acid−Base and Metal Ion Binding Properties of Immobilized Metal Ion Affinity Chromatographic (IMAC) Adsorbents

“…This investigation thus provides important new information about the presence of ML 1 , ML 2 , ..., ML n and M(OH) m L 1 , ..., M(OH) m L n species as well as the determination of previously unreported equilibrium constants for different types of IMCC systems useful for protein chromatography. Additional experimental evidence based on electron spin resonance studies with transition metal ions containing unpaired d-orbital electrons 4 or solid state 13 C NMR 44 would provide confirmation of the presence of the ML n and M(OH) m L n species proposed to exist on the basis of the potentiometric data acquired for these IMCC systems. The trends evident between different im-IDA-M n+ -Sepharose CL-4B complexes in terms of stability constants were similar to those observed for the unbound metal ion-chelate complexes.…”

“…Over the past 20 years, various studies have explored the metal ion binding properties of different chelate compounds immobilized onto support materials. − In addition, comparative studies on the relative affinities of different metal ions with chelate adsorbents in the presence of various types of mobile phases have been described . However, the determination of the acid−base dissociation constants and the equilibrium constants for immobilized metal ion−chelate chromatographic (IMCC) adsorbents useful for the separation of low molecular weight solutes has only infrequently been carried out.…”

“…[1][2][3] In addition, comparative studies on the relative affinities of different metal ions with chelate adsorbents in the presence of various types of mobile phases have been described. 4 However, the determination of the acid-base dissociation constants and the equilibrium constants for immobilized metal ion-chelate chromatographic (IMCC) adsorbents useful for the separation of low molecular weight solutes has only infrequently been carried out. Where this has occurred, the investigations have usually been confined to an examination of immobilized iminodiacetic acid (IDA) systems, which were not specifically designed for use as immobilized metal ion affinity chromatographic (IMAC) adsorbents with peptides and proteins.…”