New Approach in Ulcer Prevention and Wound Healing Treatment using Doxycycline and Amoxicillin/LDH Nanocomposites

Abstract

Doxycycline (DOX) and amoxicillin (AMOX) are important Broad-spectrum antibiotics used in treating multiple human and animal diseases. For the sake of exploring novel medical applications, both antibiotics were loaded into magnesium aluminium layer double hydroxide (Mg-Al)/LDH nanocomposite through the co-precipitation method. The synthesized materials were characterized by XRD, FT-IR, particle size analysis, FESEM and HRTEM. Acute toxicological studies were conducted using median lethal dose LD 50 , where a total number of 98 rats (200–150 gm) of both sexes were used. An experimental wound was aseptically incised on the anterior-dorsal side of each rat, while 98% of pure medical ethanol was used for ulcer induction. Acute toxicity, wound closure rate, healing percentages, ulcer index, protective rate and histopathological studies were investigated. Antibiotic Nanocomposites has significantly prevented ulcer formation and improved wound healing process to take shorter time than that of the typical processes, when compared with that of same drugs in microscale systems or commercial standard drugs. These results were confirmed by the histopathological findings. By converting it into the Nanoform, which is extremely important, especially with commonly used antibiotics, novel pharmacological properties were acquired from the antibiotics. The safe uses of DOX/LDH and AMOX/LDH Nanocomposites in this study were approved for biomedical applications.

Amputation rate of diabetic foot ulcer and associated factors in diabetes mellitus patients admitted to Nekemte referral hospital, western Ethiopia: prospective observational study

“…The study conducted by Fatma I. Abo El-Ela et al showed appropriate treatment of diabetes foot infection by amoxicillin and doxycycline help to prevent bacterial growth and ulcers that heal the wounds within a short period [ 18 ]. According to the study done in China by Chu et al, for moderate/severe infection, the healing rate was rapid in patients given appropriate antibiotic therapy [ 17 ].…”

Chemisorption and sustained release of cefotaxime between a layered double hydroxide and polyvinyl alcohol nanofibers for enhanced efficacy against second degree burn wound infection

“…2, spectrum b and inset (i) show the appearance of important bands of cefotax in the spectra of the Zn-Al LDH/cefotax composite as follows: stretching of O-H groups appeared at 3456 cm À1 , N-H stretching is shown around 1569 cm À1 , C]O stretching vibration of (COO) 2À at 1852.76 cm À1 , the peaks at 1623, 1494.10, 1367.96, 1032, 772, and 610 cm À1 are due to the presence of C]O of an amide group, N-H, C]O, C-N, C-O, CH 2 , and C-S groups, respectively. 50 Two clear pieces of evidence demonstrated the successful loading of cefotax on the Zn-Al LDH host: the appearance of cefotax bands and the difference between the two spectra, which was obvious and manifested in peaks shiing toward high wavenumbers, including the -OH group peak at 3454 cm À1 shied to 3456.87 cm À1 , the bending vibration of OH peak at 1620 cm À1 shied to 1623.04 cm À1 denoting the promotion of hydrogen bonding interactions between Zn-Al LDH and cefotax, 24 C]O at 1365.67 cm À1 shied to 1367.96 cm À1 , and the M-O bond stretching at 692 shied to 610 cm À1 .…”

“…The decrease in zeta potential value reected that cefotax was extensively obtained as a successful nanocomposite formulation, not only through hydrogen bonding as referred by FTIR but also via electrostatic interaction. 24,66 3.1.5. BET and TGA-DTA analyses.…”

“…Zn-Al LDH was prepared by the co-precipitation method as follows: a solution of 0.04 mol of ZnCl 2 and 0.01 mol of AlCl 3 -$6H 2 O (with a molar ratio of 4 : 1) was added to 100 mL bidistilled water with good mixing; then NaOH (1 mol L À1 ) was added dropwise until a complete white precipitate was obtained at pH 9. The resulting suspension lasted under vigorous stirring at 50 C for 24 h, and the precipitate was collected, washed several times using bidistilled water and ethanol until pH 7, and nally dried at 40 C for 24 h. 24 The Zn-Al LDH/cefotax composite was synthesized by the aforementioned method with the same procedure. One difference was the ratio between the elemental constituents of the composite, whereby NaOH was added to a well-mixed solution of 0.04 mol of ZnCl 2 , 0.01 mol of AlCl 3 $6H 2 O, and 0.0006 mol of cefotax with a molar ration of 4 : 1 : 0.0625 ZnCl 2 : AlCl 3 $6H 2 O : cefotax, respectively.…”

“…14,15 LDHs can intercalate biologically-active molecules through anion exchange, 16,17 a property which has opened their applications in medicine and pharmacy 18 as drug carriers for many antibiotics, 16,19 anti-inammatory agents, 20 targeted drug delivery vehicles, [21][22][23] and for wound healing. 24 Recently, nanoparticles, beads, bandages, biolms, and nanobers have been considered intrinsic materials to attain sustained release. 25 In particular, nanobers are ideal candidates for wound dressing, because of their physical, mechanical, chemical properties, as well as high porosity, high surface to volume ratio, small pore size, and gas permeation properties.…”