Collection of pancreatic juice in experimental animals: mini-review of materials and methods

Zabielski, R.; Leśniewska, V.; Guilloteau, Paul

doi:10.1051/rnd:19970401

Cited by 9 publications

(7 citation statements)

References 26 publications

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“…Dursun et al (18) reported that the gastroduodenal vein formed with the cranial pancreaticoduodenal vein, which was divided into the corpus and right pancreatic lobes (5). In the present study, the cranial pancreaticoduodenal vein was seen to divide to the right side of the duodenal and gastric lobes in the rat pancreas.…”

Section: Resultssupporting

confidence: 62%

“…Obtaining information on the exact location of the vessels, nerves and ducts of the pancreas are crucial for the collection of pancreatic juice (5), experimental acute pancreatitis (6), cannula practices, ligation of the common biliary duct or pancreatic duct (7), and placement of the ligation (8). The surrounding structure of the pancreas is well documented in several textbooks and studies, however there is a lack of topographic anatomy (9).…”

Section: Introductionmentioning

confidence: 99%

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Morphological aspects of the pancreas in the rat and the rabbit: an investigation into the location, ducts, arteries and veins

Haligür

Karakurum

Dilek

2018

Veterinary Journal of Mehmet Akif Ersoy University

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The aim of the present study was to determine both the location of the pancreatic duct and the anatomical variation in the pancreatic ducts of rat and rabbit. Following administration of ether anesthesia, the abdomens were opened. Catheters were placed in the ileocolic vein, junction of the duodenum and jejunum, and into the aorta. All arteries, veins and pancreatic ducts were filled with red, blue and white dyed latex, respectively. The rat pancreas was consisted of three lobes, while the rabbit pancreas was consisted of two lobes. The rat pancreas also involved a diffuse gland situated in the gastrolienal ligament and mesoduodenum. It was observed two major ducts which name posterior pancreatic duct, anterior pancreatic ducts. The ducts that were determined in the right side of the biliaropancreatic duct open from origin the biliaropancreatic duct were measurements 8.09+2.65 mm right side and left side 7.32+3.61 mm. The rabbit pancreas included a diffuse gland situated among the liver, stomach and duodenum. In the rabbit, the duct of the pancreas (pancreatic duct) entered the duodenum 51.52+3.23cm distal to the pylorus. Bunting & Jones (19) reported that the pancreatic duct opened to the duodenum and 25-27cm away from (distal part) the pylorus in rabbit, the present authors found the pancreatic duct opened to the duodenum and measured 46.33-57.17cm in our study. Tavşan ve rat pankreas'ının morfolojik görünümü: Lokalizasyonu, kanalları, arterleri ve venleri üzerine bir araştırma ÖZ Bu çalışmanın amacı rat ve tavşanlarda ductus pancreaticus'un yerleşimini ve bu türlerde ductus pancreaticus'un anatomik varyasyonlarını belirlemektir. Eter anestezi uygulaması sonrası cavum abdominis açıldı. Kateter, vena iliecolica, duodenum ile jejenumun birleşim noktasına ve aorta'ya yerleştirildi. Tüm arter, ven ve pancreas kanalları sırasıyla kırmızı mavi ve beyaz boyalı lateks ile dolduruldu. Rat pankreası üç, tavşan pankreası ise iki lobdan oluşmaktaydı. Rat pankreası ligamentum gastrolienal ve mesoduodenum arasında yaygın bir bez olarak görüldü. Ductus pancreaticus anterior ve posterior olmak üzere iki kanal olduğu gözlendi. Bu kanalların başlangıcından itibaren sağ tarafta 8.09+2.65 mm sonra, sol tarafta da 7.32+3.61 mm sonra ductus biliopancreaticus'a açıldığı saptandı. Tavşan pankreası karaciğer, mide ve duodenum arasında yaygın bir bez olarak görülmekteydi. Tavşanda ductus pancreaticus, distal pylorus'ten 51.52+3.23 cm uzaklıkta duodenum'a açılır. Bunting & Jones (19) tarafından ductus pancreaticus'un tavşanda pylorus'un başlangıcından 25-27 cm sonra duodenum'a açıldığı bildirilmiştir. Bu çalışmada ise ductus pancreaticus'un açılış deliğinin pylorus'a uzaklığı 46.33-57.17cm olarak ölçüldü.

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Section: Resultssupporting

confidence: 62%

Section: Introductionmentioning

confidence: 99%

Morphological aspects of the pancreas in the rat and the rabbit: an investigation into the location, ducts, arteries and veins

Haligür

Karakurum

Dilek

2018

Veterinary Journal of Mehmet Akif Ersoy University

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“…Differences in fecal pH are expected to be smaller than differences in pH in the rumen due to lower fermentation intensity in the hindgut compared with the rumen (Dijkstra et al, 2012). In addition, the neutralization and buffering system in the hindgut (Sutton, 1985), primarily due to pancreatic secretions (Zabielski et al, 1997), contributes to regulation of hindgut digesta pH. Given that we did not measure fermentation in the rumen, we are unsure to what extent ruminal fermentation parameters changed in cows consuming forages with reduced particle size compared with the control group.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of fecal fermentation profile and bacterial community in organically fed dairy cows consuming forage-rich diets with different particle sizes

