New amperometric biosensor for citrate with mercury film electrode

Hikima, Satoshi; Hasebe, Kiyoshi; Taga, Mitsuhiko

doi:10.1002/elan.1140040810

Cited by 10 publications

(3 citation statements)

References 13 publications

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“…The mercury electrode is the only liquid electrode at room temperature and its application to amperometric biosensor configuration is not common [16]. The most common types of working electrodes in amperometric biosensors are platinum, glassy carbon and gold for the anodic oxidation of hydrogen peroxide, whereas all types of carbon electrodes, e.g., graphite, carbon pastes and glassy carbon are used for the anodic oxidation of NADH and mediators.…”

Section: Electrode Materialsmentioning

confidence: 99%

Enzyme Based Amperometric Biosensors for Food Analysis

2002

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Section: Electrode Materialsmentioning

confidence: 99%

Enzyme Based Amperometric Biosensors for Food Analysis

2002

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“…Therefore, monitoring citrate content is of great importance in biomedical and analytical sciences. To date, many analytical methods have been used for citrate detection including electrochemistry [ 8 ], capillary electrophoresis [ 9 ], polarography [ 10 ], gas or liquid chromatography [ 11 , 12 ], UV-vis spectrophotometry [ 6 , 13 ], and spectrofluorimetry [ 14 , 15 ]. The spectrofluorimetry method has attracted great attention owing to its easy operation, high sensitivity, and lower equipment requirements [ 6 , 13 , 16 ].…”

Section: Introductionmentioning

confidence: 99%

A Novel “Off-On” Fluorescent Probe Based on Carbon Nitride Nanoribbons for the Detection of Citrate Anion and Live Cell Imaging

Yang

et al. 2018

Sensors

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A novel fluorescent “off-on” probe based on carbon nitride (C3N4) nanoribbons was developed for citrate anion (C6H5O73−) detection. The fluorescence of C3N4 nanoribbons can be quenched by Cu2+ and then recovered by the addition of C6H5O73−, because the chelation between C6H5O73− and Cu2+ blocks the electron transfer between Cu2+ and C3N4 nanoribbons. The turn-on fluorescent sensor using this fluorescent “off-on” probe can detect C6H5O73− rapidly and selectively, showing a wide detection linear range (1~400 μM) and a low detection limit (0.78 μM) in aqueous solutions. Importantly, this C3N4 nanoribbon-based “off-on” probe exhibits good biocompatibility and can be used as fluorescent visualizer for exogenous C6H5O73− in HeLa cells.

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“…Although relatively slow and expensive in enzyme the approach works well, as a batch method, and is widely used in routine off-line analysis. Since then several other approaches have been proposed; some make use of soluble CL and immobilized MDH with NADH 10 detection, some use CL and oxaloacetate decarboxylase (OACD) in soluble 11 or immobilized 12 form in conjunction with polarography. The direct amperometric determination of citric acid has been proposed 13 in connection with an ascorbate oxidase reactor to eliminate ascorbic acid interference.…”

mentioning

confidence: 99%

Bioelectrochemical Determination of Citric Acid in Real Samples Using a Fully Automated Flow Injection Manifold

Tzouwara-Karayanni¹,

Karayannis²,

Prodromidis³

et al. 1997

Analyst

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An enzymic method for the determination of citric acid in fruits, juices and sport drinks is proposed. The method is based on the action of the enzymes citrate lyase, oxaloacetate decarboxylase and pyruvate oxidase, which convert citric acid into H2O2 with the latter being monitored amperometrically with a H2O2 probe. The enzymes pyruvate oxidase and oxaloacetate decarboxylase were immobilized. A multi-membrane system, consisting of a cellulose acetate membrane for the elimination of interferants, an enzymic membrane and a protective polycarbonate membrane were placed on a Pt electrode and used with a fully automated flow injection manifold. Several parameters were optimized, resulting in a readily constructed and reproducible biosensor. Interference from various compounds present in real samples was minimized. Calibration graphs were linear over the range 0.01-0.9 mM pyruvate, 0.015-0.6 mM oxaloacetate and 0.015-0.5 mM citrate. The throughput was 30 samples h-1 with an RSD of 1.0% (n = 8); the mean relative error was 2.4% compared with a standard method. The recovery was 96-104%. A 8-10% loss of the initial activity of the sensor was observed after 100-120 injections.

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New amperometric biosensor for citrate with mercury film electrode

Cited by 10 publications

References 13 publications

Enzyme Based Amperometric Biosensors for Food Analysis

Enzyme Based Amperometric Biosensors for Food Analysis

A Novel “Off-On” Fluorescent Probe Based on Carbon Nitride Nanoribbons for the Detection of Citrate Anion and Live Cell Imaging

Bioelectrochemical Determination of Citric Acid in Real Samples Using a Fully Automated Flow Injection Manifold

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