Effect of diabetes and oxidative stress on plasma CCL23 levels in patients with severe chronic kidney disease

Pawlak, Krystyna; Myśliwiec, Michał; Pawlak, Dariusz

doi:10.20452/pamw.2405

Cited by 13 publications

(11 citation statements)

References 16 publications

(24 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…We measured the serum concentration of cytokines and chemokines in serum collected from CO and control subjects before, at day 1, and day 3 post-IAT. Subjects receiving CO islets had reduced myeloid progenitor inhibitory factor 1 (MPIF1 or CCL23) at day 1 post-transplantation (Figure 4(a)), a cytokine that often increased in subjects with inflammatory diseases 34,35 . Moreover, CO subjects had significantly higher CXCL12, a chemokine that can limit inflammation, at both day 1 and 3 post transplantation (Figure 4(b)).…”

Section: Resultsmentioning

confidence: 99%

“…CCL23 is a newly identified chemokine that can attract monocytes/macrophages, dendritic cells, lymphocytes, and endothelial cells, and can upregulate inflammatory cytokines such as TNF a and MCP-1 in human monocytes. Increased expression of CCL23 was found in patients with inflammatory bowel disease 34 , rheumatoid arthritis, and systemic sclerosis 35 . CXCL12 reduced inflammation in experimental autoimmune encephalomyelitis 38 .…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Islet Harvest in Carbon Monoxide-Saturated Medium for Chronic Pancreatitis Patients Undergoing Islet Autotransplantation

et al. 2019

View full text Add to dashboard Cite

Stresses encountered during human islet isolation lead to unavoidable b-cell death after transplantation. This reduces the chance of insulin independence in chronic pancreatitis patients undergoing total pancreatectomy and islet autotransplantation. We tested whether harvesting islets in carbon monoxide-saturated solutions is safe and can enhance islet survival and insulin independence after total pancreatectomy and islet autotransplantation. Chronic pancreatitis patients who consented to the study were randomized into carbon monoxide (islets harvested in a carbon monoxide-saturated medium) or control (islets harvested in a normal medium) groups. Islet yield, viability, oxygen consumption rate, b-cell death (measured by unmethylated insulin DNA), and serum cytokine levels were measured during the peri-transplantation period. Adverse events, metabolic phenotypes, and islet function were measured prior and at 6 months post-transplantation. No adverse events directly related to the infusion of carbon monoxide islets were observed. Carbon monoxide islets showed significantly higher viability before transplantation. Subjects receiving carbon monoxide islets had less b-cell death, decreased CCL23, and increased CXCL12 levels at 1 or 3 days post transplantation compared with controls. Three in 10 (30%) of the carbon monoxide subjects and none of the control subjects were insulin independent. This pilot trial showed for the first time that harvesting human islets in carbon monoxide-saturated solutions is safe for total pancreatectomy and islet autotransplantation patients.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Islet Harvest in Carbon Monoxide-Saturated Medium for Chronic Pancreatitis Patients Undergoing Islet Autotransplantation

et al. 2019

View full text Add to dashboard Cite

show abstract

“…18 It was also found that levels of CCL23 increased in patients with severe chronic kidney disease, which was correlated with kidney function. 19 However, CCL23 as a biomarker of cardiotoxicity has not been reported. It remains to be elucidated whether CCL23 contributes to the development of drug-induced cardiotoxicity.…”

Section: Discussionmentioning

confidence: 99%

Immune response proteins as predictive biomarkers of doxorubicin-induced cardiotoxicity in breast cancer patients

Cao

Makhoul

et al. 2017

Exp Biol Med (Maywood)