Castillo‐Lopez

Haselmann

Petri

et al. 2020

Journal of Dairy Science

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Organic cattle farming encourages the use of foragerich diets, and the reduction of particle size has been suggested as an approach to improve forage utilization and enhance nutrient intake of cows. However, reducing forage particle size increases passage rate, as well as the flow of potentially fermentable nutrients out of the rumen, and the consequences for hindgut fermentation have not been evaluated yet. This study evaluated the effects of decreasing dietary forage particle size on the fecal short-chain fatty acid (SCFA) profile and the bacterial community structure of dairy cows fed foragebased rations. Twenty-one organically fed lactating Holstein cows (4 primiparous and 17 multiparous; mean and standard deviation 703 ± 65 kg body weight, 135 ± 104 days in milk) were divided into 2 groups and fed 1 of 2 diets for 34 d. Diets contained 20% concentrate and 80% forage (dry matter basis), and were fed either as a control with a forage geometric mean particle size of 52 mm (CON; 11 cows) or as a diet with the forage particle size reduced to a geometric mean size of 7 mm (RED; 10 cows). Fecal samples were collected at the end of the experiment, and samples were immediately frozen at −20°C. Samples were analyzed for SCFA, and the fecal bacterial community was evaluated using 16S rRNA sequencing. Data showed that the concentration of total SCFA was not affected by treatment, but the proportion of propionate, a key glucogenic precursor in cattle, tended to be greater for RED (13.3 and 13.8 ± 0.1%, respectively). The predominant bacterial phyla, including Firmicutes (58.0 ± 0.7%), Bacteroidetes (26.9 ± 0.4%), and Verrucomicrobia (4.0 ± 0.4%), were not affected by forage particle size. Family Lachnospiraceae increased in relative abundance when the RED diet was fed (12.1 and 13.9 ± 0.5% for CON and RED, respectively), and genera Acetitomaculum (1.1 and 1.8 ± 0.2%), Turicibacter (0.7 and 0.9 ± 0.1%), and Ruminobacter (0.1 and 0.4 ± 0.1%) increased in relative abundance when RED was fed. In addition, relative abundance of some fecal bacterial taxa was correlated with major fecal SCFA and pH. Reducing the particle size of forages, from 52 to 7 mm geometric mean particle size, maintained fecal concentration of total SCFA and tended to enhance propionate concentration, without risk of dysbiosis. Thus, results suggest that reduction of forage particle size represents an effective approach to optimizing forage utilization while maintaining hindgut fermentation and fecal bacterial diversity in dairy cows fed forage-rich diets.

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“…Examples of functional resemblances are similar distribution of cholecystokinin (CCK) receptors and neuro-hormonal mechanisms controlling pancreatic juice secretion. The pancreatic juice drainage system is not much different, since in many pigs both main and accessory pancreatic ducts exist, though the accessory duct drains most of the porcine juice (77) . Pigs are omnivorous mammals and have a remarkable similarity to man in GIT anatomy, physiology, biochemistry (and even pathology) (78 -80) .…”

Section: Swine As a Model Of The Human Speciesmentioning

confidence: 99%

Nutritional programming of gastrointestinal tract development. Is the pig a good model for man?

Guilloteau

Zabielski²,

Hammon³

et al. 2010

Nutr. Res. Rev.

264

222

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The consequences of early-life nutritional programming in man and other mammalian species have been studied chiefly at the metabolic level. Very few studies, if any, have been performed in the gastrointestinal tract (GIT) as the target organ, but extensive GIT studies are needed since the GIT plays a key role in nutrient supply and has an impact on functions of the entire organism. The possible deleterious effects of nutritional programming at the metabolic level were discovered following epidemiological studies in human subjects, and confirmed in animal models. Investigating the impact of programming on GIT structure and function would need appropriate animal models due to ethical restrictions in the use of human subjects. The aim of the present review is to discuss the use of pigs as an animal model as a compromise between ethically acceptable animal studies and the requirement of data which can be interpolated to the human situation. In nutritional programming studies, rodents are the most frequently used model for man, but GIT development and digestive function in rodents are considerably different from those in man. In that aspect, the pig GIT is much closer to the human than that of rodents. The swine species is closely comparable with man in many nutritional and digestive aspects, and thus provides ample opportunity to be used in investigations on the consequences of nutritional programming for the GIT. In particular, the 'sow -piglets' dyad could be a useful tool to simulate the 'human mother-infant' dyad in studies which examine short-, middle-and long-term effects and is suggested as the reference model.

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Collection of pancreatic juice in experimental animals: mini-review of materials and methods

Cited by 9 publications

References 26 publications

Morphological aspects of the pancreas in the rat and the rabbit: an investigation into the location, ducts, arteries and veins

Morphological aspects of the pancreas in the rat and the rabbit: an investigation into the location, ducts, arteries and veins

Evaluation of fecal fermentation profile and bacterial community in organically fed dairy cows consuming forage-rich diets with different particle sizes

Nutritional programming of gastrointestinal tract development. Is the pig a good model for man?

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