Neuromedin U Type 1 Receptor Stimulation of A-type K+ Current Requires the βγ Subunits of Go Protein, Protein Kinase A, and Extracellular Signal-regulated Kinase 1/2 (ERK1/2) in Sensory Neurons

Abstract

Background: Regulation of I A has an important role in determining the electrical properties of dorsal root ganglion neurons. Results: Neuromedin U (NMU) increases I A via NMUR1 that couples to the ␤␥ subunits of G o protein, PKA, and ERK. Conclusion: NMUR1 stimulation of I A contributes to neuronal hypoexcitability. Significance: Selective regulation of I A by G protein-coupled receptors will contribute to neuronal excitability and subsequent transmission of the nociceptive electrical signals.

Neuromedin S Increases L-type Ca2+Channel Currents Through Gia-protein and Phospholipase C-dependent Novel Protein Kinase C Delta Pathway in Adult Rat Ventricular Myocytes

“…In addition, we show in this study that NMUR2 was expressed in adult rat ventricular myocytes, whereas NMUR1 expression could not be detected. These receptor distribution patterns have started to allow the assignment of particular physiological roles of NMS and its receptors in mammalians [6,20,26]. By using genetic model, previous studies have shown that activation of NMUR1 inhibited L-type Ca 2+ channel currents in mouse hippocampal neurons [26].…”

“…Western blot analysis was performed following the procedure described by previous reports [18][19][20]. For antibody detection, after incubated with 5% non-fat milk in TBST for 1 h at room temperature, membranes were then incubated with diluted primary antibody goat anti-rat NMUR1 (Santa Cruz, 1:500) or NMUR2 (Santa Cruz, 1:500) and incubated at 4 °C overnight.…”

“…QEHA (200 µM), a synthetic peptide, which competitively binds G βγ and blocks G βγ -mediated signaling [20,21], was introduced into the recording pipette (Fig. 5A).…”

Urotensin-II Receptor Stimulation of Cardiac L-type Ca2+ Channels Requires the βγ Subunits of Gi/o-protein and Phosphatidylinositol 3-Kinase-dependent Protein Kinase C β1 Isoform

“…Western Blotting-Western blot analysis was performed as described previously (30,31). Briefly, total cellular protein was extracted from rat ventricular myocytes using homogenization buffer.…”

Nesfatin-1 Suppresses Cardiac L-type Ca2+ Channels Through Melanocortin Type 4 Receptor and the Novel Protein Kinase C Theta Isoform Pathway

“…Western blot analysis was performed as previously described [17,24,25]. In brief, cells were homogenized by sonication at 4 °C in homogenization buffer containing protease inhibitors.…”