Nematodes

Bodri, Michael S.

doi:10.1002/9780470344606.ch15

Cited by 2 publications

(3 citation statements)

References 67 publications

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“…Thirty minutes in a refrigerator (+4ºC) is usually suffi cient for this purpose but other techniques can be effective; for example, nematodes can be chilled using a stream of carbon dioxide (Bodri 2006). However, hypothermia should not be used for surgical or other invasive procedures.…”

Section: Limited or Discontinued Methodsmentioning

confidence: 99%

“…Chitty (2006) described its use in myriapods and Pizzi (2006) in spiders, and Bodri (2006) included it in his extensive formulary of anesthetic agents for nematodes.…”

Section: Terrestrial Species: Inhalation Anesthesiamentioning

confidence: 99%

“…For some that have been widely used in research, such as the Nematoda (Bodri 2006), a whole range of techniques is available and there is extensive supporting literature. However, there is still, it appears, a dearth of information about the use of anesthetic agents in many groups.…”

Section: Anesthesiamentioning

confidence: 99%

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Anesthesia, Analgesia, and Euthanasia of Invertebrates

Cooper¹

2011

ILAR Journal

116

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Invertebrate animals have long played an important role in biomedical research in such fields as genetics, physiology, and development. However, with few exceptions, scientists, veterinarians, and technicians have paid little attention to the anesthesia, analgesia, and euthanasia of these diverse creatures. Indeed, some standard research procedures are routinely performed without anesthesia. Yet various chemical agents are available for the immobilization or anesthesia of invertebrates, ranging from gases or volatile liquids that can be pumped into either an anesthetic chamber (for terrestrial species) or a container of water (aquatic species), to benzocaine and other substances for fish. Many invertebrates are not difficult to immobilize or anesthetize and the procedures recommended in this article appear to be safe; however, none should be considered totally risk-free. Analgesia of invertebrates is as yet a largely unexplored field; until scientific data are available, other measures can promote the well-being of these animals in the laboratory. For euthanasia, various methods (physical or chemical or a combination of both) have been recommended for different taxa of invertebrates, but most have not been properly studied under laboratory conditions and some can be problematic in the context of research procedures and tissue harvesting. Furthermore, relevant data are scattered, sometimes available only in languages other than English, and there is no international approach for seeking and collating such information. In this article I review various methods of anesthesia, analgesia, and euthanasia for terrestrial and aquatic invertebrates, as well as areas requiring further research.

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Section: Limited or Discontinued Methodsmentioning

confidence: 99%

“…Chitty (2006) described its use in myriapods and Pizzi (2006) in spiders, and Bodri (2006) included it in his extensive formulary of anesthetic agents for nematodes.…”

Section: Terrestrial Species: Inhalation Anesthesiamentioning

confidence: 99%

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Anesthesia, Analgesia, and Euthanasia of Invertebrates

Cooper¹

2011

ILAR Journal

116

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Technologies for Micromanipulating, Imaging, and Phenotyping Small Invertebrates and Vertebrates

Yanik

Rohde²,

Pardo-Martín³

2011

Annu. Rev. Biomed. Eng.

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Small multicellular model organisms such as the invertebrate nematode C. elegans and the vertebrate zebrafish provide unique opportunities for both basic science and pharmaceutical discovery. In recent years, there have been significant breakthroughs in technologies to manipulate and image these organisms for a variety of purposes ranging from behavioral studies of neuronal circuits to high-throughput screening. Here, we review these advancements with a particular focus on the optically transparent model organisms C. elegans and zebrafish.

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Nematodes

Cited by 2 publications

References 67 publications

Anesthesia, Analgesia, and Euthanasia of Invertebrates

Anesthesia, Analgesia, and Euthanasia of Invertebrates

Technologies for Micromanipulating, Imaging, and Phenotyping Small Invertebrates and Vertebrates

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