Neuroprotective effect of acute melatonin treatment on hippocampal neurons against irradiation by inhibition of caspase-3

Li, Jianguo; Zhang, Guowei; Zhang, Meng; Wang, Lingzhan; Liu, Haiying; Liu, Qiang; Batu, Buren

doi:10.3892/etm.2016.3215

Cited by 9 publications

(12 citation statements)

References 42 publications

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“…Another interesting finding was the observed increase in caspase-3 (CASP3) mRNA in response to all treatments in both MCF7 and MDA-MB-231 cells. Modulation of caspase-3 mRNA was previously reported in normal hippocampal neurons of rats treated with melatonin prior to irradiation, where the neuroprotective effect of melatonin associated with a reduction in both caspase-3 mRNA and caspase-3 protein cleavage [59]. To the best of our knowledge, ours is the first study to assess the effect of melatonin on caspase-3 mRNA in cancer cells, as most studies examined levels of cleaved caspase-3 protein.…”

Section: Discussionmentioning

confidence: 65%

Efficacy of melatonin, IL-25 and siIL-17B in tumorigenesis-associated properties of breast cancer cell lines

et al. 2017

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Mammary tumorigenesis can be modulated by melatonin, which has oncostatic action mediated by multiple mechanisms, including the inhibition of the activity of transcription factors such as NF-κB and modulation of interleukins (ILs) expression. IL-25 is an active cytokine that induces apoptosis in tumor cells due to differential expression of its receptor (IL-17RB). IL-17B competes with IL-25 for binding to IL-17RB in tumor cells, promoting tumorigenesis. This study purpose is to address the possibility of engaging IL-25/IL-17RB signaling to enhance the effect of melatonin on breast cancer cells. Breast cancer cell lines were cultured monolayers and 3D structures and treated with melatonin, IL-25, siIL-17B, each alone or in combination. Cell viability, gene and protein expression of caspase-3, cleaved caspase-3 and VEGF-A were performed by qPCR and immunofluorescence. In addition, an apoptosis membrane array was performed in metastatic cells. Treatments with melatonin and IL-25 significantly reduced tumor cells viability at 1mM and 1ng/mL, respectively, but did not alter cell viability of a non-tumorigenic epithelial cell line (MCF-10A). All treatments, alone and combined, significantly increased cleaved caspase-3 in tumor cells grown as monolayers and 3D structures (p<0.05). Semi-quantitative analysis of apoptosis pathway proteins showed an increase of CYTO-C, DR6, IGFBP-3, IGFBP-5, IGFPB-6, IGF-1, IGF-1R, Livin, P21, P53, TNFRII, XIAP and hTRA proteins and reduction of caspase-3 (p<0.05) after melatonin treatment. All treatments reduced VEGF-A protein expression in tumor cells (p<0.05). Our results suggest therapeutic potential, with oncostatic effectiveness, pro-apoptotic and anti-angiogenic properties for melatonin and IL-25-driven signaling in breast cancer cells.

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Section: Discussionmentioning

confidence: 65%

Efficacy of melatonin, IL-25 and siIL-17B in tumorigenesis-associated properties of breast cancer cell lines

et al. 2017

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“…Additionally, melatonin was reported to prevent neurotoxicity induced by cadmium in mouse neuroblastoma cells, via the activation of transcription factor EB-dependent autophagy-lysosome [ 136 ]. Moreover, the beneficial effects of melatonin against irradiation was observed after melatonin was administered at 100 mg/kg in male Sprague-Dawley rats, due to the caspase-3 inhibition [ 307 ]. In addition, melatonin (50 mg/kg) was found to prevent the neuronal damage in the hippocampus in pregnant rats caused by 900 MHz electromagnetic fields (EMF), which might be associated with the use of mobile phones [ 308 ].…”