View full text Add to dashboard Cite

Cancer treatment with doxorubicin (DOX) can induce cumulative dose-dependent cardiotoxicity. Currently, there are no specific biomarkers that can identify patients at risk during the initial doses of chemotherapy. The aim of this study was to examine plasma cytokines/chemokines and potential cardiovascular biomarkers for the prediction of DOX-induced cardiotoxicity. Plasma samples were collected before (T0), and after the first (T1) and the second (T2) cycles of DOX-based chemotherapy of 27 breast cancer patients, including five patients who presented with >10% decline of left ventricular ejection fraction (LVEF), five patients with LVEF decline of 5-10%, and 17 patients who maintained normal LVEF at the end of chemotherapy (240 mg/m cumulative dose of DOX from four cycles of treatment). Multiplex immunoassays were used to screen plasma samples for 40 distinct chemokines, nine matrix metalloproteinases, 33 potential markers of cardiovascular diseases, and the fourth-generation cardiac troponin T assay. The results showed that the patients with abnormal decline of LVEF (>10%) had lower levels of CXCL6 and sICAM-1 and higher levels of CCL23 and CCL27 at T0; higher levels of CCL23 and lower levels of CXCL5, CCL26, CXCL6, GM-CSF, CXCL1, IFN-γ, IL-2, IL-8, CXCL11, CXCL9, CCL17, and CCL25 at T1; and higher levels of MIF and CCL23 at T2 than the patients who maintained normal LVEF. Patients with LVEF decline of 5-10% had lower plasma levels of CXCL1, CCL3, GDF-15, and haptoglobin at T0; lower levels of IL-16, FABP3, and myoglobin at T1; and lower levels of myoglobin and CCL23 at T2 as compared to the patients who maintained normal LVEF. This pilot study identified potential biomarkers that may help predict which patients are vulnerable to DOX-induced cardiotoxicity although further validation is needed in a larger cohort of patients. Impact statement Drug-induced cardiotoxicity is one of the major concerns in drug development and clinical practice. It is critical to detect potential cardiotoxicity early before onset of symptomatic cardiac dysfunction or heart failure. Currently there are no qualified clinical biomarkers for the prediction of cardiotoxicity caused by cancer treatment such as doxorubicin (DOX). By using multiplex immunoassays, we identified proteins with significantly changed plasma levels in a group of breast cancer patients who were treated with DOX-based chemotherapy and produced cardiotoxicity. These proteins were associated with immune response and were identified before DOX treatment or at early doses of treatment, thus they could be potential predictive biomarkers of cardiotoxicity although further validation is required to warrant their clinical values.

show abstract

“…The significantly expressed genes in blood transcriptome of SCA12 belonged to innate immunity. Among these, CCL23 is a member of the CC chemokine subfamily and GPR97 belongs to adhesion G-protein coupled receptor family reported as biomarker in inflammatory diseases (Pawlak et al, 2014). Similarly, LIMK2 is a member of PDZ/LIM family which has role in physiological functioning of nervous system pro-survival induced by DNA damage pathway and genotoxic stress (Cuberos et al, 2015).…”

Section: Signatures Of Inflammation In Both Sca12 Derived Neuronal LImentioning

confidence: 99%

Transcriptomic Dynamics of a non-coding trinucleotide repeat expansion disorder SCA12 in iPSC derived neuronal cells: signatures of interferon induced response

Kumar

Dhapola

et al. 2017

Preprint

View full text Add to dashboard Cite

Abstract:Spinocerebellar ataxia type-12 (SCA12) is a neurological disorder that exhibits a unique progressive tremor/ataxia syndrome induced by triplet (CAG) repeat expansion in 5' UTR of PPP2R2B. SCA12 is one of the most prominent SCA-subtype in India and till date no appropriate disease models have been described. Our aim was to establish human iPSC derived neuronal cell lines of SCA12 and study transcriptomic level alterations induced by CAG expansion. For translational application, peripheral blood transcriptomics of SCA12 patients was also performed. Lymphoblastoid cell lines of three SCA12 patients were reprogrammed to iPSCs and then re-differentiated into pan-neuronal lineage. RNAsequencing based comparative transcriptomics was performed for disease and control cell lineages. Microarray based transcriptomic profiling of peripheral blood of SCA12 patients was performed in a case/control (n=15/9) design. We have successfully created human neuronal cell lines of SCA12 patient as exhibited by their molecular profiling. Differential expression analysis of RNA-Seq data has shown enrichment for type-I interferon signaling and other relevant cellular processes in SCA12-neurons. At the splice-isoform level, we observed an upregulation of expanded CAG containing non-coding transcript of PPP2R2B.Peripheral blood transcriptomics analysis and targeted validation of RNA-Seq data has allowed us to identify inflammatory signatures as potential markers of molecular pathology in SCA12. Our study has allowed us to establish first iPSC based neuronal cell lines of SCA12.We have identified pro-inflammatory signatures in SCA12-neurons suggestive of a dsRNA mediated activation of interferon signaling and that corroborates with the emerging evidence of neuronal atrophy due to neuro-inflammation in common neurodegenerative diseases. This study involved development of an iPSCs derived neuronal cells of SCA12 and look through signatures of neurodegeneration by whole RNA sequencing. This model sheds light upon key role of RNA mediated induced response in Interferon signaling for neurodegeneration.peer-reviewed)

show abstract

Effect of diabetes and oxidative stress on plasma CCL23 levels in patients with severe chronic kidney disease

Cited by 13 publications

References 16 publications

Islet Harvest in Carbon Monoxide-Saturated Medium for Chronic Pancreatitis Patients Undergoing Islet Autotransplantation

Islet Harvest in Carbon Monoxide-Saturated Medium for Chronic Pancreatitis Patients Undergoing Islet Autotransplantation

Immune response proteins as predictive biomarkers of doxorubicin-induced cardiotoxicity in breast cancer patients

Transcriptomic Dynamics of a non-coding trinucleotide repeat expansion disorder SCA12 in iPSC derived neuronal cells: signatures of interferon induced response

Contact Info

Product

Resources

About