Bioelectrochemical Determination of Citric Acid in Real Samples Using a Fully Automated Flow Injection Manifold

Tzouwara-Karayanni, Stella M.; Karayannis, Miltiades I.; Prodromidis, Mamas I.; Vadgama, P.

doi:10.1039/a702312j

Cited by 40 publications

(16 citation statements)

References 18 publications

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“…Calibration graphs were linear over the range 0.01-0.9 mM of pyruvate, 0.015-0.6 mM of oxaloacetate, and 0.015-0.5 mM of citrate. A 8-10% loss of the initial activity of the biosensor was observed after 100-120 injections (Prodromidis et al 1997). Maines et al coimmobilized (or immobilized separately) these enzymes on different types of high protein binding membrane such as mixed cellulose ester.…”

Section: Citric Acidmentioning

confidence: 97%

Application of Enzyme Biosensors in Analysis of Food and Beverages

et al. 2011

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The importance of analyses of different parameters in food products and monitoring of a production process requires quick and reliable analytical methods and devices. For this purpose, biosensors can be a suitable option, whereas most of the current quality control techniques are time consuming, expensive, and unpractical. In this paper, we describe biosensors developed for analysis of different components present in food samples, namely, glucose, fructose, sucrose, lactose, lactic, malic, acetic, ascorbic, citric and amino acids, ethanol, glycerol, and triglyceride. Biosensors showed desirable sensitivity, selectivity, and response time required for various applications. They are often designed to avoid interference from components present in a complex sample to be analyzed.

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Section: Citric Acidmentioning

confidence: 97%

Application of Enzyme Biosensors in Analysis of Food and Beverages

et al. 2011

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“…Pyruvate oxidase is a relatively unstable enzyme, probably due to the gradual loss of its cofactor flavin adenine dinucleotide (FAD), during the immobilization procedure and during the measurements [39]. The enzyme electrodes long-term stability was tested over a period of two weeks by performing one calibration curve each day.…”

Section: Compoundmentioning

confidence: 99%

Development of Novel Glucose and Pyruvate Biosensors at Poly(Neutral Red) Modified Carbon Film Electrodes. Application to Natural Samples

Ghica

Brett

2006

Electroanalysis

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Amperometric biosensors based on the corresponding oxidase enzyme with poly(neutral red) redox mediator have been developed for the determination of glucose and pyruvate. The enzymes have been immobilized on top of poly(neutral red) modified carbon film electrodes with glutaraldehyde as the cross-linking agent. The biosensors were characterized by cyclic voltammetry and by electrochemical impedance spectroscopy. The glucose biosensor exhibited a linear response in the range 90 mM to 1.8 mM with a detection limit of 22 mM and the pyruvate biosensor in the range 90 to 600 mM with a detection limit of 34 mM. The relative standard deviations were found to be 2.1% (n ¼ 3) and 2.8% (n ¼ 4) respectively. The interference effects of various compounds were also studied. The glucose content of several types of wine and the amount of pyruvate in onion and garlic were determined and the results were compared with those obtained by standard spectrophotometric methods.

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“…Os teores médios de ácido cítrico e ascórbico encontrados nas cajuínas (Tabela 4) estão na mesma ordem de grandeza em relação aos encontrados para outros tipos de bebidas e produtos alimentares (refrigerantes, sucos de frutas, frutas, vegetais, etc.) [12,13,17,22,27].…”

Section: -Aldeídos Furânicos áCidos E Açúcares Em Amostras De Cajuínasunclassified

Determinação de aditivos, aldeídos furânicos, açúcares e cafeína em bebidas por cromatografia líquida de alta eficiência: validação de metodologias

Aquino

Amorim

Prata³

et al. 2004

Ciênc. Tecnol. Aliment.

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Avaliação das metodologias para determinação de aldeido furânico e cafeína em produto alimentício, Nascimento et al. 11/10/2001. Aceito para publicação em 06/11/2003 (000757). 2. Departamento de Química Analítica e Físico Química (DQAFQ), Centro de Ciências, Br. *ronaldo@ufc.br. 3 Recebido para publicação em . Laboratório de Análise de Bebidas e Vinagre (LABV-CE) -Ministério daAgricultura e Abastecimento -Ceará. *A quem a correspondência deve ser enviada. -INTRODUÇÃOOs aditivos, segundo a legislação brasileira [5, 7], são "substâncias intencionalmente adicionadas aos alimentos com o objetivo de conservar, intensificar ou modificar suas propriedades, desde que não prejudiquem seu valor nutritivo". Esta mesma legislação obriga os produtores a mencionarem nas embalagens de alimentos e bebidas os aditivos utilizados [5, 7] e também proíbe que os aditivos substituam a matéria-prima básica, alterem o valor nutritivo, sejam tóxicos ou mascarem falhas de fabricação. Muitos aditivos utilizados em alimentos e bebidas apresentam propriedades toxicológicas. Portanto, é dever dos órgãos competentes fiscalizar a qualidade dos alimentos industrializados para preservar a saúde dos consumidores.No Brasil, as metodologias oficiais disponíveis para a análise de aditivos, cafeína, aldeídos furânicos, ácido ascórbico e açúcares são pouco sensíveis, pouco confiá-veis, não seletivas e morosas [5,7,19,26] quando comparadas aos métodos cromatográficos [1,2,9,12,13,14,18,20,21,22,23,24]. Fatores como estes as tornam obsoletas requerendo a implementação de métodos mais sensíveis, rápidos e passíveis de automação. Os métodos oficiais executados pelo laboratório vinculado ao Ministério da Agricultura e Abastecimento-Ceará para a determinação de cafeína, ácido ascórbico e açúcares são exemplos notórios da morosidade de suas metodologias [5, 7]. The study has been evidenced that 66% of the cajuinas, present low quality because of the process of heating, and 12% of the soft drinks presented content of sodium benzoate above the limit tolerated by Brazilian legislation and 29% of the soft drinks illegal saccharin addition. The methodologies were validated in function of the precision, experimental recovery, linearity of the analytical curves, limit of detection and quantification for each compound studied. The precision (R.S.D%) of the determination of each compound varied of 2.85 to 8.35 % and recovery was between 95,1 to 105,5 %. The linearity of the analytical curves were satisfactory with coefficients of correlation greater than 0.990. The limit of detection and quantification of each compound were obtained in level of µg/L.

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Bioelectrochemical Determination of Citric Acid in Real Samples Using a Fully Automated Flow Injection Manifold

Cited by 40 publications

References 18 publications

Application of Enzyme Biosensors in Analysis of Food and Beverages

Application of Enzyme Biosensors in Analysis of Food and Beverages

Development of Novel Glucose and Pyruvate Biosensors at Poly(Neutral Red) Modified Carbon Film Electrodes. Application to Natural Samples

Determinação de aditivos, aldeídos furânicos, açúcares e cafeína em bebidas por cromatografia líquida de alta eficiência: validação de metodologias

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