Section: Bioactivities Of Melatoninmentioning

confidence: 99%

Dietary Sources and Bioactivities of Melatonin

et al. 2017

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Insomnia is a serious worldwide health threat, affecting nearly one third of the general population. Melatonin has been reported to improve sleep efficiency and it was found that eating melatonin-rich foods could assist sleep. During the last decades, melatonin has been widely identified and qualified in various foods from fungi to animals and plants. Eggs and fish are higher melatonin-containing food groups in animal foods, whereas in plant foods, nuts are with the highest content of melatonin. Some kinds of mushrooms, cereals and germinated legumes or seeds are also good dietary sources of melatonin. It has been proved that the melatonin concentration in human serum could significantly increase after the consumption of melatonin containing food. Furthermore, studies show that melatonin exhibits many bioactivities, such as antioxidant activity, anti-inflammatory characteristics, boosting immunity, anticancer activity, cardiovascular protection, anti-diabetic, anti-obese, neuroprotective and anti-aging activity. This review summaries the dietary sources and bioactivities of melatonin, with special attention paid to the mechanisms of action.

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“…In addition, the band density of caspase‐3 was normalized to the related β‐actin band (internal control). Ultimately, densitometry software (Image J‐1.44) was used to quantify the protein bands (Ehsanifar et al, 2019; Li et al, 2016).…”

Section: Methodsmentioning

confidence: 99%

Neuroprotective effect of melatonin on radiation‐induced oxidative stress and apoptosis in the brainstem of rats

Motallebzadeh

Tameh

Talaei

et al. 2020

Journal Cellular Physiology

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This study aimed to determine the effects of melatonin on irradiation‐induced apoptosis and oxidative stress in the brainstem region of Wistar rats. Therefore, the animals underwent whole‐brain X‐radiation with a single dose of 25 Gy in the presence or absence of melatonin pretreatment at a concentration of 100 mg/kg BW. The rats were allocated into four groups (10 rats in each group): namely, vehicle control (VC), 100 mg/kg of melatonin alone (MLT), irradiation‐only (RAD), and irradiation plus 100 mg/kg of melatonin (RAM). An hour before irradiation, the animals received intraperitoneal (IP) melatonin and then were killed after 6 hr, followed by measurement of nitric oxide (NO), malondialdehyde (MDA), superoxide dismutase (SOD), glutathione peroxidase (GPx), catalase (CAT), and total antioxidant capacity (TAC) in the brainstem region. Furthermore, the western blot analysis technique was performed to assess the caspase‐3 expression level. Results showed significantly higher MDA and NO levels in the brainstem tissues for the RAD group when compared with the VC group (p < .001). Moreover, the irradiated rats exhibited a significant decrease in the levels of CAT, SOD, GPx, and TAC (p < .01, p < .001, p < .001, and p < .001, respectively) in comparison to the VC group. The results of apoptosis assessment revealed that the expression level of caspase‐3 significantly rose in the RAD group in comparison with the VC group (p < .001). Pretreatment with melatonin ameliorated the radiation‐induced adverse effects by decreasing the MDA and NO levels (p < .001) and increasing the antioxidant enzyme activities (p < .001). Consequently, the caspase‐3 protein expression level in the RAM group showed a significant reduction in comparison with the RAD group (p < .001). In conclusion, melatonin approximately showed a capacity for neuroprotective activity in managing irradiation‐induced oxidative stress and apoptosis in the brainstem of rats; however, the use of melatonin as a neuroprotective agent in humans requires further study, particularly clinical trials.

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Neuroprotective effect of acute melatonin treatment on hippocampal neurons against irradiation by inhibition of caspase-3

Cited by 9 publications

References 42 publications

Efficacy of melatonin, IL-25 and siIL-17B in tumorigenesis-associated properties of breast cancer cell lines

Efficacy of melatonin, IL-25 and siIL-17B in tumorigenesis-associated properties of breast cancer cell lines

Dietary Sources and Bioactivities of Melatonin

Neuroprotective effect of melatonin on radiation‐induced oxidative stress and apoptosis in the brainstem of rats

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