Neopterin, a marker of immune response, and 8-hydroxy-2′-deoxyguanosine, a marker of oxidative stress, correlate at high age as determined by automated simultaneous high-performance liquid chromatography analysis of human urine

Svoboda, Peter; Ko, Seong Hee; Cho, Belong; Yoo, Sangjun; Choi, Su-Mi; Ye, Sang Kyu; Kasai, Hiroshi; Chung, Myung Hee

doi:10.1016/j.ab.2008.09.014

Cited by 33 publications

(17 citation statements)

References 25 publications

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“…28 The interferon-γ-activated monocytes/macrophages produce high levels of neopterin and also reactive oxygen species due to the immune activation. 29 Thus, neopterin may be used as a marker of both immune system activation and oxidative stress. 28,29 In this study, neopterin was correlated with Cu/Zn SOD but not with the inflammatory markers.…”

Section: Group B Diabetesmentioning

confidence: 99%

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Effect of diabetes and oxidative stress on plasma CCL23 levels in patients with severe chronic kidney disease

Pawlak¹,

Myśliwiec²,

Pawlak³

2014

Polish Archives of Internal Medicine

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Section: Group B Diabetesmentioning

confidence: 99%

“…29 Thus, neopterin may be used as a marker of both immune system activation and oxidative stress. 28,29 In this study, neopterin was correlated with Cu/Zn SOD but not with the inflammatory markers. However, the multiple regression analysis did not confirm neopterin as an independent factor associated with CCL23 levels in CKD patients.…”

Section: Group B Diabetesmentioning

confidence: 99%

Effect of diabetes and oxidative stress on plasma CCL23 levels in patients with severe chronic kidney disease

Pawlak¹,

Myśliwiec²,

Pawlak³

2014

Polish Archives of Internal Medicine

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“…NPT is a sensitive marker for the activity of inflammatory RA or OA [21]. In healthy adults, the average concentration of serum NPT was less than 2.23–2.46 μg/l, and its cutoff value was 3.04 μg/l [22]. Elevated NPT concentrations were useful for signalizing the activation of the human immune system.…”

Section: Discussionmentioning

confidence: 99%

The early diagnostic value of serum neopterin and cartilage oligomeric matrix protein for osteoarticular changes among brucellosis patients at an early period

Zhao

et al. 2018

J Orthop Surg Res

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BackgroundBrucellosis is an important public health problem in China. Brucellosis can cause many osteoarticular changes, especially chronic brucellosis. Brucellosis presents various diagnostic difficulties because it mimics many other diseases. Because of the poor sensitivity and ability of X-ray image, it is necessary to find a good method of early diagnosis for osteoarticular changes among brucellosis patients at an early period.The aim of this study was to find early changes biomarkers for osteoarticular changes among brucellosis patients at an early period and provide a better understanding of the osteoarticular changes in this disease at an early stage.MethodsSixty-one cases of brucellosis patients at an early period (within 6 months) and 67 cases of volunteers were selected and divided into brucellosis vs. control groups. Serum samples were detected with serological tests for brucellosis, including rose bengal plate test (RBPT), agglutination test (SAT), and IgG and IgM with ELISA. Meanwhile, cartilage oligomeric matrix protein (COMP) and neopterin (NPT) levels in all samples were measured simultaneously with ELISA. The data were analyzed using SPSS 17.0 software.ResultsTogether with the clinical examination, epidemiological investigation, and serological tests of RBPT, SAT, and IgG and IgM with ELISA, the patients from brucellosis group all were diagnosed as brucellosis cases at an early period, while the results of RBPT, SAT, and IgG and IgM with ELISA from the healthy control group were negative. Comparing with the healthy control, the medians of serum NPT and COMP in the patients group were 9.26 ng/l, 1.70 ng, respectively, which were higher than that of the healthy control group with significant differences (Z = 5.512, 3.614, all p = 0.000).ConclusionThe serum NPT and COMP levels of brucellosis patients at an early period were increased. The serum NPT and COMP levels might be the indicator biomarker for osteoarticular changes of human brucellosis at an early stage.

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“…For the measurement of NPT levels in serum or biological samples, analytical tools like high-performance liquid chromatography (HPLC) [4,5], capillary electrophoresis [6], fluoroimmunoassay (FIA) [7], radioimmunoassay (RIA) [8,9], and enzyme-linked immunosorbent assay (ELISA) [10,11] have been used. Although some of analytical techniques give sensitive and accurate result, they require the involvement of skilled personnel and expensive instrumentation.…”

Section: Introductionmentioning

confidence: 99%

A rapid detection of neopterin based on a label-free and homogeneous FRET immunoassay system

Kim

Shim

et al. 2013

Nano-Bio Sensing, Imaging, and Spectroscopy

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Herein, we have developed a label-free and homogeneous fluorescence resonance energy transfer (FRET) immunoassay for the detection of neopterin (NPT), which is an early and valuable biochemical marker of cellular immunity. Owing to intrinsic fluorescence properties of antibody and NPT, anti-NPT antibody (anti-NPT) and analyte played roles as the respective donor and acceptor in the FRET immunoassay. As the concentration of NPT increases, the fluorescence intensity at ~350 nm decreases owing to the formation of increasing amounts of the anti-NPT/NPT complex in which FRET takes place. The assay system was found to display a high specificity and a low detection limit (0.14 ng mL -1 ) for NPT. A practical application of the FRET immunoassay system was demonstrated by its use in the detection of NPT in spiked human serum samples. The observations made in these efforts show that the homogeneous FRET immunoassay strategy, which requires a simple sample preparation procedure, serves as a powerful tool for the rapid and sensitive quantitative determination of NPT.

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Neopterin, a marker of immune response, and 8-hydroxy-2′-deoxyguanosine, a marker of oxidative stress, correlate at high age as determined by automated simultaneous high-performance liquid chromatography analysis of human urine

Cited by 33 publications

References 25 publications

Effect of diabetes and oxidative stress on plasma CCL23 levels in patients with severe chronic kidney disease

Effect of diabetes and oxidative stress on plasma CCL23 levels in patients with severe chronic kidney disease

The early diagnostic value of serum neopterin and cartilage oligomeric matrix protein for osteoarticular changes among brucellosis patients at an early period

A rapid detection of neopterin based on a label-free and homogeneous FRET immunoassay system

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