New antigens in cells transformed by the SV40 virus. III. Presence of an SV40‐coded antigen on the surface of different cell lines transformed by this virus

Abstract

The sera of hamsters carrying tumours induced by injection of TSV5Cl2 cells and the sera of animals immunized with these cells or with SV40 itself or its purified capsids have a slow cytotoxicity activity in the presence of fresh guinea pig complement on SV40‐transformed cell strains of any origin. The responsible antigen is dependent on the SV40 genome and is associated with the cytoplasmic membranes of the transformed cells. By means of absorption of the antisera with subcellular fractions it has been correlated with antigen “C” present in the cytoplasm of SV40‐transformed cells. It is different from antigen “S” shown by the immunofluorescence test, and its relation with TSTA is discussed.