Development of Novel Glucose and Pyruvate Biosensors at Poly(Neutral Red) Modified Carbon Film Electrodes. Application to Natural Samples

Ghica, Mariana Emilia; Brett, Christopher M.A.

doi:10.1002/elan.200503468

Cited by 95 publications

(71 citation statements)

References 42 publications

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“…Poly(neutral red) (PNR) has been extensively used as mediator for the construction of various biosensor assemblies, for glucose, pyruvate and alcohol detection [13,14]. A biosensor was developed for use in flow analysis for glucose monitoring, containing the enzyme entrapped in a sol-gel layer on a PNR-modified carbon film electrode [15].…”

Section: E-mail Address: Brett@ciucpt (Cma Brett)mentioning

confidence: 99%

Electrosynthesis and electrochemical characterisation of phenazine polymers for application in biosensors

Bârsan

Pinto

Brett

2008

Electrochimica Acta

106

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A comparative investigation has been undertaken of the electrosynthesis and electrochemical properties of three different electroactive polymers on carbon film electrode substrates: poly(neutral red) from the phenazine dye neutral red, and poly(methylene green) and poly(methylene blue), from the corresponding phenothiazine dyes. The formation of the radical cation at different potentials and the chemical structures of the monomers both influence the electropolymerisation process of the three polyaromatic dyes. Of the three, poly(neutral red) is shown to have the best adhesion at carbon film electrodes. The influence of the electrolyte and pH on film growth and on electrochemical properties was investigated. The formal potential decreased linearly with increase in pH, in the pH range from 1 to 7 for all three polymers. The modified electrodes were also characterised by electrochemical impedance spectroscopy. The bulk and interfacial characteristics of the two phenothiazine polymers were similar and oxygendependent, but different to those of the phenazine polymer, poly(neutral red), which were not significantly influenced by the presence of oxygen in solution. Perspectives for use in electrochemical biosensors are indicated.

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Section: E-mail Address: Brett@ciucpt (Cma Brett)mentioning

confidence: 99%

Electrosynthesis and electrochemical characterisation of phenazine polymers for application in biosensors

Bârsan

Pinto

Brett

2008

Electrochimica Acta

106

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“…Neutral red (NR), an acid base indicator with pK a ~6.8 from the family of azines, can be electropolymerised and poly(neutral red) (PNR) has already been used as redox mediator in enzyme electrodes [17,22]. The combination of different polymers to obtain Page 4 of 29 A c c e p t e d M a n u s c r i p t 4 composites with better properties than each polymer by itself is of current interest [13].…”

Section: Introductionmentioning

confidence: 99%

Preparation and characterisation of poly(3,4-ethylenedioxythiophene) and poly(3,4-ethylenedioxythiophene)/poly(neutral red) modified carbon film electrodes, and application as sensors for hydrogen peroxide

Gonçalves

Ghica

Brett

2011

Electrochimica Acta

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Please cite this article as: A.R. Gonçalves, M.E. Ghica, C.M.A. Brett, Preparation and characterisation of poly (3,4-ethylenedioxythiophene) and poly(3,4-ethylenedioxythiophene)/poly(neutral red) modified carbon film electrodes; and application as sensors for hydrogen peroxide, Electrochimica Acta (2010Acta ( ), doi:10.1016Acta ( /j.electacta.2010 This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. carbon-film electrodes (CFE) in aqueous solution using electropolymerisation by potential cycling, potentiostatically and galavanostatically. Characterisation of the modified electrodes was done by cyclic voltammetry and electrochemical impedance spectroscopy and the stability of the polymer films was probed. The coated electrodes were tested for application as hydrogen peroxide sensors, by oxidation and reduction. A novel polymer film was also formed by modification of CFE by co-electropolymerisation of EDOT and the phenazine dye neutral red (NR) -(PEDOT/PNR) with a view to enhancing the properties for sensor applications. It was found that hydrogen peroxide reduction at the PEDOT/PNR coated electrodes could be carried out at a less negative potential, the sensor performance comparing very favourably with that of other polymer-modified electrodes reported in the literature.

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“…Glutaraldehyde has usually been used as a protein cross-linking agent in biosensor fabrication using carbon-film electrodes [8][9][10][11][12][13]. Biosensors for glucose determination have been reported using ferrocene [9], CoHCF [10], methyl viologen [11] and PNR [12] as redox mediators, and multienzyme sensors have been used for analysis of various foodstuffs [13]. It is a bifunctional cross-linking agent which reacts with lysine residues on the exterior of the proteins.…”

Section: E-mail Address: Brett@ciucpt (Cma Brett)mentioning

confidence: 99%

“…The potential was cycled from −1.0 to 1.0 V versus Ag/AgCl at a scan rate 50 mV s −1 for 15 cycles [12].…”

Section: Electrode and Mediator Film Preparationmentioning

confidence: 99%

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Design and application of a flow cell for carbon-film based electrochemical enzyme biosensors

Bârsan

Klincar

Batič

et al. 2007

Talanta

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A flow cell has been designed for use with an electrochemical enzyme biosensor, based on low-cost carbon-film electrodes. Three types of mediators were used: cobalt and copper hexacyanoferrates and poly(neutral red) (PNR), covered with glucose oxidase (GOx) immobilised by cross-linking with glutaraldehyde in the presence of bovine serum albumin or inside a oxysilane sol-gel network. Mixtures of sol-gel precursors were made from 3-aminopropyl-triethoxysilane (APTOS) together with methyltrimethoxysilane (MTMOS), methyltriethoxysilane (MTEOS), tetraethyloxysilane (TEOS) or 3-glycidoxypropyl-trimethoxysilane (GOPMOS), and the best chosen for encapsulation. Optimisation in batch mode, using amperometric detection at fixed potential, showed the PNR-GOx modified carbon-film electrodes to be best for flow analysis for both glutaraldehyde and sol-gel enzyme immobilisation. Both types of enzyme electrode were tested under flow conditions and the reproducibility and stability of the biosensors were evaluated. The biosensors were used for fermentation monitoring of glucose in grape must and interference studies were also performed.

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Development of Novel Glucose and Pyruvate Biosensors at Poly(Neutral Red) Modified Carbon Film Electrodes. Application to Natural Samples

Cited by 95 publications

References 42 publications

Electrosynthesis and electrochemical characterisation of phenazine polymers for application in biosensors

Electrosynthesis and electrochemical characterisation of phenazine polymers for application in biosensors

Preparation and characterisation of poly(3,4-ethylenedioxythiophene) and poly(3,4-ethylenedioxythiophene)/poly(neutral red) modified carbon film electrodes, and application as sensors for hydrogen peroxide

Design and application of a flow cell for carbon-film based electrochemical enzyme biosensors

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