Neural control of periodic secretion of the pancreas and the stomach in fasting dogs.

Abstract

SUMMARY1. The role of nerves in periodic secretion of the pancreas and the stomach in relation to the motility of the upper gastrointestinal tract was studied in conscious fasting dogs which had previously been provided with chronic gastric and pancreatic fistulae and a Heidenhain pouch.2. Both atropine and pentolinium abolished the periodic increase in gastric and pancreatic secretion and motility of the gut.3. Bilateral cervical vagal blockade with lidocaine reduced the motility of the stomach, the duodenum and the pouch preceding their peaks, but the motility at the peaks remained unchanged except in the case of the stomach.4. Pancreatic secretion preceding its peak was also decreased by vagal blockade but that at the peak was not significantly different from the control peak.5. Periodic pepsin secretion, from both the fistula and pouch, was decreased by vagal blockade.6. It is concluded that the secretion and motility of the upper gut in fasting dogs is controlled by periodic activity of the vagus and intrinsic nerves.

Collection of pancreatic juice in experimental animals: mini-review of materials and methods

“…In this method, the animal was kept in a metabolic cage, and a persistaltic pump pumped the juice back into the animal at a low constant speed. Adequate pumping rate is difficult to assess, since pancreatic secretion shows marked circadian oscillations (Magee and Naruse, 1983;Onaga et al, 1993;Zabielski et al, 1993). Therefore the dead space of the tubings and the retention container is large, ie, about 5 to 20% of daily secretion.…”

Gastrin, cholecystokinin and gastrointestinal tract functions in mammals

“…Moreover, stimulation with a low dose of CCK can be abolished by atropine or cold blockade of the vagal nerves, suggesting involvement of neural pathways. Magee & Naruse (1983 explained this by an interplay between the nervous and hormonal regulation systems at the level of intrapancreatic nerves and ganglions. However, studies comparing the secretory responses to CCK8 administered into the general circulation or locally into a branch of the right gastroepiploic artery, which supplies the proximal duodenum (but not the pancreas), showed more pronounced responses following the intra-arterial infusions in both conscious and anaesthetised animals Zabielski et al 1991Zabielski et al , 1994Kiela et al 1996).…”

Comparison of the action of cholinomimetics and pentagastrin on gastric secretion in dogs

“…Total acid and~2 pepsin secreted in the 100min following peak secretions were compared also by analysis of variance. In our animals 100 min was found to be the duration of one complete interdigestive cycle (Magee & Naruse, 1983). Pentolinium tartrate (Wyeth Laboratories, Inc., Philadelphia, Pa.), a ganglionic blocking agent, was given (1 mg kg-') intramuscularly.…”