New aminoglycoside-modifying enzymes APH(3′)-VIII and APH(3′)-IX in Acinetobacter rudis and Acinetobacter gerneri

Abstract

Analysis of whole-genome sequences of 133 strains of Acinetobacter detected two genes for new types of aminoglycoside 3′-O-phosphotransferase [APH(3′)], type VIII in Acinetobacter rudis and IX in A. gerneri. The enzymes were related to each other (49% identity) and to APH(3′)-VI (61% and 51% identity, respectively), which is intrinsic to A. guillouiae. The cloned genes conferred kanamycin and amikacin resistance to Escherichia coli but were cryptic or expressed at low levels in the original hosts. The chromosomal location of both genes and the genetic events for acquisition of an ancestral aphA gene by A. rudis and A. gerneri, and loss by A. bereziniae were supported by the molecular phylogenetic tree of these genes. These data confirm that nonpathogenic susceptible bacterial species can be considered as potential reservoirs of resistance genes.

A Correlational Analysis of the Phosphate Solubilising Bacteriathe Growth Rate of Lady Rosetta Potato

“…In addition, a poor negative correlation (r=0.157 f=0.049 at 0.00) was found between the average Acinetobacter rudis maximal colonization and maximal enhanced effect on plant growth parameters and nutrient uptake. 22,23 There is a direct effect of root exudates on the colonization behaviours of specific bacterial strains, as well as on the type of plant. 24 The presence of antigenic lipopolysaccharide (LPS) molecules in phosphate attachment to roots mediated the interaction with root cells to enhance root growth.…”

Antibiotic resistance: turning evolutionary principles into clinical reality

“…In the environmental niche, a number of factors are known to exert strong selective pressure for acquiring resistance to antibiotics [ Figure 1., (Yoon, et al, 2016, Yoon, et al, 2017]. These include natural production of antibiotics by microbes, influx of human-generated waste enriched for excreted antibiotics (depending on the antibiotic class, up to 80% of an antibiotic administered to humans is excreted via urine in an unchanged chemical form, the most stable classes being glycopeptides, aminoglycosides and fluoroquinolones), influx of antibiotics used in animal production and aquaculture, and pollution from pharmaceutical manufacturing.…”

Complete Genome Sequencing of Acinetobacter sp. Strain LoGeW2-3, Isolated from the Pellet of a White Stork, Reveals a Novel Class D Beta-Lactamase Gene

“…Acinetobacter species found in diverse environmental habitats are considered to contribute to the mobilization of antibiotic resistance genes into clinically relevant Acinetobacter species ( 7 – 9 ). A search for putative resistance genes in the genome of Acinetobacter sp.…”