Objective: To determine the effect of Acinetobacter rudis-S23 as a Phosphorus-Solubilizing Bacterium (PSB) on potato growth. Methods: A field experiment was conducted in two growing seasons, 2019/2020 and 2020/2021, using a Randomized Complete Block Design (RCBD) with three replications. The treatment group applied A. rudis-S23 as a Phosphate-Solubilizing Bacterium (PSB) to the soil, whereas the control group was untreated. Findings: The application of A. rudis-S23 resulted in a significant improvement in various plant growth metrics compared to untreated plants. Specifically, PSB-treated plants exhibited an 18% increase in plant height, a 38% increase in branch number, and a 29% increase in shoot dry weight. The availability of phosphorus in the soil was significantly enhanced by the application of PSB, showing an increase of up to 57% compared to the untreated. Potato tuber absorption of nutrients also saw a substantial improvement, with a 74% increase in tuber nutrient uptake noted in the PSB-treated plants. Novelty: This report signifies the pioneering field study of potato plants employing the mangrove endophytic bacterium A. rudis-S23 as a phosphate solubilizer. This bacterium was originally isolated from an unexplored region within the Kutch, Gujarat, India, marking a significant contribution to the scientific understanding of this unique microbial resource.